Cloning and Functional Analysis of SaCLCc1, a Gene Belonging to the Chloride Channel Family (CLC), from the Halophyte Suaeda altissima (L.) Pall.
- Авторы: Nedelyaeva O.I.1, Shuvalov A.V.1, Mayorova O.V.1, Yurchenko A.A.2, Popova L.G.1, Balnokin Y.V.1,3, Karpichev I.V.1
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Учреждения:
- Timiryazev Institute of Plant Physiology
- Dobrzhansky Center for Genomic Bioinformatics
- Moscow State University
- Выпуск: Том 481, № 1 (2018)
- Страницы: 186-189
- Раздел: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/212342
- DOI: https://doi.org/10.1134/S1607672918040026
- ID: 212342
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Аннотация
One of the genes of the CLC (Chloride Channel) family, SaCLCc1, from the halophyte Suaeda altissima (L.) Pall. was cloned. To investigate the function of SaCLCc1, it was expressed in the S. cerevisiae deletion mutant Δgef1::LEU2 for the only gene of the CLC family in this organism. The growth of the transformed SaCLCc1-expressing mutant Δgef1 was restored when cells were grown in Fe2+-deficient YPEG medium, in minimal synthetic media SD and SR (pH 7.0), and in rich YPD medium containing Mn2+. The complementation of the Δgef1 mutant phenotype with the SaClCc1 gene indicates the involvement of the SaClCc1 protein in the transport of Cl– ions.
Об авторах
O. Nedelyaeva
Timiryazev Institute of Plant Physiology
Автор, ответственный за переписку.
Email: olga.nedelyaeva@yandex.ru
Россия, Moscow, 127276
A. Shuvalov
Timiryazev Institute of Plant Physiology
Email: olga.nedelyaeva@yandex.ru
Россия, Moscow, 127276
O. Mayorova
Timiryazev Institute of Plant Physiology
Email: olga.nedelyaeva@yandex.ru
Россия, Moscow, 127276
A. Yurchenko
Dobrzhansky Center for Genomic Bioinformatics
Email: olga.nedelyaeva@yandex.ru
Россия, St. Petersburg, 199034
L. Popova
Timiryazev Institute of Plant Physiology
Email: olga.nedelyaeva@yandex.ru
Россия, Moscow, 127276
Yu. Balnokin
Timiryazev Institute of Plant Physiology; Moscow State University
Email: olga.nedelyaeva@yandex.ru
Россия, Moscow, 127276; Moscow, 119234
I. Karpichev
Timiryazev Institute of Plant Physiology
Email: olga.nedelyaeva@yandex.ru
Россия, Moscow, 127276
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