Synthesis and study on activity in vitro of the high purity human butyrylcholinesterase conjugated with gold nanoparticles


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The aim of this research was to design a method of immobilization of high-purity human butyrylcholinesterase on the surface of gold nanoparticles preserving the activity of the enzyme. In order to achieve this aim, the method of fractionation and purification of human butyrylcholinesterase from plasma was modified. The synthesis of 15-nm gold nanoparticles was carried out by citrated method. A method of conjugation of the high-purity butyrylcholinesterase with gold nanoparticles was developed. It was found that the Immobilization of butyrylcholinesterase on the surface of gold nanoparticles resulted in a significant (to 23%) increase in the specific activity of the enzyme.

作者简介

O. Sokolov

Institute of Biochemistry and Physiology of Plants and Microorganisms

编辑信件的主要联系方式.
Email: sokolov@ibppm.sgu.ru
俄罗斯联邦, pr. Entuziastov 13, Saratov, 410015

N. Selivanov

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: sokolov@ibppm.sgu.ru
俄罗斯联邦, pr. Entuziastov 13, Saratov, 410015

V. Bogatyrev

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: sokolov@ibppm.sgu.ru
俄罗斯联邦, pr. Entuziastov 13, Saratov, 410015

O. Selivanova

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: sokolov@ibppm.sgu.ru
俄罗斯联邦, pr. Entuziastov 13, Saratov, 410015

Y. Velikorodnaya

Research Institute of Hygiene, Toxicology, and Occupational Pathology Federal Biomedical Agency

Email: sokolov@ibppm.sgu.ru
俄罗斯联邦, ul. Zemlyachki 12, Volgograd, 400048

A. Pocheptsov

Research Institute of Hygiene, Toxicology, and Occupational Pathology Federal Biomedical Agency

Email: sokolov@ibppm.sgu.ru
俄罗斯联邦, ul. Zemlyachki 12, Volgograd, 400048

B. Filatov

Research Institute of Hygiene, Toxicology, and Occupational Pathology Federal Biomedical Agency

Email: sokolov@ibppm.sgu.ru
俄罗斯联邦, ul. Zemlyachki 12, Volgograd, 400048

S. Shchyogolev

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: sokolov@ibppm.sgu.ru
俄罗斯联邦, pr. Entuziastov 13, Saratov, 410015

L. Dykman

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: sokolov@ibppm.sgu.ru
俄罗斯联邦, pr. Entuziastov 13, Saratov, 410015

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