Applicability assessment for seed endophytic fungal microbiome structure in the breeding of soft spring wheat
- Authors: Duksi F.1,2, Bondarenko G.N.1,2, Burlutskiy V.A.2,3, Tsvetkova Y.V.1, Dekin G.O.2, Davydova N.V.4
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Affiliations:
- All-Russian Plant Quarantine Center
- Peoples' Friendship University of Russia named after Patrice Lumumba
- Kaluga Research Agriculture Institute - branch of Russian Potato Research Centre
- Federal Research Center "Nemchinovka"
- Issue: Vol 27, No 4 (2025)
- Pages: 70-84
- Section: Agrochemistry, agrosoil science, plant protection and quarantine
- Submitted: 18.10.2025
- Published: 07.11.2025
- URL: https://journals.rcsi.science/1991-6639/article/view/333183
- DOI: https://doi.org/10.35330/1991-6639-2025-27-4-70-84
- EDN: https://elibrary.ru/DPOYQK
- ID: 333183
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Abstract
The paper presents the research outcomes for community structure of the endophytic fungi in the seed of some new highly productive genotypes in soft spring wheat, created using complex breeding and genetic methodologies: haplo-biotechnology, the ecological and geographical selecting parental forms in the design of complex spring-winter hybrids (ecological valents), to reveal the possibility of evaluating breeding material when breeding new commercial varieties.
Aim. To investigate the endophytic community (pathogenic and non-pathogenic), in wheat seeds to determine the feasibility of this type of evaluation to assess resistance to fungal diseases, as well as to understand the complex interactions between these fungi, plants and the environment.
Materials and methods. Genotypes of soft spring wheat created using modern breeding and genetic approaches (haplo-biotechnology, ecological and geographical selection of parental pairs in the construction of complex spring-winter hybrids). A microbiological technique is used (isolating the pathogen on a growth medium, obtaining a pure culture), which includes morphological identification (microscopy). The initial diagnosis is confirmed using polymerase chain reaction (PCR) based on primers developed for the (ITS) region, followed by Sanger sequencing.
Results. Differences are shown in the community structure of endophytic fungi in the seed material, depending on the breeding lines (genotypes) of spring wheat. 7 species of fungi belonging to 5 genera are identified. Pathogenic fungi from the genera Fusarium and Alternaria, as well as non-pathogenic fungi from Trichoderma and Clonostachys, are found among them.
Conclusions and recommendations. The results of this study contribute to a broader understanding of the formation of beneficial endophytic communities in agriculture and breeding (selection of resistant wheat varieties, which would reduce the need for pesticides). The study of such fungi is a promising area of research for the breeding of soft wheat.
About the authors
F. Duksi
All-Russian Plant Quarantine Center; Peoples' Friendship University of Russia named after Patrice Lumumba
Email: f.duksi@gmail.com
ORCID iD: 0000-0002-7353-7816
SPIN-code: 9937-6393
Agronomist Testing laboratory center; Postgraduate student
Russian Federation, Pogranichnaya street, 32, Bykovo urban settlement, Ramensky municipal district, Moscow region, Russia, 140150; Miklukho-Maklaya street, 6, Moscow, Russia, 117198G. N. Bondarenko
All-Russian Plant Quarantine Center; Peoples' Friendship University of Russia named after Patrice Lumumba
Email: f.duksi@gmail.com
ORCID iD: 0000-0002-2172-7634
SPIN-code: 2631-5209
Candidate of Biological Sciences, Senior Researcher, Head of the testing laboratory center; Associate Professor
Russian Federation, Pogranichnaya street, 32, Bykovo urban settlement, Ramensky municipal district, Moscow region, Russia, 140150; Miklukho-Maklaya street, 6, Moscow, Russia, 117198V. A. Burlutskiy
Peoples' Friendship University of Russia named after Patrice Lumumba; Kaluga Research Agriculture Institute - branch of Russian Potato Research Centre
Email: burlutskiy-va@rudn.ru
ORCID iD: 0000-0002-5072-4766
SPIN-code: 4167-4646
Candidate of Agricultural Sciences, Senior Lecturer
Russian Federation, Miklukho-Maklaya street, 6, Moscow, Russia, 117198; Tsentralnaya street, 2, Kaluga Experimental Agricultural Station, Peremyshlsky District, Kaluga Region, Russia, 249142Yu. V. Tsvetkova
All-Russian Plant Quarantine Center
Email: f.duksi@gmail.com
SPIN-code: 2744-1123
Researcher, Mycology Laboratory, Testing Laboratory Center
Russian Federation, Pogranichnaya street, 32, Bykovo urban settlement, Ramensky municipal district, Moscow region, Russia, 140150G. O. Dekin
Peoples' Friendship University of Russia named after Patrice Lumumba
Email: ghebrielokba@gmail.com
ORCID iD: 0009-0007-4460-1077
Researcher, Mycology Laboratory, Testing Laboratory Center
Russian Federation, Miklukho-Maklaya street, 6, Moscow, Russia, 117198N. V. Davydova
Federal Research Center "Nemchinovka"
Author for correspondence.
