Effect of DNA methylation on the interaction with tetrahydrocortisol–apolipoprotein A-I complex

Аннотация

The effect of GCNGC site methylation on the DNA binding to tetrahydrocortisol–apolipoprotein A-I complex was shown by atomic force microscopy and enzymatic analysis. Methylation of GCNGC sites alters the mechanism of interaction of tetrahydrocortisol–apolipoprotein A-I complex with DNA and leads to the aggregation of the DNA-protein complexes. It was assumed that the involvement of complexes of steroid hormones with apolipoprotein A-I in the process of chromatin restructuring is associated with the processes of DNA methylation/demethylation.