Enviar artigo por via de e-mail Detection of DNA molecules in a lipid nanotube channel in the low ion strength conditions
- Autores: Chekashkina K.1,2, Galimzyanov T.2,3, Kuzmin P.2, Akimov S.2,3, Romanov S.1,2,4, Pozmogova G.1, Klinov D.1, Bashkirov P.1,2
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Afiliações:
- Federal Research and Clinical Center of Physical-Chemical Medicine
- Frumkin Institute of Physical Chemistry and Electrochemistry
- National University of Science and Technology MISiS
- Moscow Institute of Physics and Technology
- Edição: Volume 11, Nº 3 (2017)
- Páginas: 217-224
- Seção: Articles
- URL: https://journals.rcsi.science/1990-7478/article/view/213254
- DOI: https://doi.org/10.1134/S1990747817030047
- ID: 213254
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Resumo
Investigation of the transport phenomena in the nanoscopic channels/pores with the diameter smaller than 100 nm is of utmost importance for various biological, medical, and technical applications. Presently, the main line of development of nanofluidics is creation of biosensors capable of detecting single molecules and manipulating them. Detection of molecules is based on the measurement of electric current through a channel of appropriate size: when the molecule enters the channel, which diameter is comparable with the molecule size, the ion current reduces. In order to improve transport properties of such channels, their walls are often coated with a lipid bilayer, which behaves as two-dimensional liquid and thus is capable of supporting transport phenomena. In the present work, we utilized this property of lipid membranes for the development of a method for detecting and controlling transport of single-stranded DNA through channels formed by membrane cylinders with the luminal radii of 5–7 nm. We have demonstrated that in the conditions of small ion strength, the appearance of a DNA molecule inside such channel is accompanied by an increase of its ion conductivity and can be controlled by the polarity of the applied voltage. The amplitude of the ion current increase allows evaluating the amount of DNA molecules inside the channels. It was also demonstrated that upon adsorption of DNA molecules on the lipid bilayer surface, the membrane cylinder behaves as a voltage-sensitive selective ion channel.
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Sobre autores
K. Chekashkina
Federal Research and Clinical Center of Physical-Chemical Medicine; Frumkin Institute of Physical Chemistry and Electrochemistry
Email: pavel.bashkirov@niifhm.ru
Rússia, ul. Malaya Pirogovskaya 1a, Moscow, 119435; Leninskiy pr. 31/5, Moscow, 119071
T. Galimzyanov
Frumkin Institute of Physical Chemistry and Electrochemistry; National University of Science and Technology MISiS
Email: pavel.bashkirov@niifhm.ru
Rússia, Leninskiy pr. 31/5, Moscow, 119071; Leninskiy pr. 4, Moscow, 119049
P. Kuzmin
Frumkin Institute of Physical Chemistry and Electrochemistry
Email: pavel.bashkirov@niifhm.ru
Rússia, Leninskiy pr. 31/5, Moscow, 119071
S. Akimov
Frumkin Institute of Physical Chemistry and Electrochemistry; National University of Science and Technology MISiS
Email: pavel.bashkirov@niifhm.ru
Rússia, Leninskiy pr. 31/5, Moscow, 119071; Leninskiy pr. 4, Moscow, 119049
S. Romanov
Federal Research and Clinical Center of Physical-Chemical Medicine; Frumkin Institute of Physical Chemistry and Electrochemistry; Moscow Institute of Physics and Technology
Email: pavel.bashkirov@niifhm.ru
Rússia, ul. Malaya Pirogovskaya 1a, Moscow, 119435; Leninskiy pr. 31/5, Moscow, 119071; Institutskiy per. 9, Dolgoprudniy, 141700
G. Pozmogova
Federal Research and Clinical Center of Physical-Chemical Medicine
Email: pavel.bashkirov@niifhm.ru
Rússia, ul. Malaya Pirogovskaya 1a, Moscow, 119435
D. Klinov
Federal Research and Clinical Center of Physical-Chemical Medicine
Email: pavel.bashkirov@niifhm.ru
Rússia, ul. Malaya Pirogovskaya 1a, Moscow, 119435
P. Bashkirov
Federal Research and Clinical Center of Physical-Chemical Medicine; Frumkin Institute of Physical Chemistry and Electrochemistry
Autor responsável pela correspondência
Email: pavel.bashkirov@niifhm.ru
Rússia, ul. Malaya Pirogovskaya 1a, Moscow, 119435; Leninskiy pr. 31/5, Moscow, 119071
