Gardnerella vaginalis: genotypic and phenotypic diversity, virulence factors and role in the pathogenesis of bacterial vaginosis

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This review summarizes literature data on the structure, biological characteristics, and virulence factors of Gardnerella vaginalis. Genotypic and phenotypic diversity of the bacterium, as well as its role in bacterial vaginosis are highlighted.

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Anna Krysanova

The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott; Saint Petersburg State Pediatric Medical

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Email: krusanova.anna@mail.ru

Researcher. The Laboratory of Microbiology, The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott, Saint Petersburg, Russia; Assistant. The Department of Clinical Laboratory Diagnostics, Faculty of Postgraduate and Additional Professional Education, Saint Petersburg State Pediatric Medical University

俄罗斯联邦, Saint Petersburg

参考

  1. Catlin BW. Gardnerella vaginalis: characteristics, clinical considerations, and controversies. Clin Microbiol Rev. 1992;5(3):213-237. https://doi.org/10.1128/cmr.5.3.213.
  2. Piot P, van Dyck E, Goodfellow M, Falkow S. A taxonomic study of Gardnerella vaginalis (Haemophilus vaginalis) Gardner and Dukes 1955. J Gen Microbiol. 1980;119(2):373-396. https://doi.org/10.1099/00221287-119-2-373.
  3. Greenwood JR, Pickett MJ. Transfer of Haemophilus vaginalis Gardner and Dukes to a New Genus, Gardnerella: G. vaginalis (Gardner and Dukes) comb. nov. Int J Syst Bacteriol. 1980;30(1):170-178. https://doi.org/10.1099/00207713-30-1-170.
  4. Silhavy TJ, Kahne D, Walker S. The bacterial cell envelope. Cold Spring Harb Perspect Biol. 2010;2(5):a000414. https://doi.org/10.1101/cshperspect.a000414.
  5. Reyn A, Birch-Andersen A, Lapage SP. An Electron Microscope Study of Thin Sections of Haemophilus Vaginalis (Gardner and Dukes) and Some Possibly Related Species. Can J Microbiol. 1966;12(6):1125-1136. https://doi.org/10.1139/m66-154.
  6. Criswell BS, Stenback WA, Black SH, Gardner HL. Fine structure of Haemophilus vaginalis. J Bacteriol. 1972;109(2):930-932.
  7. Sadhu K, Domingue PA, Chow AW, et al. Gardnerella vaginalis has a gram-positive cell-wall ultrastructure and lacks classical cell-wall lipopolysaccharide. J Med Microbiol. 1989;29(3):229-235. https://doi.org/10.1099/00222615-29-3-229.
  8. Muli FW, Struthers JK, Tarpey PA. Electron microscopy studies on Gardnerella vaginalis grown in conventional and biofilm systems. J Med Microbiol. 1999;48(2):211-213. https://doi.org/10.1099/00222615-48-2-211.
  9. Harwich MD, Jr, Alves JM, Buck GA, et al. Drawing the line between commensal and pathogenic Gardnerella vaginalis through genome analysis and virulence studies. BMC Genomics. 2010;11:375. https://doi.org/10.1186/1471-2164-11-375.
  10. Yeoman CJ, Yildirim S, Thomas SM, et al. Comparative genomics of Gardnerella vaginalis strains reveals substantial differences in metabolic and virulence potential. PLoS One. 2010;5(8):e12411. https://doi.org/10.1371/journal.pone.0012411.
  11. Swidsinski A, Doerffel Y, Loening-Baucke V, et al. Gardnerella biofilm involves females and males and is transmitted sexually. Gynecol Obstet Invest. 2010;70(4):256-263. https://doi.org/10.1159/000314015.
  12. Schwebke JR, Muzny CA, Josey WE. Role of Gardnerella vaginalis in the pathogenesis of bacterial vaginosis: a conceptual model. J Infect Dis. 2014;210(3):338-343. https://doi.org/10.1093/infdis/jiu089.
  13. Hickey RJ, Zhou X, Settles ML, et al. Vaginal microbiota of adolescent girls prior to the onset of menarche resemble those of reproductive-age women. MBio. 2015;6(2). https://doi.org/10.1128/mBio.00097-15.
