Analysis of sperm DNA fragmentationin infertility patients

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Using Terminal Deoxynucleotidyl Transferase dUTP Nick End Labelling (TUNEL) technique the assessment of the frequency of the spermatozoa with fragmented DNA in the ejaculate from sperm donors and men with infertility was analyzed. The DNA fragmentation rate was higher in spermatozoa of carriers of a chromosomal structural abnormality compared with the control group. There was no correlation between the sperm DNA fragmentation rate and the parameters of semen analysis. The direct linear correlation between the frequency of the spermatozoa with fragmented DNA and vacuole sperm head was found. The DNA fragmentation rate was not correlated to the frequency of the spermatozoa with bulb, amorphous heads or spermatozoa with abnormal acrosome

作者简介

Evgeniya Shilnikova

D. O. Ott Research Institute of Obstetrics and Gynecology NWD RAMS

Email: iagmail@ott.ru
PhD student, department of genetics and breeding, Saint-Petersburg State University; department of artificial redproductive technologies, D. O. Ott Research Institute of Obstetrics and Gynecology NWD RAMS

Irina Fedorova

D. O. Ott Research Institute of Obstetrics and Gynecology NWD RAMS

Email: irendf@mail.ru
PhD, senior Researcher, embryologist, department of artificial redproductive technologies

Alexander Gzgzyan

D. O. Ott Research Institute of Obstetrics and Gynecology NWD RAMS

Email: iagmail@ott.ru
Full Doctor, the head of the department of of artificial redproductive technologies

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