The vaginal microbiota in the first trimester of pregnancy in women having history of pregnancy loss

Cover Page


Cite item

Full Text

Abstract

Introduction. Abnormal vaginal microflora in the first trimester of pregnancy is a risk factor for miscarriage. Opportunistic microorganisms predominate among microorganisms causing microflora disturbance. Aim. The aim of the study was to evaluate the vaginal microflora in the first trimesterф

Introduction. Abnormal vaginal microflora in the first trimester of pregnancy is a risk factor for miscarriage. Opportunistic microorganisms predominate among microorganisms causing microflora disturbance. 

Aim. The aim of the study was to evaluate the vaginal microflora in the first trimester of pregnancy in women having history of pregnancy loss. 

Matherials and Methods. Real-time polymerase chain reaction, microscopic and cultural methods were used to examine 60 pregnant women having history of pregnancy loss and 10 pregnant women with uncomplicated obstetric history. 

Results. Disorders of the vaginal microflora were observed only in women having history of pregnancy loss. The lactoflora in both groups was shown to consist most frequently of 3 Lactobacillus species: Lactobacillus crispatus, L. jensenii, and L. iners. However, the species L. iners were detected only in the group of women with history of pregnancy loss. Non-Lactobacillus microbiota was significantly more often observed in pregnant women having history of pregnancy loss. Facultative and obligate anaerobes were detected in both groups. The concentration of Ureaplasma spp. and Mycoplasma hominis in women in both groups had no significant differences. 

Conclusions. The results of this study indicate that abnormal microbiota is more often observed in women having history of pregnancy loss.

of pregnancy in women having history of pregnancy loss. Matherials and Methods. Real-time polymerase chain reaction, microscopic and cultural methods were used to examine 60 pregnant women having history of pregnancy loss and 10 pregnant women with uncomplicated obstetric history. Results. Disorders of the vaginal microflora were observed only in women having history of pregnancy loss. The lactoflora in both groups was shown to consist most frequently of 3 Lactobacillus species: Lactobacillus crispatus, L. jensenii, and L. iners. However, the species L. iners were detected only in the group of women with history of pregnancy loss. Non-Lactobacillus microbiota was significantly more often observed in pregnant women having history of pregnancy loss. Facultative and obligate anaerobes were detected in both groups. The concentration of Ureaplasma spp. and Mycoplasma hominis in women in both groups had no significant differences. Conclusions. The results of this study indicate that abnormal microbiota is more often observed in women having history of pregnancy loss.

About the authors

Anna A. Siniakova

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: savitcheva@mail.ru
post-graduate student Russian Federation

Elena V. Shipitsyna

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: savitcheva@mail.ru
PhD, Senior Researcher, Laboratory of Microbiology Russian Federation

Elena V. Rybina

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: savitcheva@mail.ru
Bacteriologist, Laboratory of Microbiology Russian Federation

Olga V. Budilovskaya

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: savitcheva@mail.ru
Researcher, Laboratory of Microbiology Russian Federation

Tamara A. Pluzhnikova

Center of prophylaxis and treatment of miscarriage, Birth House No 1

Email: savitcheva@mail.ru
PhD, chief of Center Russian Federation

Viacheslav M. Bolotskikh

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: savitcheva@mail.ru
MD, deputy director for Medical work Russian Federation

Alevtina M. Savicheva

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Author for correspondence.
Email: savitcheva@mail.ru
MD, Professor, Head of Laboratory of Microbiology Russian Federation

