Assessment of current methods of laboratory diagnosis of bacterial vaginosis

封面


如何引用文章

全文:

详细

41 women with polycystic ovary syndrome (PCOS) and 15 healthy women of reproductive age were examined to evaluate ovarian aromatase activity. Aromatase activity was determined by the decrease of estradiol level after peroral intake of aromatase inhibitor letrosol. To examine aromatase activity of antral follicle (∆E2) was divided on the blood level of antimullerian hormone (AMH), which is corresponded to the number of antral follicles. Significant variations of aromatase activity of antral follicles in patients with PCOS were determined: in 34.1 % of women it was within physiological ranges, in 48.8 % of women it was decreased and in 17.1 % of women it was increased. Aromatase activity of antral follicles in patients with PCOS correlated with blood levels of estradiol (r = 0.67), estron (r = 0.27), free testosterone(r = 0.43), androstendion (r = 0.34) and body mass index (r = 0.30). Aromatase activity had reverse correlation with number of antral follicles. Athors suggest that the sensitivity of the ovaries to gonadotropinic stimulation is decreased in patients with PCOS and low aromatase activity.

作者简介

Kira Shalepo

D. O. Ott Research Institute of Obstetrics and Gynecology, RAMS

Senior Researcher, Laboratory of Microbiology, PhD

Veronika Nazarova

D. O. Ott Research Institute of Obstetrics and Gynecology, RAMS

Bacteriologist, Laboratory of Microbiology

Yulia Menukhova

City Clinic 32, Women’s Consultation N 14

Head of Women’s Consultation N 14

Tatiana Rumyantseva

Central Research Institute for Epidemiology of Russia

Researcher, Laboratory for Molecular Diagnostics and Epidemiology of Reproductive Tract Infections

Alexander Guschin

Central Research Institute for Epidemiology of Russia

Head of the Laboratory for Molecular Diagnostics and Epidemiology of Reproductive Tract Infections, PhD

