STUDY OF BAICALIN HYDROLYSIS KINETICS IN THE PROCESS OF ITS EXTRACTION FROM SCUTELLARIA BAICALENSIS GEORGI ROOTS


如何引用文章

全文:

详细

The aim of this study was to investigate the kinetics of baicalin hydrolysis in the process of its extraction from Scutellaria baicalensis Georgi roots.Materials and methods. For the studies, Scutellaria baicalensis Georgi roots with a particle range of 0.1-0.5 mm were used. The method of extraction was a simple maceration during a specified period of time, the ratio of plant raw material : extractant was 1:10 w/v at the temperature of 24±1°C. Baicalin and baicalein contents were analyzed by reverse phase high performance liquid chromatography (RP HPLC) at the analytical wavelength of 275 nm. The extractant was a water solution of ethanol 26, 43, 59, 72, 81, 97±1% v/v. The time of the extraction was from 1 to 24 hours.Results. The experimental points of dependency of baicalin concentration in the extract on the time of extraction for ethanol solutions with a concentration of 43 and 72% v/v are closely approximated by a linear equation in coordinates lnC=f(t). The value of determination coefficient is more than R²˃0,99. Half lifetime for baicalin has been calculated: for ethanol with the concentration of 43% v/v it is 4.3±0.7 hours, and for ethanol with the concentration of 72% v/v it is 42.3±1.8 hours.Conclusion. Baicalin hydrolysis kinetics in the process of its extraction from Scutellaria baicalensis Georgi roots with 43 and 72% v/v ethanol concentration. has been studied. It has been established that the process of baicalin hydrolysis is well described by the first order kinetic equation. The constants of baicalin hydrolysis during its extraction from Scutelaria baicalensis roots with ethanol having different concentrations have been calculated. Recommendations on technology optimization for baicalin or baicalein extraction from Scutellaria baicalensis Georgi roots have been given.

