Identification and Expression Analysis of Chitinase Genes in Pitchers of Nepenthes sp. during Development
- Авторлар: Filyushin M.A.1, Kochieva E.Z.1,2, Shchennikova A.V.1, Beletsky A.V.1, Mardanov A.V.1, Ravin N.V.1, Skryabin K.G.1,2
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Мекемелер:
- Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences
- Faculty of Biotechnology, Moscow State University
- Шығарылым: Том 484, № 1 (2019)
- Беттер: 29-32
- Бөлім: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/212701
- DOI: https://doi.org/10.1134/S1607672919010083
- ID: 212701
Дәйексөз келтіру
Аннотация
Fifteen chitinases of classes I–V were identified in the transcriptomes of pitchers and adult leaves of the carnivorous plant Nepenthes sp. Ten of these chitinases were identified for the first time, including the chitinases of classes II and V. The expression levels of all found chitinase genes in leaves and at three stages of pitcher development were determined. The maximum level of transcriptional activity in an open pitcher was observed for the genes encoding chitinase NChi4 (class II) and its isoforms. The expression levels of these genes significantly increased as the pitcher developed. In addition, for the first time, transcription of the genes encoding chitinases of all five classes was detected in the leaves of this plant.
Авторлар туралы
M. Filyushin
Institute of Bioengineering, Research Centerof Biotechnology, Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: michel7753@mail.ru
Ресей, Moscow, 119071
E. Kochieva
Institute of Bioengineering, Research Centerof Biotechnology, Russian Academy of Sciences; Faculty of Biotechnology, Moscow State University
Email: michel7753@mail.ru
Ресей, Moscow, 119071; Moscow, 119234
A. Shchennikova
Institute of Bioengineering, Research Centerof Biotechnology, Russian Academy of Sciences
Email: michel7753@mail.ru
Ресей, Moscow, 119071
A. Beletsky
Institute of Bioengineering, Research Centerof Biotechnology, Russian Academy of Sciences
Email: michel7753@mail.ru
Ресей, Moscow, 119071
A. Mardanov
Institute of Bioengineering, Research Centerof Biotechnology, Russian Academy of Sciences
Email: michel7753@mail.ru
Ресей, Moscow, 119071
N. Ravin
Institute of Bioengineering, Research Centerof Biotechnology, Russian Academy of Sciences
Email: michel7753@mail.ru
Ресей, Moscow, 119071
K. Skryabin
Institute of Bioengineering, Research Centerof Biotechnology, Russian Academy of Sciences; Faculty of Biotechnology, Moscow State University
Email: michel7753@mail.ru
Ресей, Moscow, 119071; Moscow, 119234
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