Effects of native and oxidized apolipoprotein A-I on lipid bilayer microviscosity of erythrocyte plasma membrane
- 作者: Mokrushnikov P.1,2, Dudarev A.1, Tkachenko T.1, Gorodetskaya A.1, Usynin I.1
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隶属关系:
- Research Institute of Biochemistry
- Novosibirsk State University of Architecture and Civil Engineering
- 期: 卷 11, 编号 1 (2017)
- 页面: 48-53
- 栏目: Articles
- URL: https://journals.rcsi.science/1990-7478/article/view/213230
- DOI: https://doi.org/10.1134/S1990747816040164
- ID: 213230
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详细
Using a pyrene as a fluorescent probe, we investigated the influence of native and oxidized apolipoprotein A-I (apo A-I) and their complexes with tetrahydrocortisol (THC) on the microviscosity of the erythrocyte plasma membrane. The addition of THC to isolated membranes led to a 17% increase in the membrane microviscosity. In contrast, native apo A-I reduced the microviscosity (i.e., increased the fluidity) of the membranes by 15%. A more pronounced increase (by 25%) in the membrane fluidity was found in the presence of the complex of apo A-I with THC. Unlike native apo A-I, oxidized apo A-I and its complex with THC did not change the membrane viscosity. In view of the fact that apo A-I plays an important role in the binding of membrane cholesterol we suggest that the observed increase in the membrane fluidity under the influence of the native apo A-I is associated with the cholesterol efflux from plasma membrane. Oxidative modification of apo A-I likely disturbs the mechanisms of the cholesterol efflux and prevents the decrease in the membrane microviscosity.
作者简介
P. Mokrushnikov
Research Institute of Biochemistry; Novosibirsk State University of Architecture and Civil Engineering
编辑信件的主要联系方式.
Email: pwm64@ngs.ru
俄罗斯联邦, ul. Timakova 2, Novosibirsk, 630117; ul. Leningradskaya 113, Novosibirsk, 630008
A. Dudarev
Research Institute of Biochemistry
Email: pwm64@ngs.ru
俄罗斯联邦, ul. Timakova 2, Novosibirsk, 630117
T. Tkachenko
Research Institute of Biochemistry
Email: pwm64@ngs.ru
俄罗斯联邦, ul. Timakova 2, Novosibirsk, 630117
A. Gorodetskaya
Research Institute of Biochemistry
Email: pwm64@ngs.ru
俄罗斯联邦, ul. Timakova 2, Novosibirsk, 630117
I. Usynin
Research Institute of Biochemistry
Email: pwm64@ngs.ru
俄罗斯联邦, ul. Timakova 2, Novosibirsk, 630117