Homologues of p48 Protein from Morula Cells of Ascidian Styela rustica in Other Species of Stolidobranchia
- Autores: Tylets M.1, Daugavet M.2, Savelieva A.3, Podgornaya O.1,2,4, Shaposhnikova T.1
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Afiliações:
- Department of Cytology and Histology, St. Petersburg State University
- Institute of Cytology, Russian Academy of Sciences
- National Scientific Center of Marine Biology, Zhirmunsky Institute of Marine Biology, FEB RAS
- Far Eastern Federal University
- Edição: Volume 13, Nº 5 (2019)
- Páginas: 388-396
- Seção: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/213013
- DOI: https://doi.org/10.1134/S1990519X19050080
- ID: 213013
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Resumo
Tunicata is an interesting phylogenetic group, at the base of the branch leading to Chordata. Ascidians (Tunicata, Ascidiacea) morula blood cells are involved in defense reactions and in the formation of the tunic extracellular matrix. The ascidian tunic is hardened as a result of sclerotization of matrix proteins due to the action of phenol oxidase enzyme, contained in morula cells. Morula cells of the ascidian Styela rustica contain two major proteins, the function of which is still unknown; one of them is a protein with molecular mass of 48 kDa – p48. The aim of present study was to search for possible homologues of p48 in ascidians belonging to the Stolidobranchia order: Styela coriacea (Styelidae), Molgula citrina (Molgulidae), Boltenia echinata, Halocynthia aurantium (Pyuridae) and to determine the tissue distribution of those homologues. In order to show the presence of p48 in the tissues of sea squirts we used indirect immunolabeling method on paraffin sections.It was shown that the antibodies bind with morula cells of Styela rustica, Styela coreacea, Boltenia echinata, with the tunic matrix in all studied species and with test cells of Styela rustica, Styela coreacea, Boltenia echinata, Molgula citrina. It gives us a ground to assume the existence of p48 homologues in all studied ascidians and to expect a common mechanism of their participation in the tunic formation, as possible substrates of the phenol oxidase system.
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Sobre autores
M. Tylets
Department of Cytology and Histology, St. Petersburg State University
Autor responsável pela correspondência
Email: Masana97@yandex.ru
Rússia, St. Petersburg, 199034
M. Daugavet
Institute of Cytology, Russian Academy of Sciences
Email: Masana97@yandex.ru
Rússia, St. Petersburg, 194064
A. Savelieva
National Scientific Center of Marine Biology, Zhirmunsky Institute of Marine Biology, FEB RAS
Email: Masana97@yandex.ru
Rússia, Vladivostok, 690041
O. Podgornaya
Department of Cytology and Histology, St. Petersburg State University; Institute of Cytology, Russian Academy of Sciences; Far Eastern Federal University
Email: Masana97@yandex.ru
Rússia, St. Petersburg, 199034; St. Petersburg, 194064; Vladivostok, 690922
T. Shaposhnikova
Department of Cytology and Histology, St. Petersburg State University
Email: Masana97@yandex.ru
Rússia, St. Petersburg, 199034