Analysis of exo- and endocytosis in the mouse nerve ending in experimental diabetes mellitus
- 作者: Yakovleva O.1, Zakharov A.1,2, Zefirov A.2, Sitdikova G.1
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隶属关系:
- Institute of Fundamental Medicine and Biology
- Kazan Medical University
- 期: 卷 11, 编号 2 (2017)
- 页面: 177-186
- 栏目: Articles
- URL: https://journals.rcsi.science/1990-7478/article/view/213249
- DOI: https://doi.org/10.1134/S199074781702009X
- ID: 213249
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详细
Diabetes mellitus (DM) is a systemic disease characterized by changes in many organs and tissues, including the motor system. The processes of exo- and endocytosis in the motor nerve ending of the mouse diaphragm muscle during high-frequency activity in experimental alloxan model of DM were studied. Endplate potentials (EPPs) were recorded using intracellular microelectrodes during single and high-frequency (50 Hz, 1 min) stimulation. In mice with the experimental DM, the amplitude-time parameters of EPPs did not differ from those of the control; however, an increase in EPPs depression and a slower recovery were observed during high-frequency stimulation. Using an endocytosis marker FM 1-43, it was shown that in animals with experimental DM fluorescence intensity of the nerve terminals loaded with the dye by high-frequency stimulation increased that was prevented by 1-azakenpaullone (2 μM), an inhibitor of slow dynamin-1-mediated endocytosis. In addition, in the model animals, the destaining of the pre-loaded nerve terminals during high-frequency (50 Hz) stimulation slowed down. The obtained data indicate that in the experimental first type DM recycling of synaptic vesicles via long path becomes more pronounced and the mechanisms of the vesicular transport are impaired, which was confirmed by methods of mathematical modeling.
作者简介
O. Yakovleva
Institute of Fundamental Medicine and Biology
编辑信件的主要联系方式.
Email: a-olay@yandex.ru
俄罗斯联邦, ul. Kremlevskaya 18, Kazan, 420008
A. Zakharov
Institute of Fundamental Medicine and Biology; Kazan Medical University
Email: a-olay@yandex.ru
俄罗斯联邦, ul. Kremlevskaya 18, Kazan, 420008; ul. Butlerova 49, Kazan, 420039
A. Zefirov
Kazan Medical University
Email: a-olay@yandex.ru
俄罗斯联邦, ul. Butlerova 49, Kazan, 420039
G. Sitdikova
Institute of Fundamental Medicine and Biology
Email: a-olay@yandex.ru
俄罗斯联邦, ul. Kremlevskaya 18, Kazan, 420008