Email this article Modification of human serum albumin under induced oxidation
- Authors: Gorobets M.G.1, Wasserman L.A.1, Vasilyeva A.D.1, Bychkova A.V.1, Pronkin P.G.1, Bugrova A.E.1, Indeykina M.I.1,2, Shilkina N.G.1, Konstantinova M.L.1, Kononikhin A.S.1,3,2, Nikolaev E.N.1,2,4, Rosenfeld M.A.1
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Affiliations:
- Emanuel Institute of Biochemical Physics
- Moscow Institute of Physics and Technology
- Institute for Energy Problems of Chemical Physics
- Skolkovo Institute of Science and Technology
- Issue: Vol 474, No 1 (2017)
- Pages: 231-235
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/211920
- DOI: https://doi.org/10.1134/S1607672917030218
- ID: 211920
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Abstract
For the first time, by using the complex of physicochemical methods (mass-spectrometry, differential scanning calorimetry, spectrofluorimetry, method of spectral and fluorescent probes, dynamic light scattering, and UV spectrophotometry), the oxidation-mediated modification of chemical and spatial structure of albumin has been studied. All albumin structural regions are subjected to oxidation, methionine and aromatic amino acids primarily involved in oxidation. The albumin melting shows a decrease in thermal stabilization of the structure and changing of aggregation upon oxidation. The change in physical and chemical properties of albumin under different quantity of the oxidizer has been analyzed.
About the authors
M. G. Gorobets
Emanuel Institute of Biochemical Physics
Author for correspondence.
Email: maria.g.gorobets@gmail.com
Russian Federation, Moscow, 119334
L. A. Wasserman
Emanuel Institute of Biochemical Physics
Email: maria.g.gorobets@gmail.com
Russian Federation, Moscow, 119334
A. D. Vasilyeva
Emanuel Institute of Biochemical Physics
Email: maria.g.gorobets@gmail.com
Russian Federation, Moscow, 119334
A. V. Bychkova
Emanuel Institute of Biochemical Physics
Email: maria.g.gorobets@gmail.com
Russian Federation, Moscow, 119334
P. G. Pronkin
Emanuel Institute of Biochemical Physics
Email: maria.g.gorobets@gmail.com
Russian Federation, Moscow, 119334
A. E. Bugrova
Emanuel Institute of Biochemical Physics
Email: maria.g.gorobets@gmail.com
Russian Federation, Moscow, 119334
M. I. Indeykina
Emanuel Institute of Biochemical Physics; Moscow Institute of Physics and Technology
Email: maria.g.gorobets@gmail.com
Russian Federation, Moscow, 119334; Dolgoprudny, Moscow oblast
N. G. Shilkina
Emanuel Institute of Biochemical Physics
Email: maria.g.gorobets@gmail.com
Russian Federation, Moscow, 119334
M. L. Konstantinova
Emanuel Institute of Biochemical Physics
Email: maria.g.gorobets@gmail.com
Russian Federation, Moscow, 119334
A. S. Kononikhin
Emanuel Institute of Biochemical Physics; Institute for Energy Problems of Chemical Physics; Moscow Institute of Physics and Technology
Email: maria.g.gorobets@gmail.com
Russian Federation, Moscow, 119334; Moscow; Dolgoprudny, Moscow oblast
E. N. Nikolaev
Emanuel Institute of Biochemical Physics; Moscow Institute of Physics and Technology; Skolkovo Institute of Science and Technology
Email: maria.g.gorobets@gmail.com
Russian Federation, Moscow, 119334; Dolgoprudny, Moscow oblast; Skolkovo, 143025
M. A. Rosenfeld
Emanuel Institute of Biochemical Physics
Email: maria.g.gorobets@gmail.com
Russian Federation, Moscow, 119334
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