Human IL-36RA production in Escherichia coli with coexpression of E. coli methionine aminopeptidase. II. Comparison of IL-36RA biological activity from different strains
- 作者: Kolobov A.1,2, Kondratyeva E.1, Sharafutdinova T.1, Kalinin R.1,2, Nimiritsky P.2, Stefanov V.2, Petrov A.1
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隶属关系:
- Institute of Highly Pure Biopreparations
- Department of Biochemistry
- 期: 卷 11, 编号 6 (2017)
- 页面: 453-457
- 栏目: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212501
- DOI: https://doi.org/10.1134/S1990519X17060074
- ID: 212501
如何引用文章
详细
Proinflammatory cytokines of the interleukin-36 (IL-36) family are involved in the pathogenesis of different skin diseases in human and mice. Administration of exogenous IL-36 receptor antagonist (IL-36RA) may be an approach to therapy of different dermatitises. For its full biological activity, IL-36RA requires cleavage of N-terminal methionine residue. We created three E. coli strains producing IL-36RA coexpressed with E. coli methionine aminopeptidase under control of different promoters. To test the biological activity of IL-36RA from different strains we transfected А549 cells with plasmid carrying the IL-36 receptor gene (IL1RL2). These cells respond to IL-36g treatment with production of IL-8, which can be quantified with ELISA. IL-36RA treatment disrupts IL-36 receptor activation by IL-36g and production of IL-8. Using this system, we proved that IL-36RA from all three producer strains is fully biologically active.
作者简介
A. Kolobov
Institute of Highly Pure Biopreparations; Department of Biochemistry
编辑信件的主要联系方式.
Email: alexey.kolobov.spb@gmail.com
俄罗斯联邦, St. Petersburg, 197110; St. Petersburg, 199004
E. Kondratyeva
Institute of Highly Pure Biopreparations
Email: alexey.kolobov.spb@gmail.com
俄罗斯联邦, St. Petersburg, 197110
T. Sharafutdinova
Institute of Highly Pure Biopreparations
Email: alexey.kolobov.spb@gmail.com
俄罗斯联邦, St. Petersburg, 197110
R. Kalinin
Institute of Highly Pure Biopreparations; Department of Biochemistry
Email: alexey.kolobov.spb@gmail.com
俄罗斯联邦, St. Petersburg, 197110; St. Petersburg, 199004
P. Nimiritsky
Department of Biochemistry
Email: alexey.kolobov.spb@gmail.com
俄罗斯联邦, Moscow, 119192
V. Stefanov
Department of Biochemistry
Email: alexey.kolobov.spb@gmail.com
俄罗斯联邦, St. Petersburg, 199004
A. Petrov
Institute of Highly Pure Biopreparations
Email: alexey.kolobov.spb@gmail.com
俄罗斯联邦, St. Petersburg, 197110