Email this article Detection of Coxiella burnetii in ticks collected from cattle in several provinces of the Republic of Guinea
- Authors: Panferova Y.A.1, Freylikhman O.A.1, Tokarevich N.K.1, Naydenova E.V.2, Zakharov K.S.2, Senichkina A.M.2, Agafonov D.A.2, Nassour A.A.3, Konstantinov O.K.3, Sanaba B.3, Boiro M.3
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Affiliations:
- Saint-Petersburg Pasteur Institute, Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance
- Russian Scientific Anti-plague Institute “Microb”
- Research Institute of Applied Biology
- Issue: Vol 24, No 5-6 (2019)
- Pages: 234-239
- Section: ORIGINAL STUDIES
- URL: https://journals.rcsi.science/1560-9529/article/view/34806
- DOI: https://doi.org/10.17816/EID34806
- ID: 34806
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Abstract
Background. Q fever, or coxiellosis, is a natural focal disease characterized by polymorphism of clinical signs and can affect not only humans but also many species of animals. This infection is spread almost all over the world. On the African continent, the foci of coxiellosis infection endanger the local population and people arriving for temporary stay. Given that sick agricultural animals and their ectoparasites are markers of the presence of infection in the region, a study of the latter may be relevant to identify the potential foci of Q fever.
This work aimed to identify Coxiella burnetii DNA from ixodic ticks collected from cattle in several provinces of Republic of Guinea and to type isolates using genetic markers (plasmid type) to enable their comparison with strains of different geographical origin.
Methods. Using amplification technologies, we investigated the ticks obtained from cattle in the provinces of Boke and Kindia to detect Coxiella DNA.
Results. The genetic material of the Q fever causative agent was detected in no more than 5% of the total number of samples studied. For positive samples, typing was performed using plasmid analysis. The isolates with the plasmid type QpH1 circulate in the Republic of Guinea.
Conclusion. The findings were analyzed along with data from other researchers on the spread of Q fever in subequatorial Africa. The differences in the levels of prevalence of Coxiella in ticks in the territories of not only different countries but also within the same state can be determined by the prevalence among the hosts within herds. The risk of contamination with Q fever in endemic regions should be considered.
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##article.viewOnOriginalSite##About the authors
Yulia A. Panferova
Saint-Petersburg Pasteur Institute, Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance
Author for correspondence.
Email: zoonoses@mail.ru
ORCID iD: 0000-0001-5211-5086
Russian Federation, Saint-Petersburg
Olga A. Freylikhman
Saint-Petersburg Pasteur Institute, Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance
Email: zoonoses@mail.ru
ORCID iD: 0000-0002-2850-728X
к.б.н., ведущий научный сотрудник
Russian Federation, Saint-PetersburgNikolay K. Tokarevich
Saint-Petersburg Pasteur Institute, Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance
Email: zoonoses@mail.ru
ORCID iD: 0000-0001-6433-3486
MD, PhD
Russian Federation, Saint-PetersburgEkaterina V. Naydenova
Russian Scientific Anti-plague Institute “Microb”
Email: zoonoses@mail.ru
ORCID iD: 0000-0001-6474-3696
PhD
Russian Federation, SaratovKirill S. Zakharov
Russian Scientific Anti-plague Institute “Microb”
Email: zoonoses@mail.ru
ORCID iD: 0000-0002-4726-309X
PhD
Russian Federation, SaratovAyslu M. Senichkina
Russian Scientific Anti-plague Institute “Microb”
Email: zoonoses@mail.ru
ORCID iD: 0000-0003-1026-2680
PhD
Russian Federation, SaratovDmitry A. Agafonov
Russian Scientific Anti-plague Institute “Microb”
Email: zoonoses@mail.ru
ORCID iD: 0000-0001-9273-6063
PhD
Russian Federation, SaratovAbdulay A. Nassour
Research Institute of Applied Biology
Email: zoonoses@mail.ru
научный сотрудник
Guinea, KindiaOleg K. Konstantinov
Research Institute of Applied Biology
Email: zoonoses@mail.ru
PhD
Guinea, KindiaBoumbaly Sanaba
Research Institute of Applied Biology
Email: zoonoses@mail.ru
PhD
Guinea, KindiaMamadou Yero Boiro
Research Institute of Applied Biology
Email: zoonoses@mail.ru
ORCID iD: 0000-0003-3339-9380
PhD
Guinea, KindiaReferences
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