Comparison of the variability and nitrogen-fixing activity of rhizobia strains isolated from Trifolium Hybridum L. and Galegaorientalis Lam. nodules at different stages of plant vegetation
- Authors: Baymiev A.K.1, Koryakov I.S.1, Akimova E.S.1, Vladimirova A.A.1, Matniyazov R.T.1, Baymiev A.K.1
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Affiliations:
- Institute of Biochemistry and Genetics — Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences
- Issue: Vol 21, No 3 (2023)
- Pages: 207-217
- Section: Genetic basis of ecosystems evolution
- URL: https://journals.rcsi.science/ecolgenet/article/view/148877
- DOI: https://doi.org/10.17816/ecogen313071
- ID: 148877
Cite item
Abstract
BACKGROUND: The beginning of the life cycle of a leguminous plant in its natural habitat is usually associated with interaction with nodule bacteria in order to form a nitrogen-fixing symbiosis. In a short period of time, a plant needs to “choose” suitable microsymbionts for itself. Since a wide variety of rhizobial strains is formed in the rhizosphere of legumes, the choice made by the macrosymbiont will further influence its productivity.
AIM: The purpose of our work was to compare the principles of selection by different plants of their microsymbionts at different stages of plant development.
MATERIALS AND METHODS: Nodule bacteria Trifolium hybridum L. and Galegaorientalis Lam. were taken into the study. Their genetic diversity was studied by the RAPD method, a phylogenetic analysis of bacteria and their symbiotic nodC and nifH genes was carried out, and their nitrogen-fixing activity was assessed.
RESULTS: It was found that the rhizobia that form nodules on the roots of the studied leguminous plants at different stages of their vegetation have certain patterns. It was found that the highest polymorphism and specific nitrogen-fixing activity are characteristic of bacteria obtained from nodules formed at the initial stage of vegetation.
CONCLUSIONS: We assume that the plasticity of the rhizobia genome gives the host plant the ability to more flexibly adjust its nitrogen-fixing apparatus to changes in growing conditions.
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##article.viewOnOriginalSite##About the authors
Andrei Kh. Baymiev
Institute of Biochemistry and Genetics — Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences
Email: baymiev@anrb.ru
ORCID iD: 0000-0001-6637-9365
SPIN-code: 1919-5236
Dr. Sci. (Med.), leading research associate, Laboratory of plant and microbial bioengineering
Russian Federation, UfaIgor S. Koryakov
Institute of Biochemistry and Genetics — Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences
Author for correspondence.
Email: koryakov_igor@mail.ru
SPIN-code: 5586-7123
postgraduate student, Laboratory of plant and microbial bioengineering
Russian Federation, UfaEkaterina S. Akimova
Institute of Biochemistry and Genetics — Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences
Email: iv.katerina-bio@yandex.ru
SPIN-code: 6595-2452
Cand. Sci. (Biol.), research associate, Laboratory of plant and microbial bioengineering
Russian Federation, UfaAnastasiya A. Vladimirova
Institute of Biochemistry and Genetics — Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences
Email: vladimirovaw@bk.ru
SPIN-code: 2059-9396
Cand. Sci. (Biol.), research associate, Laboratory of plant and microbial bioengineering
Russian Federation, UfaRustam T. Matniyazov
Institute of Biochemistry and Genetics — Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences
Email: rmat@mail.ru
SPIN-code: 6798-7913
Cand. Sci. (Biol.), research associate, Laboratory of plant and microbial bioengineering
Russian Federation, UfaAlexei Kh. Baymiev