作者简介

N. Boyko

Belgorod National Research University

Email: boykoniknik@gmail.com

D. Pisarev

Belgorod National Research University

Email: pisarev@bsu.edu.ru

E. Zhilyakova

Belgorod National Research University

Email: ezhilyakova@bsu.edu.ru

A. Maljutina

Belgorod National Research University

Email: malyutina_a@bsu.edu.ru

O. Novikov

Peoples’ Friendship University of Russia

Email: ole9222@yandex.ru

M. Bocharnikova

Belgorod National Research University

Email: bocharnikova_m@bsu.edu.ru

参考

  1. Попова Н.В., Литвиненко В.И., Куцанян А.С. Лекарственные растения мировой флоры: энциклопед. справочник. - Харьков: Діса плюс, 2016. - 540 с.
  2. WHO monographs on selected medicinal plants. Vol. 3. - Geneva: World Health Organization, 2007. - 390 p.
  3. Li C., Lin G., Zuo Z. Pharmacological effects and pharmacokinetics properties of Radix Scutellariae and its bioactive flavones // Biopharmaceutics & Drug Disposition. - 2011. - Vol. 32(8). - 427-445. doi: 10.1002/bdd.771.
  4. Bie B. Baicalein, a Natural Anti-Cancer Compound, Alters MicroRNA Expression Profiles in Bel-7402 Human Hepatocellular Carcinoma Cells / B. Bie, J. Sun, J. Li, Y. Guo, W. Jiang, C. Huang, J. Yang, Z. Li // Cell Physiol Biochem. - 2017. - Vol. 41(4). - P. 1519-1531. doi: 10.1159/000470815. Epub 2017 Mar 24.
  5. Sowndhararajan K. Neuroprotective and Cognitive Enhancement Potentials of Baicalin: A Review / K. Sowndhararajan, P. Deepa, M. Kim, S.J. Park, S. Kim // Brain Sciences. - 2018. - Vol. 8(6). - P. 104. DOI: https://doi.org/10.3390/brainsci8060104
  6. Shi H. Baicalin from Scutellaria baicalensis blocks respiratory syncytial virus (RSV) infection and reduces inflammatory cell infiltration and lung injury in mice / H. Shi, K. Ren, B. Lv, W. Zhang, Y. Zhao, R.X. Tan, E. Li // Scientific Reports. - 2016. - N 6. - P. 35851. doi: 10.1038/srep35851
  7. Zhang X.W. Protective effects of the aqueous extract of Scutellaria baicalensis against acrolein-induced oxidative stress in cultured human umbilical vein endothelial cells / X.W. Zhang, W.F. Li, W.W. Li, K.H. Ren, C.M. Fan, Y.Y. Chen, Y.L. Shen // Pharm Biol. - 2011. - N 49. - P. 256-261. DOI: https://doi.org/10. 3109/13880209.2010.501803
  8. Gasiorowski K. Flavones from root of Scutellaria baicalensis Georgi: drugs of the future in neurodegeneration? / K. Gasiorowski, E. Lamer-Zarawska, J. Leszek, K. Parvathaneni, B.B. Yendluri, Z. Blach-Olszewska, G. Aliev // CNS Neurol Disord Drug Targets. - 2011. - N 10. - P. 184-191. DOI: https://doi.org/10.2174/187152711794480384
  9. Li-Weber M. New therapeutic aspects of flavones: the anticancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin // Cancer Treat Rev. - 2009. - N 35. - P. 57-68. DOI: https://doi.org/10.1016/j.ctrv.2008.09.005
  10. Kumagai T. Scutellaria baicalensis, a herbal medicine: anti-proliferative and apoptotic activity against acute lymphocytic leukemia, lymphoma and myeloma cell lines / T. Kumagai, C.I. Muller, J.C. Desmond, Y. Imai, D. Heber, H.P. Koeffler // Leuk Res. - 2007. - N 31. - P. 523-530. DOI: https://doi.org/10.1016/j.leukres.2006.08.019
  11. Himeji M. Difference of growth-inhibitory effect of Scutellaria baicalensis-producing flavonoid wogonin among human cancer cells and normal diploid cell / M. Himeji, T. Ohtsuki, H. Fukazawa, M. Tanaka, S. Yazaki, S. Ui, K. Nishio, H. Yamamoto, K. Tasaka, A. Mimura // Cancer Lett. - 2007. - N 245. - P. 269-274. DOI: https://doi.org/10.1016/j.canlet.2006.01.011
  12. Huang S.T. Wogonin, an active compound in Scutellaria baicalensis, induces apoptosis and reduces telomerase activity in the HL-60 leukemia cells / S.T. Huang, C.Y. Wang, R.C. Yang, C.J. Chu, H.T. Wu, J.H. Pang // Phytomedicine. - 2010. - N 17. - P. 47-54. DOI: https://doi.org/10.1016/j.phymed.2009.06.005
  13. Ozmen A. In vitro anti-leukemic activity of the ethno-pharmacological plant Scutellaria orientalis ssp. carica endemic to western Turkey / A. Ozmen// Phytomedicine. - 2010. - N 17. - P. 55-62. DOI: https://doi.org/10.1016/j.phymed.2009.06.001
  14. Ikezoe T. Baicalin is a major component of PC-SPES which inhibits the proliferation of human cancer cells via apoptosis and cell cycle arrest / T. Ikezoe, S.S. Chen, D. Heber, H. Taguchi, H.P. Koeffler // Prostate. 2001. - N 49. - P. 285-292. DOI: https://doi.org/10.1002/pros.10024
  15. Ciesielska E., Gwardys A., Metodiewa D. Anticancer, antiradical and antioxidative actions of novel Antoksyd S and its major components, baicalin and baicalein // Anticancer Res. - 2002. - Vol. 22(5). - P. 2885-2891.
  16. Ciesielska E. In vitro antileukemic, antioxidant and prooxidant activities of Antoksyd S (C/E/XXI): a comparison with baicalin and baicalein / E. Ciesielska, M. Wolszczak, B. Gulanowski, A. Szulawska, A. Kochman, D. Metodiewa // In Vivo. - 2004. - Vol. 18(4). - P. 497-503.
  17. Lin X.C. Inhibitory effect and its kinetic analysis of baicalein on recombinant human protein kinase CK2 holoenzyme / X.C. Lin, X.G. Liu, X.W. Chen, W.Z. Chen, N.C. Liang // Ai Zheng. - 2004. - Vol. 23(8). - P. 874-878.
  18. Chen Y.J. Baicalein Triggers Mitochondria-Mediated Apoptosis and Enhances the Antileukemic Effect of Vincristine in Childhood Acute Lymphoblastic Leukemia CCRF-CEM Cells / Y.J. Chen, C.S. Wu, J.J. Shieh, J.H. Wu, H.Y. Chen, T.W. Chung, Y.K. Chen, C.C. Lin // Evid Based Complement Alternat Med. - 2013. Vol. 2013. - P. 124747. DOI: http://dx.doi.org/10.1155/2013/124747
  19. Yu C. Different extraction pretreatments significantly change the flavonoid contents of Scutellaria baicalensis / C. Yu, F. Qu, Y. Mao, D. Li, Z. Zhen, R. Nass, T. Calway, Y. Wang, C.S. Yuan, C.Z. Wang // Pharm Biol. - 2013. - Vol. 51(10). - P. 1228-1235. DOI:10.3 109/13880209.2013.784922
  20. Li J.H., Wang L.S., Zou J.M. Study on degradation of baicalin by endogenous enzymes in water extraction of Scutellaria baicalensis // Chin Trad Herb Drugs. - 2009. - N 40. - P. 397-400.
  21. Sasaki K. Molecular characterization of a novel beta-glucuronidase from Scutellaria baicalensis Georgi. / K. Sasaki, F. Taura, Y. Shoyama, S. Morimoto // J Biol Chem. - 2000. - Vol. 275. - P. 27466-27472.
  22. Государственная фармакопея Российской Федерации. XIV издание. Том I. - Москва: Министерство здравоохранения Российской Федерации, 2018.
  23. Zhilyakova E.T. Studying the polyphenolic structure of Laurus Nobilis L. leaves / E.T. Zhilyakova, O.O. Novikov, D.I. Pisarev, A.Y. Malyutina, N.N. Boyko // Indo Am. J. Pharm. Sci. - 2017. - Vol. 4(9). - P. 3066-74. DOI: http://doi.org/10.5281/zenodo.910685

版权所有 © Boyko N.N., Pisarev D.I., Zhilyakova E.T., Maljutina A.Y., Novikov O.O., Bocharnikova M.A., 2019

Creative Commons License
此作品已接受知识共享署名 4.0国际许可协议的许可
 
##common.cookie##