Application of immunoassay systems for diagnosis of dengue fever


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Abstract

As a result of the application of experimental test systems "ELISA-IgM-dengue", "ELISA-IgM-WN" and "ELISA-IgM-TBE" when examining sera from patients with dengue fever (DF), West Nile fever (WNF) and tick-borne encephalitis (TBE) there was established the possibility of a clear differential diagnosis of these related flavivirus infections. Application of the ELISA-IgG test systems fail to provide such an opportunity due to cause of pronounced cross-reactions of group-IgG antibodies. In the article there are presented data on dynamics of IgM and IgG antibodies in patients with dengue fever. Detection of specific IgG in the first days of the disease may indicate to a secondary nature of infection and the possibility of the development of hemorrhagic syndrome.

About the authors

Yu. A Akinshina

ZAO "ECOlab"

Email: akinshina.opr@mail.ru
науч. сотр., микробиолог отдела перспективных разработок ЗАО «ЭКОлаб» 1, Budennogo Str., Electrogorsk, Moscow Region, Russian Federation, 142530

V. F Larichev

Research Center of Epidemiology and Microbiology Center named after Academician N.F. Gamaleya

Email: arboelisa@mail.ru
D.I. Ivanovsky Institute of Virology 16, Gamalei Str., Moscow, Russian Federation Russian, 123098

S. G Mardanly

ZAO "ECOlab"

Email: ekolab-sekretar@mail.ru
канд. мед. наук, директор по науке ЗАО «ЭКОлаб» 1, Budennogo Str., Electrogorsk, Moscow Region, Russian Federation, 142530

A. M Butenko

Research Center of Epidemiology and Microbiology Center named after Academician N.F. Gamaleya

Email: arboelisa@mail.ru
D.I. Ivanovsky Institute of Virology 16, Gamalei Str., Moscow, Russian Federation Russian, 123098

N. V Khutoretskaya

Research Center of Epidemiology and Microbiology Center named after Academician N.F. Gamaleya

D.I. Ivanovsky Institute of Virology 16, Gamalei Str., Moscow, Russian Federation Russian, 123098

M. A Saifullin

Infectious Clinical Hospital №1

Email: dr_saifullin@mail.ru
врач-инфекционист, зав. отд-нием ГБУЗ «Инфекционная клиническая больница № 1» ДЗМ 63, Volokolamskoye Sh., Moscow, Russian Federation , 123367

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