Examination of convalescents after West Nile fever for the specific IgM, IgG and neutralizing antibodies


如何引用文章

全文:

详细

The examination was executed in the Astrakhan region over 2013-2014 with participation of three laboratories. In 26 convalescents after West Nile Fever (WNF) with the serological confirmed diagnosis of WNF blood sera were examined. Neurological form of WNF was observed in 8 cases, and febrile form - in 8 convalescents. Sera have been collected in 243-358 days, on average, in 308 days after the beginning of a disease. For their examination there were used IFA-IgM ELISA (MAC-ELISA), IgG ELISA methods as well as neutralization test in Vero E6 cell culture. The results of the examination in 24 of 26 patients (92, 3%) for IgM antibodies to the virus WNF were negative. In two convalescents according to the laboratories in Astrakhan and Moscow in sera there were observed low titers of IgM (1:400) with minor indices of sera optical density (0.3 to 0.4) and negative results in the Volgograd Plague Control Research Institute. Serum samples of other two convalescents were weakly positive or questionable in testing in Volgograd, but were negative when examined in the Institute of Virology. Specific IgG antibodies were detected in 23 of 26 convalescents (88.5%), neutralizing in 22 of 24 (91.7 %). These data confirm the adequacy of the criteria and tactics for WNF serodiagnosis adopted in Russia based on the application of the MAC -ELISA (IFA - IgM).

作者简介

Alla Azaryan

Center of Hygiene and Epidemiology in the Astrakhan region

Email: tu_rpn@astrakhan.ru
MD, head of the Virological Laboratory 122/89, Nikolaya Ostrovskogo str., Astrakhan, 414057, Russian Federation

A. Kozlova

The N.F. Gamaleya Federal Research Centerfor Epidemiology and Microbiology

The D.I. Ivanovsky Institute of Virology 18, Gamaleya str., Moscow, 123098, Russian Federation

A. Grishanova

Center of Hygiene and Epidemiology in the Astrakhan region

122/89, Nikolaya Ostrovskogo str., Astrakhan, 414057, Russian Federation

E. Ivashchenko

Center of Hygiene and Epidemiology in the Astrakhan region

122/89, Nikolaya Ostrovskogo str., Astrakhan, 414057, Russian Federation

G. Shendo

Center of Hygiene and Epidemiology in the Astrakhan region

122/89, Nikolaya Ostrovskogo str., Astrakhan, 414057, Russian Federation

A. Kovtunuv

Management office of Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare in Astrakhan region

138, Nikolaya Ostrovskogo str., Astrakhan, 414057, Russian Federation

V. Antonov

Volgograd Plague Control Research Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Референс-центр по мониторингу за возбудителями лихорадки Западного Нила 7, Golubinskaya str., Volgograd, 400131, Russian Federation

D. Viktorov

Volgograd Plague Control Research Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Референс-центр по мониторингу за возбудителями лихорадки Западного Нила 7, Golubinskaya str., Volgograd, 400131, Russian Federation

V. Smelyansky

Volgograd Plague Control Research Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Референс-центр по мониторингу за возбудителями лихорадки Западного Нила 7, Golubinskaya str., Volgograd, 400131, Russian Federation

E. Snatenkov

Volgograd Plague Control Research Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Референс-центр по мониторингу за возбудителями лихорадки Западного Нила 7, Golubinskaya str., Volgograd, 400131, Russian Federation

I. Barkova

Volgograd Plague Control Research Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare

Референс-центр по мониторингу за возбудителями лихорадки Западного Нила 7, Golubinskaya str., Volgograd, 400131, Russian Federation

V. Larichev

The N.F. Gamaleya Federal Research Centerfor Epidemiology and Microbiology

The D.I. Ivanovsky Institute of Virology 18, Gamaleya str., Moscow, 123098, Russian Federation

A. Butenko

The N.F. Gamaleya Federal Research Centerfor Epidemiology and Microbiology

The D.I. Ivanovsky Institute of Virology 18, Gamaleya str., Moscow, 123098, Russian Federation

参考

  1. Эпидемиологический надзор за лихорадкой Западного Нила в Астраханской области, специфическая диагностика заболевания, меры общественной и личной профилактики: Методические указания. МУ 3.1.3.002-2000. Астрахань: Центр госсанэпиднадзора в Астраханской области; 2000.
  2. Мероприятия по борьбе с лихорадкой Западного Нила на территории Российской Федерации: Методические рекомендации. Волгоград: Минздрав РФ; 2002.
  3. Мероприятия по борьбе с лихорадкой Западного Нила на территории Российской Федерации: Методические рекомендации, МУ 3.1.3.2600-10. М.; 2010.
  4. Tardei G., Ruta S., Chitu V., Rossi C., Tsai T.F., Cernescu C. Evaluation of immunoglobulin M (IgM) and IgG enzyme immunoassay in serologic diagnosis of West Nile infection. J. Clin. Microbiol. 2000; 38: 2232-9.
  5. Ларичев В.Ф., Бутенко А.М., Русакова Н.В., Шишкина Л.В., Жуков А.Н., Шишкина Е.О., Львов Д.К. Показатели специфических антител у реконвалесцентов, перенесших лихорадку Западного Нила. Вопр. вирусол. 2002; 47 (6): 11-3.
  6. Roehring J.T., Nash D., Maldin B., Labowitz A. et al. Persistence of virus-reactive serum immunoglobulin M antibody in confirmed West Nile virus encephalitis cases. Emerg. Infect. Dis. 2003; 9 (3): 376-9.
  7. Tilley P.A.G., Zachary G.A., Walle R., Schnee P.F. West Nile detection and commercial assays. Emerg. Infect. Dis. 2005; 11 (7): 1154-5.
  8. Prince H.E., Tobler L.H., Yeh C., Gefter N., Custer B., Busch M.P. Persistence of West Nile virus-specific antibodies in viremic blood donors. Clin. Vaccine Immunol. 2007; 14: 1228-30.
  9. Бутенко А.М. Научная конференция Проблемной комиссии “Арбовирусы и другие вирусы зоонозов”. Актуальные вопросы изучения лихорадки Западного Нила, лихорадки денге и других завозных тропических арбовирусных инфекций в Российской Федерации. Эпидемиол. и инфекц. бол. 2014; (3): 58-61.
  10. Papa A., Anastasia A., Delianidou M. West Nile virus IgM and IgG antibodies three year post-infection. Hippokratia. 2015; 19 (1): 34-6.

版权所有 © Eco-vector, 2016


 


##common.cookie##