New approaches to the estimation of endometrial dysfunction

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Abstract

Introduction. The application of modern methods for assessing the morphofunctional state of the endometrium to verify and study the expression of sex steroid hormones, proinflammatory markers and markers of angiogenesis using confocal laser scanning microscopy will allow an objective study of the role of studied markers in the pathogenesis of the endometrial dysfunction.

The aim of the study was to evaluate the expression of ER and PR receptors in endometrium in patients with endometrial dysfunction.

Material and methods. Endometrial biopsy specimens obtained with the aid of a pile-biopsy or a scraping from the uterine cavity are used to conduct the method of immunofluorescent confocal laser scanning microscopy. It is possible to use both cryostat material and paraffin blocks to provide the immunohistochemical analysis. Monoclonal antibodies to ER (1 : 60, Dako, Denmark) and PR (1 : 50, Dako, Denmark) are used as primary antibodies, antibodies conjugated with fluorochrome Alexa Fluor 647 (1 : 1000, Abcam, England) are used as secondary antibodies). Hoechst 33258 (Sigma, USA) is used for staining of cell nuclei.

Results. A method of confocal laser scanning microscopy makes it possible to conduct qualitative and quantitative evaluation of the studied markers in different structures of the endometrium.

About the authors

Edvard K. Aylamazyan

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Author for correspondence.
Email: iagmail@ott.ru

chief, academician, professor

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Gulrukhsor Kh. Tolibova

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: gulyatolibova@mail.ru

PhD, MD

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Tatyana G. Tral

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: TTG2008@bk.ru

PhD, MD, Pathologist, Head of Pathologist Laboratory, Department of Pathomorphology

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Igor U. Kogan

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: ikogan@mail.ru

PhD, AM RAM, Scientific secretary

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Mariya I. Yarmolinskaya

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: m.yarmolinskaya@gmail.com

professor of Russian Academy of Sciences, leading research assistant of department of endocrinology of reproduction, Doctor of medical sciences, head of center “Diagnostics and treatment of endometriosis”

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Anna A. Tsipurdeeva

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: tsypurdeeva@mail.ru

MD, Head of Department of Operative Gynecology

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Valeriia R. Rodichkina

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: rolerus@mail.ru

junior research scientist of the Pathomorphology department

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Igor M. Kvetnoy

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: igor.kvetnoy@yandex.ru

MD, professor. Head of the Pathomorphology Department

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

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Copyright (c) 2017 Aylamazyan E.K., Tolibova G.K., Tral T.G., Kogan I.U., Yarmolinskaya M.I., Tsipurdeeva A.A., Rodichkina V.R., Kvetnoy I.M.

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