  14. Shen J, Song N, Williams CJ, et al. Effects of low dose estrogen therapy on the vaginal microbiomes of women with atrophic vaginitis. Sci Rep. 2016;6:24380. https://doi.org/10.1038/srep24380.
  15. El Aila NA, Tency I, Saerens B, et al. Strong correspondence in bacterial loads between the vagina and rectum of pregnant women. Res Microbiol. 2011;162(5):506-513. https://doi.org/10.1016/j.resmic.2011.04.004.
  16. Marrazzo JM, Fiedler TL, Srinivasan S, et al. Extravaginal reservoirs of vaginal bacteria as risk factors for incident bacterial vaginosis. J Infect Dis. 2012;205(10):1580-1588. https://doi.org/10.1093/infdis/jis242.
  17. Graham S, Howes C, Dunsmuir R, Sandoe J. Vertebral osteomyelitis and discitis due to Gardnerella vaginalis. J Med Microbiol. 2009;58(Pt 10):1382-1384. https://doi.org/10.1099/jmm.0.007781-0.
  18. Lagace-Wiens PR, Ng B, Reimer A, et al. Gardnerella vaginalis bacteremia in a previously healthy man: case report and characterization of the isolate. J Clin Microbiol. 2008;46(2):804-806. https://doi.org/10.1128/JCM.01545-07.
  19. Neri P, Salvolini S, Giovannini A, Mariotti C. Retinal vasculitis associated with asymptomatic Gardnerella vaginalis infection: a new clinical entity. Ocul Immunol Inflamm. 2009;17(1):36-40. https://doi.org/10.1080/09273940802491876.
  20. Sivadon-Tardy V, Roux AL, Piriou P, et al. Gardnerella vaginalis acute hip arthritis in a renal transplant recipient. J Clin Microbiol. 2009;47(1):264-265. https://doi.org/10.1128/JCM.01854-08.
  21. Tankovic J, Timinskas A, Janulaitiene M, et al. Gardnerella vaginalis bacteremia associated with severe acute encephalopathy in a young female patient. Anaerobe. 2017;47:132-134. https://doi.org/10.1016/j.anaerobe.2017.05.010.
  22. Stewart L, Sinha S, Madsen PJ, et al. Spinal epidural abscess caused by Gardnerella vaginalis and Prevotella amnii. Infect Dis Clin Pract (Baltim Md). 2018;26(4):237-239. https://doi.org/10.1097/IPC.0000000000000565.
  23. Workowski KA, Bolan GA; Centers for Disease Control and Prevention. Sexually transmitted diseases treatment guidelines, 2015. MMWR Recomm Rep. 2015;64(RR-03):1-137.
  24. Janulaitiene M, Paliulyte V, Grinceviciene S, et al. Prevalence and distribution of Gardnerella vaginalis subgroups in women with and without bacterial vaginosis. BMC Infect Dis. 2017;17(1):394. https://doi.org/10.1186/s12879-017-2501-y.
  25. Schellenberg JJ, Patterson MH, Hill JE. Gardnerella vaginalis diversity and ecology in relation to vaginal symptoms. Res Microbiol. 2017;168(9-10):837-844. https://doi.org/10.1016/j.resmic.2017.02.011.
  26. Muzny CA, Schwebke JR. Biofilms: An Underappreciated Mechanism of Treatment Failure and Recurrence in Vaginal Infections. Clin Infect Dis. 2015;61(4):601-606. https://doi.org/10.1093/cid/civ353.
  27. Hardy L, Cerca N, Jespers V, et al. Bacterial biofilms in the vagina. Res Microbiol. 2017;168(9-10):865-874. https://doi.org/10.1016/j.resmic.2017.02.001.
  28. Piot P, van Dyck E, Peeters M, et al. Biotypes of Gardnerella vaginalis. J Clin Microbiol. 1984;20(4):677-679.
  29. Briselden AM, Hillier SL. Longitudinal study of the biotypes of Gardnerella vaginalis. J Clin Microbiol. 1990;28(12):2761-2764.
  30. Moncla BJ, Pryke KM. Oleate lipase activity in Gardnerella vaginalis and reconsideration of existing biotype schemes. BMC Microbiol. 2009;9:78. https://doi.org/10.1186/1471-2180-9-78.
  31. Benito R, Vazquez JA, Berron S, et al. A modified scheme for biotyping Gardnerella vaginalis. J Med Microbiol. 1986;21(4):357-359. https://doi.org/10.1099/00222615-21-4-357.