References

  1. Petricevic L, Domig K, Nierscher FJ, et al. Characterisation of the vaginal Lactobacillus microbiota associated with preterm delivery. Sci Rep. 2014;4:51362. doi: 10.1038/srep05136.
  2. Орлова B.C., Набережнев Ю.И. Нормоценоз влагалища у женщин репродуктивного возраста, механизмы его регуляции и дисбиотические варианты // Российский вестник акушера-гинеколога. — 2007. — № 4. — С. 36–39. [Orlova VS, Naberezhnev YuI. Vaginal normocenosis in reproductive-aged females, mechanisms of its regulation and dysbiotic variants. Rossijskij vestnikakushera-ginekologa. 2007;4:36-39. (In Russ).]
  3. Берлев И.В., Кира Е.Ф. Роль условно-патогенной микрофлоры в развитии невынашивания беременности у женщин с нарушением микробиоценоза влагалища // Журнал акушерства и женских болезней. — 2002. — Т. 51. — № 1. — С. 33–37. [Berlev IV, Kira EF. Rol’ uslovno patogennoj mikroflory v razvitii nevynashivanija beremennosti u zhenshhin s narusheniemmikrobiocenoza vlagalishha. Journal of Obstetrics and Women’s Diseases. 2002;51(1):33-37. (In Russ).]
  4. French JI, McGregor JA, Draper D, et al. Gestational bleeding, bacterial vaginosis, and common reproductive tract infections: risk for preterm birth and benefit of treatment. Obstet Gynecol.1999;93(5):715-724.
  5. Leitich H, Bodner-Adler B, Brunbauer M, et al. Bacterial vaginosis as a risk factor for preterm delivery: a meta-analysis. Am J Obstet Gyncol. 2003;189(1):139-147.
  6. Paneth NS. The problem of low birth weight. Future Child. 1995;5(1):19-34.
  7. Donders GG, Van Calsteren K, Bellen G, et al. Predictive value for preterm birth of abnormal vaginal flora, bacterial vaginosis and aerobic vaginitis during the first trimester of pregnancy. BJOG.2009;116(10):1315-24. doi: 10.1111/j.1471-0528.2009.02237.x.
  8. Horowitz BJ, Mardh PA, Hagy E, et al. Vaginal lactobacillosis. Am J Obstet Gyncol. 1994;170(3):857-61.
  9. Burton JP, Cadieux PA, Reid G. Improved understanding of the bacterial vaginal microbiota of women before and after probiotic instillation. Appl Environ Microbiol. 2003;69(1):97-101.
  10. Antonio MA , Hawes SE, Hillier SL. The identification of vaginal Lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species. J Infect Dis.1999;180:1950-1956.
  11. Macklaim JM, Gloor GB, Anukam KC, et al. At the crossroads of vaginal health and disease, the genome sequence of Lactobacillus iners AB-1. Proc Natl Acad Sci USA. 2011;108(1):4688-4695 doi: 10.1073/pnas.1000086107.
  12. Antonio MA, Rabe LK, Hillier SL.Colonization of the rectum by Lactobacillus species and decreased of bacterial vaginosis. J Infect Dis. 2005;192:394-398. doi: 10.1086/430926.
  13. Rampersaud R, Planet PJ, Randis TM, et al. Inerolysin, a cholesterol-dependent cytolysin produced by Lactobacillus iners. J Bacteriol. 2011;193(5):1034-1041.
  14. Nelson Deborah B, Bellamy Scarlett, Nachamkin Irving, et al. First trimester bacterial vaginosis, individual microorganism levels, and risk of second trimester pregnancy loss among urban women. Fertility and Sterility. 2007;88(5):1396-403. doi: 10.1016/j.fertnstert.2007.01.035.
  15. Сорокина О.В., Шипицына Е.В, Болотских В.М., и др. Оценка микробиоценоза влагалища у женщин с преждевременным излитием околоплодных вод методом полимеразной цепной реакции в реальном времени // Журнал акушерства и женских болезней. — 2012. — № 2. — С. 57–64. [Sorokina OV, Shipitsina EV, Bolotskikh VM, et al. Evaluation of vaginal microbiota in women with premature rupture of membrane and onset of labor after 24 hours using real-time polymerase chain reaction. Journal of Obstetrics and Women’s Diseases. 2012;2:57-64 (In Russ).]

Copyright (c) 2016 Siniakova A.A., Shipitsyna E.V., Rybina E.V., Budilovskaya O.V., Pluzhnikova T.A., Bolotskikh V.M., Savicheva A.M.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies