Changes in the Deformability and Resistance of Erythrocyte Membranes under the Action of Gamma-Aminobutyric Acid Derivatives in the Offspring of Rats with Experimental Preeclampsia
- Authors: Muzyko E.A.1, Naumenko L.V.1, Perfilova V.N.1,2, Zavadskaya V.E.1, Varlamova S.V.1, Tyurenkov I.N.1, Vasil'yeva O.S.3
-
Affiliations:
- Volgograd State Medical University
- Scientific Center for Innovative Medicines
- The Herzen State Pedagogical University of Russia
- Issue: Vol 30, No 1 (2022)
- Pages: 13-20
- Section: Original study
- URL: https://journals.rcsi.science/pavlovj/article/view/75789
- DOI: https://doi.org/10.17816/PAVLOVJ75789
- ID: 75789
Cite item
Abstract
INTRODUCTION: Preeclampsia is a severe complication of pregnancy associated with the negative consequences for the mother and child. Such complications can be a reduction in the resistance of erythrocyte membranes to damaging agents and alteration of rheological properties of the blood in offspring. Promising compounds for the correction of these negative consequences of preeclampsia are gamma-aminobutyric acid (GABA) derivatives, which showed membrane–protective, antioxidant, and antihypoxic effects in previous studies.
AIM: To evaluate the effect of GABA derivatives Succicard® (4-phenylpiracetam and ethane-1,2-dicarboxylic acid, 2:1), Salifen® (4-amino-3-phenylbutanoic acid and 2-hydroxybenzoic acid, 2:1), and Phenibut® (aminophenylbutyric acid) on the deformability and resistance of erythrocyte membranes in 8- and 14-month-old offspring of rats with experimental preeclampsia (EP).
MATERIALS AND METHODS: The study involved offspring (male and female) of white non-inbred female rats with a normal pregnancy and EP that was modeled by replacement of drinking water with a 1.8% sodium chloride solution during gestation (1–21 days). For 30 days (from day 40 to day 70 of life), pup rats intragastrically received Succicard® (22 mg/kg), Salifen® (7.5 mg/kg), and Phenibut® (25 mg/kg), along with a comparison drug Pantogam® (calcium gopantenate) (50 mg/day) once a day. The offspring of the positive and negative control groups were injected with distilled water in a similar mode. In offspring aged 8 and 14 months, the resistance of erythrocyte membranes to the action of hydrochloric acid and their deformability were determined.
RESULTS: In 8-month-old male offspring of rats with EP, a shorter time of achievement of half the maximal amplitude of erythrogram was noted in acid hemolysis relative to the positive control group, and the erythrocyte elongation index was reduced. Relative to the negative control group, Succicard®, Salifen®, Phenibut®, and Pantogam® promoted the prolongation of hemolysis and the erythrocyte elongation index in 8-month-old male rats in the experimental groups. In 14-month-old male and female rats of different ages, no statistically significant differences were found between the groups.
CONCLUSION: Changes in the stiffness and strength of erythrocyte membranes were noted only in male offspring of rats with EP. Succicard®, Salifen®, Phenibut®, and Pantogam® produced membrane-protective effects on the erythrocytes of 8-month-old male rats of the experimental groups.
Full Text
##article.viewOnOriginalSite##About the authors
Elena A. Muzyko
Volgograd State Medical University
Author for correspondence.
Email: muzyko.elena@mail.ru
ORCID iD: 0000-0003-0535-9787
SPIN-code: 9939-9414
MD, Cand. Sci. (Med.), assistant at the department of theoretical biochemistry with a course in clinical biochemistry
Russian Federation, VolgogradLyudmila V. Naumenko
Volgograd State Medical University
Email: milanaumenko@mail.ru
ORCID iD: 0000-0002-2119-4233
SPIN-code: 8537-0991
MD, Associate Professor, doctor of science (Medicine), assistant professor, professor of the department of pharmacology and bioinformatics
Russian Federation, VolgogradValentina N. Perfilova
Volgograd State Medical University; Scientific Center for Innovative Medicines
Email: vnperfilova@mail.ru
ORCID iD: 0000-0002-2457-8486
SPIN-code: 3291-9904
doctor of science (Biology), professor, professor of the department of pharmacology and pharmacy
Russian Federation, Volgograd; VolgogradValeriya E. Zavadskaya
Volgograd State Medical University
Email: valzavadskaya@yandex.ru
ORCID iD: 0000-0003-0772-8371
student of 4 course of the Pharmacy Faculty
