Optimization of production conditions and 3D-structure modeling of novel antibacterial peptide of lantibiotic family
- Authors: Polyudova T.V.1, Lemkina L.M.1, Likhatskaya G.N.2, Korobov V.P.1,3,4
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Affiliations:
- Institute of Ecology and Genetics of Microorganisms
- G.B. Elyakov Pacific Institute of Bioorganic Chemistry
- Perm State National Research University
- Perm National Research Polytechnic University
- Issue: Vol 53, No 1 (2017)
- Pages: 40-46
- Section: Article
- URL: https://journals.rcsi.science/0003-6838/article/view/152144
- DOI: https://doi.org/10.1134/S0003683817010148
- ID: 152144
Cite item
Abstract
The effect of culture conditions (nutrient media, aeration, and temperature) of Staphylococcus warneri KL-1 on the growth and production of the antibacterial peptide warnerin was studied. The selected medium allowed the intensive development of S. warnerin KL-1 culture and a high level of peptide accumulation. It was demonstrated that the bacterial culture growth rate and warnerin production depended on the extent of aeration of the nutrient medium. The highest antibacterial activity (ABA) was achieved in culture liquid by the fed-batch cultivation. The amino acid sequence of the warnerin pure form was determined by structural bioinformatics analysis, and the 3D structure of this peptide was constructed. It was found that the peptide molecule contained three thioether cycles formed by one lanthionine residue and two methyl-lanthionine residues. According to the current classification of lanthipeptides, warnerin can be attributed to class I of lantibiotics.
About the authors
T. V. Polyudova
Institute of Ecology and Genetics of Microorganisms
Author for correspondence.
Email: poludova@iegm.ru
Russian Federation, Perm, 614081
L. M. Lemkina
Institute of Ecology and Genetics of Microorganisms
Email: poludova@iegm.ru
Russian Federation, Perm, 614081
G. N. Likhatskaya
G.B. Elyakov Pacific Institute of Bioorganic Chemistry
Email: poludova@iegm.ru
Russian Federation, Vladivostok, 690000
V. P. Korobov
Institute of Ecology and Genetics of Microorganisms; Perm State National Research University; Perm National Research Polytechnic University
Email: poludova@iegm.ru
Russian Federation, Perm, 614081; Perm, 614000; Perm, 614068
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