The effect of dehydroepiandrosterone on inflammatory response of astroglial cells
- Авторы: Buyanova S.1,2, Chistyakov D.2, Astakhova A.2, Sergeeva M.2
-
Учреждения:
- Faculty of Bioengineering and Bioinformatics
- Belozersky Institute of Physico-Chemical Biology
- Выпуск: Том 11, № 4 (2017)
- Страницы: 304-310
- Раздел: Articles
- URL: https://journals.rcsi.science/1990-7478/article/view/213274
- DOI: https://doi.org/10.1134/S199074781704002X
- ID: 213274
Цитировать
Аннотация
An increased interest in neuroinflammation is conditioned by its involvement in various pathological processes in the brain. Astrocytes play an important role in neuroinflammation, participating in its regulation, throwing out a large number of signaling molecules. Steroid compounds, actively produced by astrocytes, are of interest with regards to the regulation of inflammatory processes in the central nervous system. In the present work the effect of dehydroepiandrosterone (DHEA) on astroglial cells (cultured primary rat astrocytes) in a model of inflammation was studied. The inflammatory response was stimulated with lipopolysaccharide (LPS). Expression levels of pro-inflammatory factor TNFα, antinflammatory interleukin IL-10, and both pro- and antiinflammatory protein COX-2 were measured. The expression of IL-10, COX-2, and TNFα mRNA was determined by real-time PCR, COX-2 protein level by immunoblotting method, TNFα and IL-10 release by enzyme immunoassay. The effect of short-term (30 min) and long-term (24 h) exposure to DHEA was evaluated. It was shown that DHEA potentiates LPS-stimulated (1) increase in the IL-10 mRNA level; (2) IL-10 release; (3) does not affect TNFα level, and (4) exerts a weak pulsating bidirectional effect on COX-2. Using trilostane, an inhibitor of 3β-hydroxysteroid dehydrogenase, a key enzyme of DHEA metabolism, it was shown that DHEA metabolites make the main contribution to its effect. Thus, DHEA is of interest as a stimulant of anti-inflammatory processes in the brain.
Об авторах
S. Buyanova
Faculty of Bioengineering and Bioinformatics; Belozersky Institute of Physico-Chemical Biology
Автор, ответственный за переписку.
Email: sofia.buyanova@gmail.com
Россия, Moscow, 119234; Moscow, 119992
D. Chistyakov
Belozersky Institute of Physico-Chemical Biology
Email: sofia.buyanova@gmail.com
Россия, Moscow, 119992
A. Astakhova
Belozersky Institute of Physico-Chemical Biology
Email: sofia.buyanova@gmail.com
Россия, Moscow, 119992
M. Sergeeva
Belozersky Institute of Physico-Chemical Biology
Email: sofia.buyanova@gmail.com
Россия, Moscow, 119992