Russian Federation, VolgogradSof’ya V. Varlamova
Volgograd State Medical University
Email: varlamovasophia@yandex.ru
ORCID iD: 0000-0002-4242-9863
student of 4 course of the Pharmacy Faculty
Russian Federation, VolgogradIvan N. Tyurenkov
Volgograd State Medical University
Email: fibfuv@mail.ru
ORCID iD: 0000-0001-7574-3923
SPIN-code: 6195-6378
HFRHE RF, HSW RF, corresponding member of RAS, MD, doctor of science (Medicine), professor, head of the department of pharmacology and pharmacy of Institute of Continuing Medical and Pharmaceutical Education
Russian Federation, VolgogradOl’ga S. Vasil'yeva
The Herzen State Pedagogical University of Russia
Email: kohrgpu@yandex.ru
ORCID iD: 0000-0002-7779-8861
SPIN-code: 9820-9881
candidate of science (Chemistry), senior researcher of Problem Laboratory of Nitro Compounds, Department of Organic Chemistry
Russian Federation, Saint-PetersburgReferences
- Lu HQ, Hu R. Lasting Effects of Intrauterine Exposure to Preeclampsia on Offspring and the Underlying Mechanism. American Journal of Perinatology Reports. 2019;9(3):e275-91. doi: 10.1055/s-0039-1695004
- Turbeville HR, Sasser JM. Preeclampsia beyond pregnancy: long-term consequences for mother and child. American Journal of Physiology. Renal Physiology. 2020;318(6):F1315–26. doi: 10.1152/ajprenal.00071.2020
- Silvestro S, Calcaterra V, Pelizzo G, et al. Prenatal Hypoxia and Placental Oxidative Stress: Insights from Animal Models to Clinical Evidences. Antioxidants. 2020;9(5):414. doi: 10.3390/antiox9050414
- Oborin VA, Ashikhmina TYa. Experimental substantiation of the possibility of using red blood cells as a model for studying the membrane damaging effect of nanoparticles. Theoretical and Applied Ecology. 2020;(3):176–81. (In Russ). doi: 10.25750/1995-4301-2020-3-176-181
- Yakovlev DS, Naumenko LV, Sultanova KT, et al. Hemorheological properties of the 5-HT2A-Antagonist of the 2-methoxyphenyl-imidazobenzimidazole derivative of the RU-31 compound and cyproheptadine, in comparison with penthoxyphylline. Pharmacy & Pharmacology. 2020;8(5):345–53. (In Russ). doi: 10.19163/2307-9266-2020-8-5-345-353
- Tyurenkov IN, Popova TA, Perfilova VN, et al. Effect of RSPU-189 Compound and Sulodexide on Placental Mitochondrial Respiration in Female Rats with Experimental Preeclampsia. SOJ Gynecology, Obstetrics and Women's Health. 2016;2(2):7. doi: 10.15226/2381-2915/2/2/00112
- Nazarov SB, Ivanova AS, Novikov AA. The role of nitric oxide in regulation of the erythrocyte system state in rat offspring with chronic disturbance of uteroplacental blood circulation. Experimental and Clinical Pharmacology. 2012;75(5):21–3. (In Russ). doi: 10.30906/0869-2092-2012-75-5-21-23
- Revin VV, Gromova NV, Revina ES, et al. Effects of Polyphenol Compounds and Nitrogen Oxide Donors on Lipid Oxidation, Membrane–Skeletal Proteins, and Erythrocyte Structure Under Hypoxia. BioMed Research International. 2019;2019: 6758017. doi: 10.1155/2019/6758017
- Diederich L, Suvorava T, Sansone R, et al. On the Effects of Reactive Oxygen Species and Nitric Oxide on Red Blood Cell Deformability. Frontiers in Physiology. 2018;9:332. doi: 10.3389/fphys.2018.00332
- Muzyko EA, Kustova MV, Suvorin KV, et al. Change of the oxidant and antioxidant status in the offspring from rats with experimental preeclampsia under the effect of GABA derivatives. Journal of Volgograd State Medical University. 2020;(1):98–101. (In Russ). doi: 10.19163/1994-9480-2020-1(73)-98-101
- Tyurenkov IN, Perfilova VN. Kardiovaskulyarnyye i kardioprotektornyye svoystva GAMK i eye analogov. Volgograd: Izdatel’stvo VolGMU; 2008. (In Russ).
- Doucet DR, Bonitz RP, Feinman R, et al. Estrogenic hormone modulation abrogates changes in red blood cell deformability and neutrophil activation in trauma hemorrhagic shock. The Journal of Trauma. 2010;68(1):35–41. doi: 10.1097/TA.0b013e3181bbbddb
- Farber PL, Freitas T, Saldanha C, et al. Beta-estradiol and ethinylestradiol enhance RBC deformability dependent on their blood concentration. Clinical Hemorheology and Microcirculation. 2018;70(3):339–45. doi: 10.3233/CH-180392
- Mladenović J, Ognjanović B, Dordević N, et al. Protective effects of oestradiol against cadmium-induced changes in blood parameters and oxidative damage in rats. Archives of Industrial Hygiene and Toxicology. 2014;65(1):37–46. doi: 10.2478/10004-1254-65-2014-2405