Institute of Biochemistry and Genetics — Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences
Email: baymiev@mail.ru
ORCID iD: 0000-0003-0606-6740
SPIN-code: 3771-4063
Dr. Sci. (Biol.), head of Laboratory of plant and microbial bioengineering
Russian Federation, UfaReferences
- Spoerke JA, Wilkinson HH, Parker MA. Nonrandom genotypic associations in a legumе — Bradyrhizobium mutualism. Evolution. 1996;50(1):146–154. doi: 10.1111/j.1558-5646.1996.tb04481.x
- Paffetti D, Daguin F, Fancelli S, et al. Influence of plant genotype on the selection of nodulating Sinorhizobium meliloti strains by Medicago sativa. Antonie van Leeuwenhoek. 1998;73:3–8. doi: 10.1023/a:1000591719287
- Carelli М, Gnocchi S, Fancelli S, et al. Genetic diversity and dynamics of Sinorhizobium meliloti populations nodulating different alfalfa cultivars in Italian soils. Appl Environ Microbiol. 2000;66(11):4785–4789. doi: 10.1128/AEM.66.11.4785-4789.2000
- Andronov EE, Terefework Z, Roumiantseva ML, et al. Symbiotic and genetic diversity of Rhizobium galegae isolates collected from the Galegaorientalis gene center in the Caucasus. Appl Environ Microbiol. 2003;69(2):1067–1074. doi: 10.1128/AEM.69.2.1067-1074.2003
- Ling J, Wang H, Wu P, et al. Plant nodulation inducers enhance horizontal gene transfer of Azorhizobium caulinodans symbiosis island. PNAS. 2016;113(48):13875–13880. doi: 10.1073/pnas.16151211
- Vavilov PP, Khaig KhA. Vozdelyvanie i ispol’zovanie kozlyatnika vostochnogo. Leningrad: Kolos, 1982. 72 p. (In Russ.)
- Nadezhkin SN, Kuznetsov IYu. Kozlyatnik vostochnyi na korm i semena. Ufa: BGAU, 2008. 143 p. (In Russ.)
- Romero D, Martinez-Salazar J, Girard L, et al. Discrete amplifiable regions (amplicons) in the symbiotic plasmid of Rhizobium etli CFN42. J Bacteriol. 1995;177(4):973–980. doi: 10.1128/jb.177.4.973-980.1995
- Baymiev AnKh, Ptitsyn KG, Baimiev AlKh. Influence of the introduction of Caragana arborescens on the composition of its root-nodule bacteria. Microbiology. 2010;79:115–120. (In Russ.) doi: 10.1134/S0026261710010157
- Williams JG, Kubelik AR, Livak KJ, et al. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990;18(22):6531–6535. doi: 10.1093/nar/18.22.6531
- Laguerre G, Mavingui P, Allard MR, et al. Typing of rhizobia by PCR DNA fingerprinting and PCR-restriction fragment length polymorphism analysis of chromosomal and symbiotic gene regions: application to Rhizobium leguminosarum and its different biovars. J Appl Environ Microbiol. 1996;62(6):2029–2036. doi: 10.1128/aem.62.6.2029-2036.1996
- Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 1991;173(2):697–703. doi: 10.1128/jb.173.2.697-703.1991
- Baymiev AnKh, Ivanova ES, Gumenko RS, et al. Analysis of symbiotic genes of leguminous root nodule bacteria grown in the Southern Urals. Russian Journal of Genetics. 2015;51:1172–1180. (In Russ.) doi: 10.1134/S1022795415110034
- Umarov MM. Assotsiativnaya azotfiksatsiya. Moscow: MGU Publ., 1989. 136 p. (In Russ.)
- Śliwka J, Sobkowiak S, Lebecka R, et al. Mating type, virulence, aggressiveness and metalaxyl resistance of isolates of Phytophthora infestans in Poland. Potato Res. 2006;49:155–166. doi: 10.1007/s11540-006-9013-2
- Osterman J, Chizhevskaja EP, Andronov EE, et al. Galegaorientalis is more diverse than Galega officinalis in Caucasus-whole-genome AFLP analysis and phylogenetics of symbiosis-related genes. Mol Ecol. 2011;20(22):4808–4821. doi: 10.1111/j.1365-294X.2011.05291.x
- Denison RF. Legume sanctions and the evolution of symbiotic cooperation by rhizobia. American Naturalist. 2000;156(6):567–576. doi: 10.1086/316994
- Kiers TE, Rousseau RA, West SA, Denison RF. Host sanctions and the legume-rhizobium mutualism. Nature. 2003;425:78–81. doi: 10.1038/nature01931