Antigen-presenting activity of dendritic cells in patients with chronic hepatitis C genotype


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Dendritic cells (DC) are the most professional antigen presenting cells, which are the first to recognize the «danger signals» and trigger highly specific immune responses. However, the results ofstudies performed by some foreign and domestic authors, testify to a violation of the functional activity of DCs in patients with chronic hepatitis C (CHC). In order to study the nature of the clinical course of various forms of CHC and for the assessment of the capability of DC to antigen presentation in the 2014-2015 in «Research Institute of Influenza» there was performed a simple comparative clinical study. DC obtained from patients with CHC were found to have high antigen-presenting activity due to the presence on their surface a high level of the expression of MHC class I and II. The maturation of DC is accompanied by a significant increase in the levels of expression of HLA-A, B, C and HLA-DR, irrespective of the degree of liver fibrosis, biochemical activity of alanine aminotransferase or level of viral load.

作者简介

V. Tsvetkov

Research Institute of Influenza

Email: suppcolor@gmail.com
аспирант, науч. сотр. ФГБУ «НИИ гриппа» 15/17, Professora Popova str., Saint Petersburg, Saint-Petersburg, Russian Federation, 197376

O. Kiselev

Research Institute of Influenza

Email: office@influenza.spb.ru
акад. РАН, проф., директор ФГБУ «НИИ гриппа» 15/17, Professora Popova str., Saint Petersburg, Saint-Petersburg, Russian Federation, 197376

T. Sologub

Research Institute of Influenza

Email: sologub@influenza.spb.ru
доктор мед. наук, проф. заместитель директора по научной и клинической работе ФГБУ «НИИ гриппа» 15/17, Professora Popova str., Saint Petersburg, Saint-Petersburg, Russian Federation, 197376

A. Slita

Research Institute of Influenza

Email: alexander.slita@influenza.spb.ru
канд. биол. наук, зав. лаб. клеточных культур ФГБУ «НИИ гриппа» 15/17, Professora Popova str., Saint Petersburg, Saint-Petersburg, Russian Federation, 197376

I. Tokin

Research Institute of Influenza; North-Western State Medical University named after I.I. Mechnikov

Email: ivan.i.tokin@rambler.ru
канд. мед. наук, зав. отд-нием экспериментальной терапии вирусных гепатитов ФГБУ «НИИ гриппа», доцент каф. инфекционных болезней ГБОУ ВПО «Северо-Западный государственный медицинский университет им. И.И. Мечникова» 15/17, Professora Popova str., Saint Petersburg, Saint-Petersburg, Russian Federation, 197376

E. Anhimova

Research Institute of Influenza

Email: ankhimova@influenza.spb.ru
канд. мед. наук, сотр. клиникодиагностической лаб. ФГБУ «НИИ гриппа» 15/17, Professora Popova str., Saint Petersburg, Saint-Petersburg, Russian Federation, 197376

参考

  1. Medzhitov R., Janeway C.A. Decoding the patterns of self and non-self by the innate immune system. Science. 2002; 296: 298-300.
  2. Цветков В.В., Сологуб Т.В., Токин И.И. Биология дендритных клеток человека и их роль в инфекционной патологии. Российский медико-биологический вестник имени академика И.П. Павлова. 2014; 3: 68-74.
  3. Katsuaki S., Shigeharu F. Dendritic cells-nature and classification. Allergol. Int. 2007; 56: 183-91.
  4. Liu Y.J. IPC: professional type 1 interferon-producing cells and plasmacytoid dendritic cell precursors. Annu. Rev. Immunol. 2005; 23: 275-306.
  5. Trombetta E.S., Mellman I. Cell biology of antigen processing in vitro and in vivo. Annu. Rev. Immunol. 2005; 23: 975-1028.
  6. Inaba K., Turley S., Iyoda T., Yamaide F., Shimoyama S., Reis e Sousa C. et al. The formation of immunogenic major histocompatibility complex class II-peptide ligands in lysosomal compartments of dendritic cells is regulated by inflammatory stimuli. J. Exp. Med. 2000; 191: 927-36.
  7. Tsubouchi E., Akbar S.M.F., Murakami H., Horiike N., Onji M. Isolation and functional analysis of circulating dendritic cells from hepatitis C virus (HCV) RNA-positive and HCV RNAnegative patients with chronic hepatitis C: role of antiviral therapy. Clin. Exp. Immunol. 2004; 137 (2): 417-23.
  8. Takaki A., Tatsukawa M., Iwasaki Y., Koike K., Noguchi Y., Shiraha H. et al. Hepatitis C virus NS4 protein impairs the Th1 polarization of immature dendritic cells. J. Viral Hepat. 2010; 17: 555-62.
  9. Stranska R., Florentin J., Dental C., Aouar B., Gondois-Rey F., Durantel D. et al. Hepatitis C virus fails to activate NF-kappaB signaling in plasmacytoid dendritic cells. J. Virol. 2012; 86 (2): 1090-6.
  10. Sarobe P., Lasarte J.J., Zabaleta A., Arribillaga L., Arina A., Melero I. et al. Hepatitis C virus structural proteins impair dendritic cell maturation and inhibit in vivo induction of cellular immune responses. J. Virol. 2003; 77: 10 862-71.
  11. Zimmermann M., Flechsig C., La Monica N., Tripodi M., Adler G., Dikopoulos N. Hepatitis C virus core protein impairs in vitro priming of specific T cell responses by dendritic cells and hepatocytes. J. Hepatol. 2008; 48: 51-60.
  12. Mazumdar B., Kim H., Meyer K., Bose S.K., Di Bisceglie A.M., Ray R., Hoft D.F. Hepatitis C virus-mediated inhibition of cathepsin S increases invariant-chain expression on hepatocyte surface. J. Virol. 2012; 86 (18): 9919-28.
  13. Bottomly K., Jorritsma P.J., Brogdon J.L. Role of TCR-induced extracellular signal-regulated kinase activation in the regulation of early IL-4 expression in naive CD4 T cells. J. Immunol. 2003; 170 (5): 2427-34.
  14. Tao X., Grant C., Constant S., Bottomly K. Induction of IL-4-producing CD4 T cells by antigenic peptides altered for TCR binding. J. Immunol. 1997; 158 (9): 4237-44.

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