The MEK/ERK pathway is essential for maintenance of cytoprotective autophagy in E1A+cHA-RAS transformants after exposure to radiation
- Авторы: Kochetkova E.1, Blinova G.1, Zubova S.1, Bykova T.1, Pospelov V.1, Pospelova T.1
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Учреждения:
- Institute of Cytology
- Выпуск: Том 11, № 4 (2017)
- Страницы: 268-274
- Раздел: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212397
- DOI: https://doi.org/10.1134/S1990519X1704006X
- ID: 212397
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Аннотация
Autophagy is a conserved process of protein and organelle degradation that serves to maintain cell viability. Autophagy is frequently induced in response to stress or to exposure to DNA-damaging agents or retinoids, as well as to starvation and deficiency of growth factors. In this work, autophagy induced in E1A+cHA-RAS transformed cells in response to X-ray radiation was studied, with a focus on the role of the MEK/ERK signaling pathway in the regulation of radiation-induced autophagy. It was found that inhibition of the MEK/ERK pathway diminished cell viability and altered the sequence of events in radiation-induced autophagy. In particular, it caused aberrations in its final stages, leading to cytoplasmic accumulation of the p62/SQSTM1 adaptor protein in autophagic cavities of unclear origin. Thus, the MEK/ERK pathway activity is essential for the induction and maintenance of autophagy, increasing the viability of exposed cells in response to radiation.
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Об авторах
E. Kochetkova
Institute of Cytology
Автор, ответственный за переписку.
Email: lena.linnaea@gmail.com
Россия, St. Petersburg, 194064
G. Blinova
Institute of Cytology
Email: lena.linnaea@gmail.com
Россия, St. Petersburg, 194064
S. Zubova
Institute of Cytology
Email: lena.linnaea@gmail.com
Россия, St. Petersburg, 194064
T. Bykova
Institute of Cytology
Email: lena.linnaea@gmail.com
Россия, St. Petersburg, 194064
V. Pospelov
Institute of Cytology
Email: lena.linnaea@gmail.com
Россия, St. Petersburg, 194064
T. Pospelova
Institute of Cytology
Email: lena.linnaea@gmail.com
Россия, St. Petersburg, 194064