  32. Aroutcheva AA, Simoes JA, Behbakht K, Faro S. Gardnerella vaginalis isolated from patients with bacterial vaginosis and from patients with healthy vaginal ecosystems. Clin Infect Dis. 2001;33(7):1022-1027. https://doi.org/10.1086/323030.
  33. Pleckaityte M, Janulaitiene M, Lasickiene R, Zvirbliene A. Genetic and biochemical diversity of Gardnerella vaginalis strains isolated from women with bacterial vaginosis. FEMS Immunol Med Microbiol. 2012;65(1):69-77. https://doi.org/10.1111/j.1574-695X.2012.00940.x.
  34. Edmunds PN. The biochemical, serological and hæmag-glutinating reactions of “Hæmophilus vaginalis”. J Pathol Bacteriol. 1962;83(2):411-422. https://doi.org/10.1002/path.1700830211.
  35. Ison CA, Harvey DG, Tanna A, Easmon CS. Development and evaluation of scheme for serotyping Gardnerella vaginalis. Sex Transm Infect. 1987;63(3):196-201. https://doi.org/10.1136/sti.63.3.196.
  36. Wu SR, Hillier SL, Nath K. Genomic DNA fingerprint analysis of biotype 1 Gardnerella vaginalis from patients with and without bacterial vaginosis. J Clin Microbiol. 1996;34(1):192-195.
  37. Ingianni A, Petruzzelli S, Morandotti G, Pompei R. Genotypic differentiation of Gardnerella vaginalis by amplified ribosomal DNA restriction analysis (ARDRA). FEMS Immunol Med Microbiol. 1997;18(1):61-66. https://doi.org/10.1111/j.1574-695X.1997.tb01028.x.
  38. Ahmed A, Earl J, Retchless A, et al. Comparative genomic analyses of 17 clinical isolates of Gardnerella vaginalis provide evidence of multiple genetically isolated clades consistent with subspeciation into genovars. J Bacteriol. 2012;194(15):3922-3937. https://doi.org/10.1128/JB.00056-12.
  39. Balashov SV, Mordechai E, Adelson ME, Gygax SE. Identification, quantification and subtyping of Gardnerella vaginalis in noncultured clinical vaginal samples by quantitative PCR. J Med Microbiol. 2014;63(Pt 2):162-175. https://doi.org/10.1099/jmm.0.066407-0.
  40. Srinivasan S, Hoffman NG, Morgan MT, et al. Bacterial communities in women with bacterial vaginosis: high resolution phylogenetic analyses reveal relationships of microbiota to clinical criteria. PLoS One. 2012;7(6):e37818. https://doi.org/10.1371/journal.pone.0037818.
  41. Lin L, Song J, Kimber N, et al. The role of bacterial vaginosis in infection after major gynecologic surgery. Infect Dis Obstet Gynecol. 1999;7(3):169-174. https://doi.org/10.1155/S1064744999000277.
  42. Chen Y, Han X, Guo P, et al. Bacteremia Caused by Gardnerella Vaginalis in a Cesarean Section Patient. Clin Lab. 2018;64(3):379-382. https://doi.org/10.7754/Clin.Lab.2017.171035.
  43. Bretelle F, Rozenberg P, Pascal A, et al. High Atopobium vaginae and Gardnerella vaginalis vaginal loads are associated with preterm birth. Clin Infect Dis. 2015;60(6):860-867. https://doi.org/10.1093/cid/ciu966.
  44. Dingens AS, Fairfortune TS, Reed S, Mitchell C. Bacterial vaginosis and adverse outcomes among full-term infants: a cohort study. BMC Pregnancy Childbirth. 2016;16(1):278. https://doi.org/10.1186/s12884-016-1073-y.
  45. Brown RG, Marchesi JR, Lee YS, et al. Vaginal dysbiosis increases risk of preterm fetal membrane rupture, neonatal sepsis and is exacerbated by erythromycin. BMC Med. 2018;16(1):9. https://doi.org/10.1186/s12916-017-0999-x.
  46. Romero R, Hassan SS, Gajer P, et al. The vaginal microbiota of pregnant women who subsequently have spontaneous preterm labor and delivery and those with a normal delivery at term. Microbiome. 2014;2:18. https://doi.org/10.1186/2049-2618-2-18.
  47. Eastment MC, McClelland RS. Vaginal microbiota and susceptibility to HIV. AIDS. 2018;32(6):687-698. https://doi.org/10.1097/QAD.0000000000001768.
