Effect of lipoic acid on the exchange of sialo-containing compounds in rats blood plasma with alloxan diabet

封面

如何引用文章

全文:

详细

Oxidative stress is one of the markers of chronic diseases, including diabetes. Lipoic (tioctic) acid refers to natural non-enzyme antioxidants which is also necessary for the operation of multienzyme complexes catalyzing oxidative decarboxylation of pyruvate, α-ketoglutarate and other α-keto acids, and a poet omu plays an important role in the process of obtaining energy under aerobic conditions. Sialic acids are related to polyfunctional compounds that are part of various carbohydrate-containing compounds (glycoproteins, proteoglycans, gangliosides), including acute blood proteins.

The purpose of the study was a comparative study of the influence of lipoic acid lots on the exchange rates of compounds containing sialic acids in the blood plasma of rats with alloxan diabetes. Studies were conducted on white mongrel rats – males weighing 180-220 grams in the autumn-winter period. The animals were divided into three groups: the first – intact rats, in animals of the second and third groups, insulin-dependent diabetes mellitus (DM) was caused by a single subcutaneous injection of alloxan tetrahydrate. Rodents of the third group received lipoic acid intramuscularly. The degree of development of oxidative stress was assessed by the content of TBA-active products in the blood. Their indicators were compared with the concentrations of glucose, free, oligo- and protein-bound sialic acids in the blood on days 5, 10, 20, 30 and 40 of the experiments. It was found that on the background of the introduction of lipoic (thioctic) acid to experimental animals, there is a decrease in the level of glycemia, a decrease in the processes of oxidative stress and indicators of free and oligo-related sialic acids in blood plasma.

