Enviar artigo por via de e-mail Membrane TNFα: Importance for the Effector Function of Dendritic Cells and Potential Ways of Its Targeted Modulation
- Autores: Tyrinova T.1, Mishinov S.2, Leplina O.1, Dolgova E.3, Proskurina A.3, Batorov E.1, Tikhonova M.1, Kurochkina Y.1, Oleynik E.1, Kalinovskiy A.4, Chernov S.4, Stupak V.2, Bogachev S.3, Ostanin A.1, Chernykh E.1
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Afiliações:
- Scientific Research Institute of Fundamental and Clinical Immunology
- Scientific Research Institute of Traumatology and Orthopedics named after Ya.L. Tsivian
- Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
- Federal Neurosurgical Center
- Edição: Volume 12, Nº 3 (2018)
- Páginas: 247-254
- Seção: Articles
- URL: https://journals.rcsi.science/1990-7478/article/view/213318
- DOI: https://doi.org/10.1134/S199074781803008X
- ID: 213318
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Resumo
Membrane TNFα (mTNFα) is expressed on many immune cell types and performs various biological functions. Dendritic cells (DC) of high-grade glioma patients exhibit impaired cytotoxic activity against TNFα-sensitive HEp-2 tumor cells. The mechanisms leading to the impairment of the TNFα- dependent tumoricidal activity of DC and the possibility of regulating the cytotoxic activity of DC mediated by the TNFα/TNF-R1 signaling pathway have been studied. The study was conducted on healthy donors and patients with newly diagnosed high-grade glioma. DC were generated by culturing the plastic-adherent peripheral blood mononuclear cell fraction in the presence of GM-CSF and interferon-α (IFN-DC). It was shown that the impairment of the cytotoxic activity of patient IFN-DC was associated with a low number of DC expressing mTNFα and a low level of TNFα mRNA expression in DC. IFN-DC of patients exhibited a tendency of high activity of the TNFα-converting enzyme (TACE), which accomplishes shedding of mTNFα from the cell membrane. An increased number of IFN-DC with mTNFα caused by TACE blocking enhanced cytotoxic activity of the patient’s IFN-DC against HEp-2 cells. It was established that exogenous interleukin-2 and extracellular DNA are up-regulators of the mTNFα expression on IFN-DC of the patients, but their effects are mediated by different mechanisms.
Sobre autores
T. Tyrinova
Scientific Research Institute of Fundamental and Clinical Immunology
Autor responsável pela correspondência
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630099
S. Mishinov
Scientific Research Institute of Traumatology and Orthopedics named after Ya.L. Tsivian
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630091
O. Leplina
Scientific Research Institute of Fundamental and Clinical Immunology
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630099
E. Dolgova
Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630090
A. Proskurina
Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630090
E. Batorov
Scientific Research Institute of Fundamental and Clinical Immunology
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630099
M. Tikhonova
Scientific Research Institute of Fundamental and Clinical Immunology
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630099
Yu. Kurochkina
Scientific Research Institute of Fundamental and Clinical Immunology
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630099
E. Oleynik
Scientific Research Institute of Fundamental and Clinical Immunology
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630099
A. Kalinovskiy
Federal Neurosurgical Center
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630087
S. Chernov
Federal Neurosurgical Center
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630087
V. Stupak
Scientific Research Institute of Traumatology and Orthopedics named after Ya.L. Tsivian
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630091
S. Bogachev
Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630090
A. Ostanin
Scientific Research Institute of Fundamental and Clinical Immunology
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630099
E. Chernykh
Scientific Research Institute of Fundamental and Clinical Immunology
Email: tyrinova@bk.ru
Rússia, Novosibirsk, 630099
