人脐带组织无细胞基质和水凝胶冻干物的抑菌作用
- 作者: Kondratenko A.A.1,2, Chernov V.E.1, Tovpeko D.V.1, Volov D.A.1, Beliy N.V.1, Zemlyanoy D.A.2, Kalyuzhnaya L.I.1
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隶属关系:
- Kirov Military Medical Academy
- State Pediatric Medical University
- 期: 卷 26, 编号 3 (2024)
- 页面: 361-372
- 栏目: Research paper
- URL: https://journals.rcsi.science/1682-7392/article/view/264257
- DOI: https://doi.org/10.17816/brmma629139
- ID: 264257
如何引用文章
详细
对来自人类脐带的基质和水凝胶的抑菌作用进行了研究。众所周知,以胚胎外器官(包括人类脐带)的细胞外基质为基础的生物仿生学很有希望满足再生医学和组织工程学的需求。 来自不同人体器官和组织的细胞外基质的无细胞产物对有意的细菌感染具有抵抗力。研究人员采用不同的方法对人脐带进行脱细胞处理,制成了两种无细胞基质,并对基于这两种基质的两种水凝胶的抑菌特性进行了评估。介绍了脐带水凝胶冻干物的两种临床应用。使用生化分析技术研究了人脐带无细胞基质和水凝胶的成分。利用培养技术评估了 Staphylococcus aureus 和 Escherichia coli 对脐带基质和水凝胶的敏感性,同时还研究了细菌的代谢活 动。需要注意的是,来自人类脐带的无细胞基质和水凝胶由胶原蛋白组成,并含有蛋白质和糖胺聚糖。在培养的最初 16 小时内,无论使用哪种洗涤剂制备水凝胶,都能发现水凝胶对 Escherichia coli 有可靠的抑菌作用。基质没有抑菌作用,这表明是结构成分的水解促进了具有抑菌活性的物质的释放。这种影响可能是由于对微生物代谢活动水平的影响。使用人脐带水凝胶冻干粉作为自体皮移植的添加剂,治疗两名志愿者的深部感染伤口,促进了伤口愈合,且未发生感染。一般来说,使用无细胞人类脐带水凝胶冻干物作为额外治疗,可以确保皮肤自体移植的接合,并为容易感染的大面积深层伤口的愈合创造条件。
作者简介
Albina A. Kondratenko
Kirov Military Medical Academy; State Pediatric Medical University
编辑信件的主要联系方式.
Email: vmeda-nio@mil.ru
ORCID iD: 0000-0002-8511-5864
SPIN 代码: 1668-3497
Cand. Sci. (Biol.)
俄罗斯联邦, Saint Petersburg; Saint PetersburgVladimir E. Chernov
Kirov Military Medical Academy
Email: vmeda-nio@mil.ru
ORCID iD: 0000-0002-2440-3782
SPIN 代码: 8315-1161
Cand. Sci. (Biol.)
俄罗斯联邦, Saint PetersburgDmitry V. Tovpeko
Kirov Military Medical Academy
Email: vmeda-nio@mil.ru
ORCID iD: 0000-0003-0286-3056
SPIN 代码: 3698-4656
junior researcher
俄罗斯联邦, Saint PetersburgDaniil A. Volov
Kirov Military Medical Academy
Email: vmeda-nio@mil.ru
ORCID iD: 0000-0003-1493-7622
SPIN 代码: 1797-6654
traumatologist
俄罗斯联邦, Saint PetersburgNikolay V. Beliy
Kirov Military Medical Academy
Email: vmeda-nio@mil.ru
ORCID iD: 0000-0002-9370-8678
SPIN 代码: 8676-3186
junior researcher
俄罗斯联邦, Saint PetersburgDmitry A. Zemlyanoy
State Pediatric Medical University
Email: vmeda-nio@mil.ru
ORCID iD: 0000-0003-4716-809X
SPIN 代码: 3871-7531
MD, Cand. Sci. (Med.)
俄罗斯联邦, Saint PetersburgLidiya I. Kalyuzhnaya
Kirov Military Medical Academy
Email: vmeda-nio@mil.ru
ORCID iD: 0000-0001-6698-4872
SPIN 代码: 1348-3306
MD, Dr. Sci. (Med.), assistant professor
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