Study of acetylated histone h3k9 – an active chromatin mark – in chromosomes from adult and fetal human lymphocytes

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Abstract

Background: Incorrect epigenetic modifications of the human genome may result in epigenetic disorders, thus, highlighting the necessity of studying chromosome epigenetic patterns in human development.

Aim of the study: A comparative analysis of acetylated histone H3K9 (AcH3K9) patterns in human metaphase chromosomes from the lymphocytes of adults and fetuses.

            Materials and methods: The immunocytochemical detection of AcH3K9 in the metaphase chromosomes from PHA-stimulated peripheral lymphocytes of 13 adults and cord blood lymphocytes of 10 fetuses at 20-22 weeks of gestation.

Results: Both in the chromosomes of the adults and the fetuses, AcH3K9 accumulated in the R- and T-, but not G-bands and avoided the regions of pericentromeric heterochromatin of the chromosomes 1, 9 and 16. When comparing the adult and the fetal chromosomes, different levels of AcH3K9 were revealed in a few bands: 2q31, 5p13, 5p15 and 16p13 had higher level of Н3К9 acetylation in adults, in contrast to 9q13 which was hyperacetylated in fetuses.

Conclusion: The АсН3К9 distribution in metaphase chromosomes is band-specific and is similar between the adults and the fetuses, excluding a few bands with different acetylation levels.

About the authors

Olga A. Efimova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology

Author for correspondence.
Email: efimova_o82@mail.ru
ORCID iD: 0000-0003-4495-0983
SPIN-code: 6959-5014
Scopus Author ID: 14013324600

PhD, researcher, laboratory for prenatal diagnosis of congenital and inherited diseases

Russian Federation, 3, Mendeleevskaya line, St. Petersburg, 199034

Anna A. Pendina

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Сenter for Medical Genetics

Email: pendina@mail.ru
SPIN-code: 3123-2133

PhD, researcher, laboratory for prenatal diagnosis of congenital and inherited diseases

Russian Federation, 3, Mendeleevskaya line, St. Petersburg, 199034; 5, Tobolskaya ul., St. Petersburg, 194044

Yuliia G. Lezhnina

Center for Medical Genetics

Email: avesfly@mail.ru

biologist

Russian Federation, 5, Tobolskaya ul., St. Petersburg, 194044

Andrei V. Tikhonov

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Center for Medical Genetics

Email: tixonov5790@gmail.com
SPIN-code: 3170-2629

PhD, researcher, laboratory for prenatal diagnosis of congenital and inherited diseases

Russian Federation, 3, Mendeleevskaya line, St. Petersburg, 199034; 5, Tobolskaya ul., St. Petersburg, 194044

Olga G. Chiryaeva

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: chiryaeva@mail.ru
SPIN-code: 4027-4908

PhD, researcher, laboratory for prenatal diagnosis of congenital and inherited diseases

Russian Federation, 3, Mendeleevskaya line, St. Petersburg, 199034

Lyubov I. Petrova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: petrovaluba@mail.ru
SPIN-code: 8599-6886

laboratory geneticist, laboratory for prenatal diagnosis of congenital and inherited diseases

Russian Federation, 3, Mendeleevskaya line, St. Petersburg, 199034

Vera S. Dudkina

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: dudkinavs@mail.ru
SPIN-code: 6286-7287

laboratory geneticist, laboratory for prenatal diagnosis of congenital and inherited diseases

Russian Federation, 3, Mendeleevskaya line, St. Petersburg, 199034

Alla S. Koltsova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Saint Petersburg State University

Email: rosenrot15@yandex.ru
SPIN-code: 3038-4096

assistant researcher, laboratory for prenatal diagnosis of congenital and inherited diseases

Russian Federation, 3, Mendeleevskaya line, St. Petersburg, 199034; 7/9, Universitetskaya emb., St. Petersburg, 199034

Mikhail I. Krapivin

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Saint Petersburg State University

Email: krapivin-mihail@mail.ru
SPIN-code: 4989-1932

assistant researcher, laboratory for prenatal diagnosis of congenital and inherited diseases

Russian Federation, 3, Mendeleevskaya line, St. Petersburg, 199034; 7/9, Universitetskaya emb., St. Petersburg, 199034

Anastasiia V. Petrovskaia-Kaminskaia

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Saint Petersburg State University

Email: tonx2012@yandex.ru

assistant researcher, laboratory for prenatal diagnosis of congenital and inherited diseases

Russian Federation, 3, Mendeleevskaya line, St. Petersburg, 199034; 7/9, Universitetskaya emb., St. Petersburg, 199034

Olga E. Talantova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: olga_talantova@mail.ru

MD, researcher, laboratory for prenatal diagnosis of congenital and inherited diseases

Russian Federation, 3, Mendeleevskaya line, St. Petersburg, 199034

Tatiana V. Kuznetzova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: tkuznetzova@mail.ru
SPIN-code: 1000-7522

PhD, researcher, laboratory for prenatal diagnosis of congenital and inherited diseases

Russian Federation, 3, Mendeleevskaya line, St. Petersburg, 199034

Vladislav S. Baranov

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Saint Petersburg State University

Email: baranov@vb2475.spb.edu
SPIN-code: 9196-7297

MD, Professor, laboratory head, laboratory for prenatal diagnosis of congenital and inherited diseases

Russian Federation, 3, Mendeleevskaya line, St. Petersburg, 199034; 7/9, Universitetskaya emb., St. Petersburg, 199034

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Copyright (c) 2019 Efimova O.A., Pendina A.A., Lezhnina Y.G., Tikhonov A.V., Chiryaeva O.G., Petrova L.I., Dudkina V.S., Koltsova A.S., Krapivin M.I., Petrovskaia-Kaminskaia A.V., Talantova O.E., Kuznetzova T.V., Baranov V.S.

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