Analysis of the genotypes and interleukins 17A, 17F blood levels in children with allergic bronchial asthma and allergic rhinitis
- 作者: Prosekova E.1, Turyanskaya A.1, Dolgopolov M.1, Zhdanova O.2, Sabynych V.1
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隶属关系:
- Federal State Budgetary Educational Institution of Higher Education “Pacific State Medical University” of the Ministry of Healthcare of the Russian Federation
- FGBUN "Institute of Automation and Control Processes" of the Far Eastern Branch of the Russian Academy of Sciences
- 期: 卷 17, 编号 2 (2020)
- 页面: 61-68
- 栏目: Original studies
- URL: https://journals.rcsi.science/raj/article/view/121667
- DOI: https://doi.org/10.36691/RJA1360
- ID: 121667
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Introduction. The study of genes that control the activity of cytokines is one of the important issues in revealing the pathogenetic mechanisms of allergic diseases.
Aims. Determination of the frequency of occurrence of polymorphic gene markers genotypes and characterization of the interleukins 17A, 17F content in blood serum in children with bronchial asthma and allergic rhinitis.
Materials and methods. A comprehensive survey of 110 children with allergic diseases of 311 years old and 60 healthy peers. The material for genetic analysis was DNA with the study of mutation points of IL-17A at position 197 (G>A) and IL-17F 7488 (T>C). The content of IL-17A, IL-17F interleukins was measured by enzymelinked assay. For the statistical analysis we used the “Statistica 10”, methods for comparing unrelated groups of genotypes distributions to expected values at Hardy–Weinberg equilibrium with χ2.
Results. The frequency of occurrence of genotypes in a group of healthy children was as follows: IL-17A (G197A) – heterozygous GA (63,333%), homozygous GG (36,667%); IL-17F (T7488C) TT (36,667%), CT (63,333%), genotypes AA and CC weren’t determined. In children with allergic diseases, all genotypes were determined: IL-17A (G197A), GG (11,818%), AA (19,091%) and GA (69,091%), IL-17F (T7488C), TT (5,454%), CC (35,455%) and CT (59,091%) with the highest specific gravity of the GG genotype and TT. There were no significant differences in IL-17A, IL-17F levels in the blood serum depending on the genotype.
Discussion. There were significant differences in the structure of the polymorphisms of the IL-17A, IL-17F genes, blood levels of IL-17A, IL-17F and the risk of the disease in allergic children and healthy peers. The frequency of allergic diseases in children with genotypes AA and TT is statistically higher, but with genotypes GG, CC is statistically lower than with other genotypes.
作者简介
Elena Prosekova
Federal State Budgetary Educational Institution of Higher Education “Pacific State Medical University” of the Ministry of Healthcare of the Russian Federation
编辑信件的主要联系方式.
Email: pros.ev@mail.ru
ORCID iD: 0000-0001-6632-9800
Doctor of Medical Sciences, Full Professor, Head of Department of Clinical Laboratory Diagnostics and General and Clinical Immunology
俄罗斯联邦, 2, Prospekt OstryakovaVladivostok, 690002Alina Turyanskaya
Federal State Budgetary Educational Institution of Higher Education “Pacific State Medical University” of the Ministry of Healthcare of the Russian Federation
Email: alinakld@mail.ru
ORCID iD: 0000-0001-6993-9575
Teaching Assistant of Department of Clinical Laboratory Diagnostics and General and Clinical Immunology
俄罗斯联邦, 2, Prospekt OstryakovaVladivostok, 690002Maxim Dolgopolov
Federal State Budgetary Educational Institution of Higher Education “Pacific State Medical University” of the Ministry of Healthcare of the Russian Federation
Email: gades.med@mail.ru
ORCID iD: 0000-0003-4657-6868
Teaching Assistant of Department of Clinical Laboratory Diagnostics and General and Clinical Immunology
俄罗斯联邦, 690002, г. Владивосток, пр-кт Острякова, д. 2Oksana Zhdanova
FGBUN "Institute of Automation and Control Processes" of the Far Eastern Branch of the Russian Academy of Sciences
Email: axanka@iacp.dvo.ru
ORCID iD: 0000-0002-3090-986X
Doctor of Physical and Mathematical Sciences, senior research associate
俄罗斯联邦, 5, Radio str., Vladivostok, 690041Vitaly Sabynych
Federal State Budgetary Educational Institution of Higher Education “Pacific State Medical University” of the Ministry of Healthcare of the Russian Federation
Email: irinidi@mail.ru
ORCID iD: 0000-0003-3874-6433
Candidate of Medical Sciences, Associate Professor of Department of Clinical Laboratory Diagnostics and General and Clinical Immunology
俄罗斯联邦, 2, Prospekt OstryakovaVladivostok, 690002参考
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