Toxicity of miR-204-5p Inhibition for Melanoma B16 Cells in vitro and Mice in vivo
- Authors: Palkina N.V.1, Komina A.V.1, Aksenenko M.B.1, Belonogov R.N.1, Lavrentev S.N.1, Ruksha T.G.1
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Affiliations:
- Department of Pathophysiology
- Issue: Vol 12, No 4 (2018)
- Pages: 307-314
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212711
- DOI: https://doi.org/10.1134/S1990519X18040077
- ID: 212711
Cite item
Abstract
MiR-204-5p is an oncosuppressive microRNA the level of which diminishes in various cancer types. The aim of this study was to evaluate miR-204-5p inhibition of melanoma-cell viability, as well as to determine whether miR-204-5p transfection of melanoma B16 cells with miR-204-5p mimic/inhibitor affected the microRNA expression. Proliferation of transfected cells was slightly, probably nonspecifically, reduced after 96 h. LNA™ inhibitors produced toxic effects in vivo. miR-204-5p-mimic/inhibitor application resulted to a slight, most likely nonspecific, modulation of melanoma-cell proliferation after 96 h. Bioinformatic analysis revealed that miR-204-5p is involved in the estrogen signaling pathway, lysine degradation, signaling pathways regulating pluripotency of stem cells, melanogenesis, transcriptional deregulation in cancer. Injection of miR-204-5p LNA™ inhibitor into mice reduced the level pf miR-204-5p but was not toxic.
Keywords
About the authors
N. V. Palkina
Department of Pathophysiology
Email: tatyana_ruksha@mail.ru
Russian Federation, Krasnoyarsk, 660022
A. V. Komina
Department of Pathophysiology
Email: tatyana_ruksha@mail.ru
Russian Federation, Krasnoyarsk, 660022
M. B. Aksenenko
Department of Pathophysiology
Email: tatyana_ruksha@mail.ru
Russian Federation, Krasnoyarsk, 660022
R. N. Belonogov
Department of Pathophysiology
Email: tatyana_ruksha@mail.ru
Russian Federation, Krasnoyarsk, 660022
S. N. Lavrentev
Department of Pathophysiology
Email: tatyana_ruksha@mail.ru
Russian Federation, Krasnoyarsk, 660022
T. G. Ruksha
Department of Pathophysiology
Author for correspondence.
Email: tatyana_ruksha@mail.ru
Russian Federation, Krasnoyarsk, 660022