Ion Homeostasis during the Growth of Human Mesenchymal Stem Culture. I. Density-Dependent Changes of Intracellular K+ and Na+ Content and K+ Influxes
- Авторлар: Shatrova A.1, Vinogradova T.1, Domnina A.1, Zemelko V.1, Pugovkina N.1, Nikolsky N.1, Marakhova I.1
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Мекемелер:
- Institute of Cytology, Russian Academy of Sciences
- Шығарылым: Том 13, № 2 (2019)
- Беттер: 93-99
- Бөлім: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212881
- DOI: https://doi.org/10.1134/S1990519X19020111
- ID: 212881
Дәйексөз келтіру
Аннотация
In this study, we reported proliferation-related changes in cell K+ and Na+ content and K+ influxes in cultivated human mesenchymal stem cells (MSCs). The intracellular K+ content calculated per cell protein amount was found to decrease in the growing culture, whereas intracellular Na+ content was not significantly changed. It was also revealed that, at higher densities of an hMSC monolayer, the ouabain-sensitive K+ influx was decreased thus indicating a decline in Na+, K+ pump-mediated transport. We analyzed the cell cycle profiles of hMSC cultures and found that, under optimal culture conditions in high-density cultures, the decline in K+ content per cell protein was correlated with accumulation of G1 cells and slowing down cell proliferation in the population. It is concluded that cell K+ content per cell protein is an informative test for assessing the functional status of stem cells in vitro.
Негізгі сөздер
Авторлар туралы
A. Shatrova
Institute of Cytology, Russian Academy of Sciences
Email: iim@incras.ru
Ресей, St. Petersburg, 194064
T. Vinogradova
Institute of Cytology, Russian Academy of Sciences
Email: iim@incras.ru
Ресей, St. Petersburg, 194064
A. Domnina
Institute of Cytology, Russian Academy of Sciences
Email: iim@incras.ru
Ресей, St. Petersburg, 194064
V. Zemelko
Institute of Cytology, Russian Academy of Sciences
Email: iim@incras.ru
Ресей, St. Petersburg, 194064
N. Pugovkina
Institute of Cytology, Russian Academy of Sciences
Email: iim@incras.ru
Ресей, St. Petersburg, 194064
N. Nikolsky
Institute of Cytology, Russian Academy of Sciences
Email: iim@incras.ru
Ресей, St. Petersburg, 194064
I. Marakhova
Institute of Cytology, Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: iim@incras.ru
Ресей, St. Petersburg, 194064