  48. Reis Machado J, da Silva MV, Cavellani CL, et al. Mucosal immunity in the female genital tract, HIV/AIDS. Biomed Res Int. 2014;2014:350195. https://doi.org/10.1155/2014/350195.
  49. Hashemi FB, Ghassemi M, Roebuck KA, Spear GT. Activation of human immunodeficiency virus type 1 expression by Gardnerella vaginalis. J Infect Dis. 1999;179(4):924-930. https://doi.org/10.1086/314674.
  50. Machado A, Cerca N. Influence of Biofilm Formation by Gardnerella vaginalis and Other Anaerobes on Bacterial Vaginosis. J Infect Dis. 2015;212(12):1856-1861. https://doi.org/10.1093/infdis/jiv338.
  51. Datcu R, Gesink D, Mulvad G, et al. Vaginal microbiome in women from Greenland assessed by microscopy and quantitative PCR. BMC Infect Dis. 2013;13:480. https://doi.org/10.1186/1471-2334-13-480.
  52. Gelber SE, Aguilar JL, Lewis KL, Ratner AJ. Functional and phylogenetic characterization of Vaginolysin, the human-specific cytolysin from Gardnerella vaginalis. J Bacteriol. 2008;190(11):3896-3903. https://doi.org/10.1128/JB.01965-07.
  53. Santiago GL, Deschaght P, El Aila N, et al. Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidase. Am J Obstet Gynecol. 2011;204(5):450 e451-457. https://doi.org/10.1016/j.ajog.2010.12.061.
  54. Amith SR, Jayanth P, Franchuk S, et al. Dependence of pathogen molecule-induced toll-like receptor activation and cell function on Neu1 sialidase. Glycoconj J. 2009;26(9):1197-1212. https://doi.org/10.1007/s10719-009-9239-8.
  55. Moncla BJ, Chappell CA, Debo BM, Meyn LA. The Effects of Hormones and Vaginal Microflora on the Glycome of the Female Genital Tract: Cervical-Vaginal Fluid. PLoS One. 2016;11(7):e0158687. https://doi.org/10.1371/journal.pone.0158687.
  56. Lewis WG, Robinson LS, Gilbert NM, et al. Degradation, foraging, and depletion of mucus sialoglycans by the vagina-adapted Actinobacterium Gardnerella vaginalis. J Biol Chem. 2013;288(17):12067-12079. https://doi.org/10.1074/jbc.M113.453654.
  57. Briselden AM, Moncla BJ, Stevens CE, Hillier SL. Sialidases (neuraminidases) in bacterial vaginosis and bacterial vaginosis-associated microflora. J Clin Microbiol. 1992;30(3):663-666.
  58. Schellenberg JJ, Paramel Jayaprakash T, Withana Gamage N, et al. Gardnerella vaginalis Subgroups Defined by cpn60 Sequencing and Sialidase Activity in Isolates from Canada, Belgium and Kenya. PLoS One. 2016;11(1):e0146510. https://doi.org/10.1371/journal.pone.0146510.
  59. Patterson JL, Stull-Lane A, Girerd PH, Jefferson KK. Analysis of adherence, biofilm formation and cytotoxicity suggests a greater virulence potential of Gardnerella vaginalis relative to other bacterial-vaginosis-associated anaerobes. Microbiology. 2010;156(Pt 2):392-399. https://doi.org/10.1099/mic.0.034280-0.
  60. Alves P, Castro J, Sousa C, et al. Gardnerella vaginalis outcompetes 29 other bacterial species isolated from patients with bacterial vaginosis, using in an in vitro biofilm formation model. J Infect Dis. 2014;210(4):593-596. https://doi.org/10.1093/infdis/jiu131.
  61. Patterson JL, Girerd PH, Karjane NW, Jefferson KK. Effect of biofilm phenotype on resistance of Gardnerella vaginalis to hydrogen peroxide and lactic acid. Am J Obstet Gynecol. 2007;197(2):170.e171-177. https://doi.org/10.1016/j.ajog.2007.02.027.
  62. Verstraelen H, Swidsinski A. The biofilm in bacterial vaginosis: implications for epidemiology, diagnosis and treatment. Curr Opin Infect Dis. 2013;26(1):86-89. https://doi.org/10.1097/QCO.0b013e32835c20cd.
  63. Schwebke JR, Flynn MS, Rivers CA. Prevalence of Gardnerella vaginalis among women with lactobacillus-predominant vaginal flora. Sex Transm Infect. 2014;90(1):61-63. https://doi.org/10.1136/sextrans-2013-051232.

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