Флуоресцентная время-разрешённая микроскопия в иммуноонкологии: мониторинг гетерогенности опухоли, клеточной гибели и динамики иммунного ответа

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Аннотация

Флуоресцентная микроскопия с временным разрешением (FLIM) активно развивается на протяжении последних двух десятилетий и играет значительную роль в биомедицинских исследованиях. Современные достижения в создании флуоресцентных зондов существенно расширили потенциал данного метода. Учитывая, что время жизни флуоресценции чувствительно к микросреде и молекулярным изменениям, FLIM представляет собой перспективный инструмент для выявления патологических состояний, включая онкологические заболевания. Эта технология позволяет наблюдать структуру опухоли и отслеживать динамические процессы в режиме реального времени, что дает возможность исследователям с поразительной точностью исследовать живые раковые клетки и их микроокружение. Более того, данный метод открывает новые возможности для мониторинга эффективности противоопухолевой терапии. Особый интерес представляет применение FLIM в разработке и оценке эффективности иммунотерапевтических стратегий. В контексте терапии солидных опухолей ключевое значение имеют данные о метаболизме и гетерогенности опухоли, механизмах клеточной гибели, а также о динамике иммунного ответа после терапевтического воздействия.

В настоящем обзоре на основании анализа современных научных данных мы обосновываем целесообразность использования FLIM в качестве важного инструмента в исследованиях, направленных на совершенствование методов иммунотерапии рака.

Об авторах

Алина Ринатовна Хузина

Научно-технологический университет «Сириус»

Email: huzinaar@gmail.com
ORCID iD: 0009-0007-9873-7471
Россия, Сириус

Виктория Дмитриевна Турубанова

Научно-технологический университет «Сириус»; Национальный исследовательский Нижегородский государственный университет имени Н.И. Лобачевского

Автор, ответственный за переписку.
Email: turubanova@neuro.nnov.ru
ORCID iD: 0000-0002-4648-0738
SPIN-код: 8262-6560

канд. биол. наук

Россия, Сириус; Нижний Новгород

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