Alevtina Savicheva

D. O. Ott Research Institute of Obstetrics and Gynecology, RAMS

Email: savitcheva@mail.ru
MD, Professor, Head of the Laboratory of Microbiology

参考

  1. Домейка М., Савичева А. М., Соколовский Е., Баллард Р., Унемо М. Руководство по лабораторной диагностике инфекций урогенитального тракта. СПб.: Н-Л; 2012.
  2. Amsel R., Totten P. A., Spiegel C. A., Chen K. C., Eschenbach D., Holmes K. K. Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations. Am. J. Med. 1983; 74 (1): 14-22.
  3. Anderson M. R., Karasz A. How do clinicians manage vaginal complaints? An internet survey. Med. Gen. Med. 2005; 7 (2): 61.
  4. Cartwright C. P., Lembke B. D., Ramachandran K., Body B. A., Nye M. B., Rivers C. A., Schwebke J. R. Development and validation of a semiquantitative, multitarget PCR assay for diagnosis of bacterial vaginosis. J. Clin. Microbiol. 2012; 50 (7): 2321-9.
  5. Danielsson D., Teigen P. K., Moi H. The genital econiche: focus on microbiota and bacterial vaginosis. Annals of the New York Academy of Sciences. Ann. N. Y. Acad. Sci. 2011; 1230: 48-58.
  6. Fredricks D. N., Fiedler T. L., Thomas K. K., Oakley B. B., Marrazzo J. M. Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis. J. Clin. Microbiol. 2007; 45 (10): 3270-6.
  7. Haggerty C. L., Hillier S. L., Bass D. S., Ness R. B. Bacterial vaginosis and anaerobic bacteria are associated with endometritis. Clin. Infect. Dis. 2004; 39 (7): 990-5.
  8. Hallen A., Påhlson C., Forsum U. Bacterial vaginosis in women attending STD clinic: diagnostic criteria and prevalence of Mobiluncus spp. Genitourin. Med. 1987; 63 (6): 386-9.
  9. Hillier S. L., Nugent R. P., Eschenbach D. A., Krohn M. A., GibbS R. S., Martin D. H., Cotch M. F., Edelman R., Pastorek J. G. 2nd, Rao A. Association between, bacterial vaginosis and preterm delivery of a low-birth-weight infant. The Vaginal Infections and Prematurity Study Group. N. Engl. J. Med. 1995; 333 (26): 1737-42.
  10. Ison C. A., Hay P. E. Validation of a simplified grading of Gram stained vaginal smears for use in genitourinary medicine clinics. Sex. Transm. Infect. 2002; 78 (6): 413-5.
  11. Klebanoff S. J., Hillier S. L., Eschenbach D. A., Walterdorph A. M. Control of the microbial flora of the vagina by H202-generating lactobacilli J. Infect. Dis. 1991; 164 (1): 94-100.
  12. Koumans E. H., Sternberg M., Bruce C., McQuillan G., Kendrick J., Sutton M., Markowitz L. E. The prevalence of bacterial vaginosis in the United States, 2001-2004; associations with symptoms, sexual behaviors, and reproductive health. Sex. Transm. Dis. 2007; 34 (11): 864-9.
  13. Leitich H., Kiss H. Asymptomatic bacterial vaginosis and intermediate flora as risk factors for adverse pregnancy outcome. Best. Pract. Res. Clin. Obstet. Gynaecol. 2007; 21 (3): 375-390.
  14. Ling Z., Kong J., Liu F., Zhu H., Chen X., Wang Y., Li L., Nelson K. E., Xia Y., Xiang C. Molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis. BMC Genomics. 2010; 11: 488.
  15. Menard J. P., Fenollar F., Henry M., Bretelle F., Raoult D. Molecular quantification of Gardnerella vaginalis and Atopobium vaginae loads to predict bacterial vaginosis. Clin. Infect. Dis. 2008; 47: 33-43.
  16. Mittal V., Jain A., Pradeep Y. Development of modified diagnostic criteria for bacterial vaginosis at peripheral health centres in developing countries. J. Infect. Dev. Ctries. 2012; (5): 373-7.
  17. Nugent R. P., Krohn M. A., Hillier S. L. Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. J. Clin. Microbiol. 1991; 29 (2): 297-301.
  18. Oakeshott P., Hay P., Hay S., Steinke F., Rink E., Kerry S. Association between bacterial vaginosis or chlamydial infection and miscarriage before 16 weeks' gestation: prospective community based cohort study. BMJ 2002; 325 (7376): 1334.
  19. Sha B. E., Chen H. Y., Wang Q. J., Zariffard M. R., Cohen M. H., Spear G. T. Utility of Amsel criteria, Nugent score, and quantitative PCR for Gardnerella vaginalis, Mycoplasma hominis and Lactobacillus spp. for diagnosis of bacterial vaginosis in human immunodeficiency virus-infected women. J. Clin. Microbiol. 2005; 43 (9): 4607-4612.
  20. Shipitsyna E., Roos A., Datsu R., Hallen A., Fredlund H., Jensen J. S., Engstrand L., Unemo M. Composition of the vaginal microbiota in women of reproductive age - sensitive and specific molecular diagnosis of bacterial vaginosis is possible? PLoS One. 2013;8 (4): e60670.
  21. Spiegel C. A., Amsel R., Holmes К. K. Diagnosis of bacterial vaginosis by direct gram stain of vaginal fluid. J. Clin. Microbiol. 1983; 18 (1): 170-7.
  22. Swidsinski A., Mendling W., Loening-Bauske V., Ladhoff A., Swidsinski S., Hale L. P., Lochs H. Adherent biofilms in bacterial vaginosis. Obstet. Gynecol. 2005;106 (5):1013-23.

版权所有 © Shalepo K.V., Nazarova V.V., Menukhova Y.N., Rumyantseva T.A., Guschin A.E., Savicheva A.M., 2014

Creative Commons License
此作品已接受知识共享署名 4.0国际许可协议的许可

##common.cookie##