Species diversity of vaginal lactobacilli in norm and in dysbiotic states

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Introduction. In healthy women of reproductive age, the vaginal microflora is represented mainly by lactobacilli. They provide a barrier function, preventing the propagation of opportunistic pathogens and the colonization of the vagina by pathogenic microorganisms. It is shown that the lactobacillary microflora of the vagina is very diverse, but not all species of lactobacilli can provide reliable protection of the female organism.

Objective: to characterize the species and quantitative composition of vaginal lactobacilli of women of reproductive age in norm and in dysbiosis.

Material and methods. The study involved 123 patients from polyclinic departments of the D.O. Ott Reasearch Institute of Obstetrics, Gynecology and Reproductology. For analysis of clinical material (vaginal discharge) for lactobacilli and other microorganisms, quantitative real-time PCR was used.

Results. The most common types of lactobacilli are Lactobacillus jensenii, L. Iners, L. crispatus, L. vaginalis and L. gasseri. Species diversity of lactobacilli (detection of ≥2 species) is observed much more often in women with physiological microbiocenosis than in women with vaginal dysbiosis. Our study confirms that L. crispatus is the dominant species of the vaginal biotope of healthy women, while in dysbiosis the species L. gasseri and L. Iners are most often identified.

作者简介

Olga Budilovskaya

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

编辑信件的主要联系方式.
Email: o.budilovskaya@gmail.com

Researcher, Laboratory of Microbiology

俄罗斯联邦, Saint Petersburg, Russia

Elena Shipitsyna

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: shipitsyna@inbox.ru

Senior Researcher, Laboratory of Microbiology

俄罗斯联邦, Saint Petersburg, Russia

Ekaterina Gerasimova

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: iagmail@ott.ru

Researcher, Laboratory of Microbiology

俄罗斯联邦, Saint Petersburg, Russia

Margarita Safronova

North-western State Medical University named after I.I.Mechnikov

Email: kafedra.pro@mail.ru

MD, Professor, Head of the Department of Women’s Reproductive Health

俄罗斯联邦, Saint Petersburg, Russia

Alevtina Savicheva

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: savitcheva@mail.ru

Head of Laboratory of Microbiology

俄罗斯联邦, Saint Petersburg, Russia

参考

  1. Pavlova SI, Kilic AO, So JS, et aL. Genetic diversity of vaginal lactobacilli from women in different countries on 16S rRNA gene sequences. J App Microbiol. 2002;92(3):451-9.
  2. Кира Е.Ф. Бактериальный вагиноз. – М.: МИА, 2012. – 472 с. [Kira EF. Bacterial’nyi vaginoz. Moscow: Medicinskoe informacionnoe agentstvo; 2012. (In Russ.)]
  3. Wilks M, Wiggins R, Whiley A, et aL. Identification and H2O2 production of vaginal lactobacilli from pregnant women at high risk of preterm birth and relation with outcome. J Clin MicrobioL. 2004;42:713-7.
  4. Brotman RM, Bradford LL, Conrad M, et aL. Association between Trichomonas vaginalis and vaginal bacterial community composition among reproductive-age women. Sex Transm Dis. 2012;39:807-2. doi: 10.1097/OLQ.0b013e3182631c79.
  5. Mane A, Kulkarni S, Ghate M, et aL. HIV-1 RNA shedding in the female genital tract is associated with reduced quantity of Lactobacilli in clinically asymptomatic HIV-positive women. Diagn Microbiol Infect Dis. 2013;75:112-4. doi: 10.1016/j.diagmicrobio.2012.09.009.
  6. Giakoumelou S, Wheelhouse N, Cuschieri K, et aL. The role of infection in miscarriage. Hum Reprod Update. 2016;22(1):116-33. doi: 10.1093/humupd/dmv041.
