Email this article Zinc finger domain of Su(Hw) protein is required for the formation of functional Su(Hw)-dependent insulator complex
- Authors: Golovnin A.K.1, Molodina V.V.1, Georgiev P.G.1, Melnikova L.S.1
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Affiliations:
- Institute of Gene Biology
- Issue: Vol 469, No 1 (2016)
- Pages: 247-252
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/211378
- DOI: https://doi.org/10.1134/S1607672916040049
- ID: 211378
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Abstract
This study is devoted to clarifying the role of Mod(mdg4)-67.2 and Su(Hw) proteins in the interaction between Su(Hw)-dependent insulator complexes and identifying the specific domains of the Su(Hw) protein required for insulation or mutual neutralization of insulators. Using genetic techniques and experiments in yeast two-hybrid system, we have demonstrated that the zinc finger domain of the Su(Hw) protein is involved in forming a functional insulator complex and cannot be replaced with the DNA-binding domain of the GAL4 protein.
About the authors
A. K. Golovnin
Institute of Gene Biology
Author for correspondence.
Email: lsm73@mail.ru
Russian Federation, ul. Vavilova 34/5, Moscow, 119334
V. V. Molodina
Institute of Gene Biology
Email: lsm73@mail.ru
Russian Federation, ul. Vavilova 34/5, Moscow, 119334
P. G. Georgiev
Institute of Gene Biology
Email: lsm73@mail.ru
Russian Federation, ul. Vavilova 34/5, Moscow, 119334
L. S. Melnikova
Institute of Gene Biology
Email: lsm73@mail.ru
Russian Federation, ul. Vavilova 34/5, Moscow, 119334
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