Forskolin As a Neuroprotector and Modulator of Glutamate-Evoked Ca2+ Entry into Cerebellar Neurons
- Авторы: Ivanova M.A.1, Sitnikova L.S.1, Stepanenko Y.D.1, Karelina T.V.1, Sibarov D.A.1, Abushik P.A.1, Antonov S.M.1
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Учреждения:
- Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences
- Выпуск: Том 12, № 4 (2018)
- Страницы: 390-393
- Раздел: Short Communication
- URL: https://journals.rcsi.science/1990-7478/article/view/213331
- DOI: https://doi.org/10.1134/S1990747818050021
- ID: 213331
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Аннотация
The analysis of neuronal viability of cerebellar neurons in primary culture during long-lasting (240 min) treatment with glutamate revealed that forskolin (1 μM), an adenylate cyclase activator, prevents apoptosis and necrosis of cells, thus exhibiting neuroprotective properties. As the cytotoxic action of glutamate causes an increase of the intracellular Ca2+ concentration, we further studied forskolin influence on glutamate-evoked Ca2+ responses of neurons. A short-time pretreatment with 1 μM forskolin significantly decreases Ca2+ entry into neurons during glutamate action. The obtained results demonstrate an important role of adenylate cyclase and cAMP in regulation of the Ca2+ entry into neurons and intracellular signaling pathways preventing neuronal death of cerebellar neurons in excytotoxic stress.
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Об авторах
M. Ivanova
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences
Email: polinaabushik@gmail.com
Россия, St. Petersburg, 194223
L. Sitnikova
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences
Email: polinaabushik@gmail.com
Россия, St. Petersburg, 194223
Yu. Stepanenko
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences
Email: polinaabushik@gmail.com
Россия, St. Petersburg, 194223
T. Karelina
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences
Email: polinaabushik@gmail.com
Россия, St. Petersburg, 194223
D. Sibarov
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences
Email: polinaabushik@gmail.com
Россия, St. Petersburg, 194223
P. Abushik
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences
Автор, ответственный за переписку.
Email: polinaabushik@gmail.com
Россия, St. Petersburg, 194223
S. Antonov
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences
Email: polinaabushik@gmail.com
Россия, St. Petersburg, 194223
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