  7. Zakaria Gomaa E. Antimicrobial and anti-adhesive properties of biosurfactant produced by lactobacilli isolates, biofilm formation and aggregation ability. J Gen Appl MicrobioL. 2013;59:425-36.
  8. Falsen E, Pascual C, Sjoden B, et aL. Phenotypic and phylogenetic characterization of a novel Lactobacillus species from human sources: description of Lactobacillus iners sp. nov. Int J Syst BacterioL. 1999;49(Pt 1):217-21.
  9. Antonio MAD, Hawes SE, Hillier SL. The identification of vaginal Lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species. J Infect Dis. 1999;180:1950-6.
  10. Rampersaud R, Planet PJ, Randis TM, et aL. Inerolysin, a cholesterol-dependent cytolysin produced by Lactobacillus iners. J BacterioL. 2011;193:1034-41.
  11. Macklaim JM, Fernandes AD, Di Bella JM, et aL. Comparative meta-RNA-seq of the vaginal microbiota and differential expression by Lactobacillus iners in health and dysbiosis. Microbiome. 2013;1:12. doi: 10.1186/2049-2618-1-12.
  12. Donders GG, Van CK, Bellen G, et aL. Predictive value for preterm birth of abnormal vaginal flora, bacterial vaginosis and aerobic vaginitis during the first trimester of pregnancy. BJOG. 2009;116:1315-1324. doi: 10.1111/j.1471-0528.2009.2237.x.
  13. Ravel J, Gajer P, Abdo Z, et aL. Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci USA. 2011;108Suppl 1:4680-7. doi: 10.1073/pnas.1002611107.
  14. Исаева А.С., Летаров А.В., Ильина Е.Н., и др. Видовая идентификация влагалищных лактобацилл, выделенных у женщин репродуктивного возраста // Акушерство и гинекология. – 2012. – № 3. – С. 60–64. [Isaeva AS, Letarov AV, Il’ina EN, et aL. Vidovaja identifikacija vlagalishhnyh laktobacill, vydelennyh u zhenshhin reproduktivnogo vozrasta. Akusherstvo i ginekologija. 2012;(3):60-64. (In Russ.)]
  15. Мелкумян А.Р., Припутневич Т.В. Влагалищные лактобактерии — современные подходы к видовой идентификации и изучению их роли в микробном сообществе // Акушерство и гинекология. – 2013. – № 7. – С. 18–23. [Melkumyan AR, Priputnevich TV. Vaginal lactobacilli: current approaches to species identification and to the study of their role in the microbial community. Obstetrics and Gynecology. 2013;(7):18-23. (In Russ.)]
  16. Petrova MI, van den Broek M, Balzarini J, et aL. Vaginal microbiota and its role in HIV transmission and infection. FEMS Microbiol Rev. 2013;37(5):762-92. doi: 10.1111/1574-6976.12029.
  17. Yamamoto T, et aL. Bacterial population in the vaginas of healthy adolescent women. J Pediatr Adolesc GynecoL. 2009;22:11-8.
  18. Lamont RF, Sobel JD, Akins RA, et aL. The vaginal microbiome: new information about genital tract flora using molecular based techniques. BJOG. 2011Apr;118(5):533-49. doi: 10.1111/j.1471-0528.2010.02840.x.
  19. Jespers V, van de Wijgert J, Cools P, et aL. The significance of Lactobacillus crispatus and L. vaginalis for vaginal health and the negative effect of recent sex: a cross-sectional descriptive study across groups of African women. BMC Infect Dis. 2015;15:115. doi: 10.1186/s12879-015-0825-z.
  20. Breshears LM, Edwards VL, Ravel J, Peterson ML. Lactobacillus crispatus inhibits growth of Gardnerella vaginalis and Neisseria gonorrhoeae on a porcine vaginal mucosa modeL. BMC MicrobioL. 2015;15:276.
  21. DOE Joint Genome Institute http://genome.jgi-psf.org/finished_microbes/lacga/lacga.home.html Accessed 8/23/07 21.

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版权所有 © Budilovskaya O.V., Shipitsyna E.V., Gerasimova E.N., Safronova M.M., Savicheva A.M., 2017

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