Email: f.duksi@gmail.com
SPIN-code: 5470-3052
Doctor of Agricultural Sciences, Head of the Laboratory of Selection and Primary Seed Production of Spring Wheat
Russian Federation, Agrokhimikov street, 6, Novoivanovskoye, Odintsovo, Moscow Region, Russia, 143026References
- Shalaeva L.V. World and Russian grain market: assessment of trends and prospects. Food policy and security. 2023. Vol. 10. No. 2. Pp. 287-302. doi: 10.18334/ppib.10.2.117014. (In Russian)
- Slovareva O.Yu. Production, export and import of cereals and compilation of a list of phytopathogenic bacteria associated with them. Agrarian Bulletin of the North Caucasus. 2023. No. 3(51). Pp. 47-54. doi: 10.31279/2949-4796-2023-3-51-47-54 (In Russian)
- Mal'chikov P.N., Myasnikova M.G. Development of breeding of spring durum wheat in Russia (former USSR countries), results and prospects. Vavilov Journal of Genetics and Breeding. 2023. Vol. 27. No. 6. Pp. 591-608. doi: 10.18699/VJGB-23-71. (In Russian)
- Gen A.A., Burlutskiy V.A., Gringof I.G. et al. Photometric and ultrasonic methods for assessing the condition of barley crops. Hydrometeorology and education. 2025. No. 1. Pp. 76-93. EDN: LBNGJM. (In Russian)
- Gagkaeva T., Gavrilova O., Orina A. et al. Analysis of toxigenic fusarium species associated with wheat grain from three regions of Russia: Volga, Ural, and West Siberia. Toxins. 2019. Vol. 11. No. 5. P. 252. doi: 10.3390/toxins11050252
- Pigalov A.V., Soloviev A.A., Garibyan Ts.S. Effects of fusarium fungi on the yield and quality of wheat and triticale and the possibility of using RNA interference and small RNA in the fight against fungal diseases. In: International Congress "VIII Congress of the Vavilov Society of Geneticists and Breeders, dedicated to the 300th anniversary of Russian science and higher education". Collection of abstracts. St. Petersburg, 2024. P. 663. (In Russian)
- Chumakov A.E., Minkevich I.I., Vlasov Yu.I., Gavrilova E.A. Basic methods of phytopathological research. Moscow: Kolos, VNIIZR, 1974. Pp. 131-139. (In Russian)
- EPPO Standard PM 7/86 (1). EPPO Bulletin. 2009. Vol. 39. Pp. 18-24. doi: 10.1111/j.1365-2338.2009.02245.x
- White T., Bruns T., Lee S., Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols, a Guide to Methods and Applications. San Diego: Academic Press. 1990. Pp. 315-322. doi: 10.1016/B978-0-12-372180-8.50042-1
- Kuznetsova A.A., Tsvetkova Yu.V., Kamchenkov A.V. Culture morphological characteristics of the pathogen Diaporthe vaccinii in regulated products - cranberry plants. Plant Health and Quarantine. 2021. No. 2. Pp. 27-36. doi: 10.69536/FKR.2021.49.45.001. (In Russian)
- Belkin D.L., Bondarenko G.N., Yaremko A.B., Uvarova D.A. Sequencing method by types for identifying quarantine pests. Karantin rastenij. Nauka i praktika [Plant Quarantine. Science and Practice]. 2019. No. 2(28). Pp. 31-34. (In Russian)
- Rauwane M.E., Ogugua U.V., Kalu C.M. et al. Pathogenicity and virulence factors of fusarium graminearum including factors discovered using next generation sequencing technologies and proteomics. Microorganisms. 2020. Vol. 8. No. 2. P. 305. doi: 10.3390/microorganisms8020305
- Bianchini A., Battilani P. Climate change and Fusarium mycotoxins: A review of trends and predictions. Food Additives & Contaminants: Part A. 2021. Vol. 38. No. 6. Pp. 943-954. doi: 10.1080/19440049.2021.1914864
- Gizachew H.R. Harnessing and sounding the alarm on fusarium head blight of wheat: current status, biology, detection and diagnosis method, mycotoxins, and integrated management options. Greener Journal of Agricultural Sciences. 2023. Vol. 13. No. 4. Pp. 227-243.
- Awuchi C.G., Ondari E.N., Ogbonna C.U. et al. Mycotoxins affecting animals, foods, humans, and plants: types, occurrence, toxicities, action mechanisms, prevention, and detoxification strategies - a revisit. Foods. 2021. Vol. 10. No. 6. P. 1279. doi: 10.3390/foods10061279
- Türkölmez Ş., Özer G., Derviş S. Clonostachys rosea Strain ST1140: An endophytic plant-growth-promoting fungus, and its potential use in seedbeds with wheat-grain substrate. Current Microbiology. 2022. Vol. 80. No. 1. P. 36. doi: 10.1007/s00284-022-03146-3
- Zin N.A., Badaluddin N.A. Biological functions of Trichoderma spp. for agriculture applications. Annals of Agricultural Sciences. 2020. Vol. 65. No. 2. Pp. 168-178. doi: 10.1016/j.aoas.2020.09.003
- Bernal S.P.F., Gritti M.A., Dos Santos V.P. et al. Pharmaceutical biotechnological potential of filamentous fungi isolated from textile industry. Archives of Microbiology. 2021. Vol. 203. No. 7. Pp. 3933-3944. doi: 10.1007/s00203-021-02379-3
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