The effect of yeast Saccharomyces cerevisiae red pigment on the expression of cloned human α-synuclein
- Autores: Nevzglyadova O.1, Artemov A.1, Mikhailova E.1, Lyublinskaya O.1, Ozerova Y.1, Ivanova P.1, Kostyleva E.1, Soidla T.1
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Afiliações:
- Institute of Cytology
- Edição: Volume 10, Nº 4 (2016)
- Páginas: 264-276
- Seção: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212115
- DOI: https://doi.org/10.1134/S1990519X16040106
- ID: 212115
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Resumo
Two transgenic yeast strains expressing human α-synuclein were used to study the impact of yeast red pigment exhibiting antiamyloid properties. It has been demonstrated that the endogenous red pigment produced under special conditions in strains carrying an ade1 mutation inhibits the expression of the hybrid protein α-synuclein-GFP. This was evident from the reduced mean value of GFP fluorescence and diminished number of cells accumulating cytoplasmic inclusions of α-synuclein-GFP. Exogenous forms of the purified red pigment (natural, synthetic and hydrolyzed derivatives) differ from the endogenous red pigment by their effect on α-synuclein. Exogenous red pigments increased the number of both cells expressing GFP fluorescence and those containing cytoplasmic inclusions. However, both endogenous and exogenous red pigments reduced the cloned α-synuclein toxicity and resulted in redistribution of the α-synuclein in cells. α-Synuclein content decreased in cell lysate pellets and increased in supernatants.
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Sobre autores
O. Nevzglyadova
Institute of Cytology
Email: soidla39@gmail.com
Rússia, St. Petersburg, 194064
A. Artemov
Institute of Cytology
Email: soidla39@gmail.com
Rússia, St. Petersburg, 194064
E. Mikhailova
Institute of Cytology
Email: soidla39@gmail.com
Rússia, St. Petersburg, 194064
O. Lyublinskaya
Institute of Cytology
Email: soidla39@gmail.com
Rússia, St. Petersburg, 194064
Yu. Ozerova
Institute of Cytology
Email: soidla39@gmail.com
Rússia, St. Petersburg, 194064
P. Ivanova
Institute of Cytology
Email: soidla39@gmail.com
Rússia, St. Petersburg, 194064
E. Kostyleva
Institute of Cytology
Email: soidla39@gmail.com
Rússia, St. Petersburg, 194064
T. Soidla
Institute of Cytology
Autor responsável pela correspondência
Email: soidla39@gmail.com
Rússia, St. Petersburg, 194064