作者简介

Irina Volkhina

St. Petersburg State Pediatric Medical University

编辑信件的主要联系方式.
Email: volchinaiv@gmail.com

PhD, Associate Professor, Department of Biological Chemistry

俄罗斯联邦, Saint Petersburg

Evgenii Butolin

Izhevsk State Medical Academy

Email: butoline@mail.ru

MD, PhD, Dr Med Sci, Professor, Department of Biological Chemistry

俄罗斯联邦, Izhevsk

Elena Skvorsova

Izhevsk State Medical Academy

Email: skvor0280@mail.ru

graduate student of the Department of Biochemistry

俄罗斯联邦, Izhevsk

参考

  1. Волчегорский И.А., Рассохина Л.М., Мирошниченко И.Ю. Инсулинпотенцирующее действие антиоксидантов при экспериментальном сахарном диабете // Проблемы эндокринологии. - 2010. - Т. 56. - № 2. - С. 27-35. [Volchegorskiy IA, Rassokhina LM, Miroshnichenko IY. Insulin-potentiating action of antioxidants in experimental diabetes mellitus. Problems of endocrinology. 2010;56(2):27-35. (In Russ.)]. doi: 10.14341/probl201056227-35.
  2. Дедов И.И. Сахарный диабет - опаснейший вызов мировому сообществу // Вестник Российской академии медицинских наук. - 2012. - Т. 67. - № 1. - С. 7-13. [Dedov II. Diabetes mellitus - a dangerous treat to the mankind. Vestn Ross Akad Med Nauk. 2012;67(1):7-13. (In Russ.)]
  3. Занозина О.В., Рунов Г.П., Боровков Н.Н., Сорокина Ю.А. Альфа-липоевая кислота в коррекции электромиографических характеристик диабетической дистальной полинейропатии: фокус на маркеры окислительного стресса // Эффективная фармакотерапия. - 2015. - № 32. - C. 10-14. [Zanozina OV, Runov GP, Borovkov NN, Sorokina YA. Alpha-Lipoic Acid in Correction of Electromyographic Parameters of Diabetic Distal Polyneuropathy: the Markers of Oxidative Stress Emphasized. Effektivnaya farmakoterapiya. 2015;(32):10-14. (In Russ.)]
  4. Зайчик А.Ш., Чурилов Л.П. Патохимия (эндокринно-метаболические нарушения). - СПб., 2007. [Zaychik AS, Churilov LP. Patokhimiya (endokrinno-metabolicheskie narusheniya). Saint Petersburg; 2007. (In Russ.)]
  5. Климонтов В.В., Тян Н.В., Фазуллина О.Н., и др. Белки острой фазы воспаления и адипоцитокины в сыворотке крови у женщин с сахарным диабетом 2-го типа: взаимосвязи с составом тела и колебаниями уровня глюкозы в крови // Терапевтический архив. - 2016. - Т. 88. - № 10. - C. 35-41. [Klimontov VV, Tyan NV, Fazullina ON, et al. Acute-phase serum proteins and adipocytokines in women with type 2 diabetes mellitus: Relationships with body composition and blood glucose fluctuations. Terapeutic Arkhive. 2016;88(10):35-41. (In Russ.)]
  6. Лазуткин М.Н., Намоконов Е.В., Мироманов А.М., и др. Диагностическая значимость цитокинов и белков острой фазы в прогнозе гнойно-воспалительных осложнений стопы у больных сахарным диабетом // Сибирский медицинский журнал (Иркутск). - 2009. - Т. 91. - № 8. - C. 21-23. [Lazutkin MN, Namokonov EV, Miromanov AM, et al. Diagnostic significance of cytokines and proteins of acute phase in the prognosis of development of purulent-inflammatory complications of foot in the patients with diabetes mellitus. Sibirskii meditsinskii zhurnal (Irkutsk, Russia). 2009;91(8):21-23. (In Russ.)]
  7. Миллер Д.А., Миллер Т.М., Некрасова И.Л., и др. Сиаловые кислоты - показатель активности воспаления СОЖ у больных хроническим гастритом // Экспериментальная и клиническая гастроэнтерология. - 2013. - № 10. - C. 36-37. [Miller DA, Miller TM, Nekrasova IL, et al. Sialovye kisloty - pokazatel’ aktivnosti vospaleniya SOZh u bol’nykh khronicheskim gastritom. Experimental and clinical gastroenterology. 2013;(10):36-37. (In Russ.)]
  8. Пальчикова Н.А., Селятицкая В.Г., Шорин Ю.П. Количественная оценка чувствительности экспериментальных животных к диабетогенному действию аллоксана // Проблемы эндокринологии. - 1987. - Т. 33. - № 4. - C. 65-68. [Pal’chikova NA, Selyatitskaya VG, Shorin YP. Quantitative assessment of the sensitivity of experimental animals to the diabetogenic action of alloxan. Problems of endocrinology. 1987;33(4):65-68. (In Russ.)]
  9. Протасова С.В., Бутолин Е.Г., Оксузян А.В. Обмен углеводсодержащих биополимеров в печени и слизистой желудка при экспериментальном диабете у крыс с различной устойчивостью к стрессу // Сахарный диабет. - 2010. - Т. 13. - № 1. - C. 10-12. [Protasova SV, Butolin EG, Oksuzyan AV. Metabolism of carbohydrate-containing biopolymers in liver and gastric mucosa of rats with experimental diabetes and varying stress resistance. Diabetes mellitus. 2010;13(1):10-12. (In Russ.)]. doi: 10.14341/2072-0351-6010.
  10. Строков И.А., Фокина А.С. Альфа-липоевая кислота - основное фармакологическое лечение диабетической полиневропатии в стационаре и поликлинике // Медицинский совет. - 2016. - № 17. - C. 28-33. [Strokov IA, Fokina AS. α-Lipoic acid as the main pharmacological drug for in- and outpatient treatment of diabetic polyneuropathy. Meditsinskiy sovet. 2016;(17):28-33. (In Russ.)]
  11. Kocak G, Aktan F, Canbolat O, et al. Alpha-lipoic acid treatment ameliorates metabolic parameters, blood pressure, vascular reactivity and morphology of vessels already damaged by streptozotocin-diabetes. Diabetes Nutr Metab. 2000;13(6):308-318.
  12. Rajappa M, Ikkruthi S, Nandeesha H, et al. Relationship of raised serum total and protein bound sialic acid levels with hyperinsulinemia and indices of insulin sensitivity and insulin resistance in non-diabetic normotensive obese subjects. Diabetes Metab Syndr. 2013;7(1):17-19. doi: 10.1016/j.dsx.2013.02.030.
  13. Ziegler D. Thioctic acid for patients with symptomatic diabetic polyneuropathy: a critical review. Treat Endocrinol. 2004;3(3):173-189. doi: 10.2165/00024677-200403030-00005.

版权所有 © Volkhina I.V., Butolin E.G., Skvorsova E.A., 2018

Creative Commons License
此作品已接受知识共享署名 4.0国际许可协议的许可
 


##common.cookie##