In vitro studies of bioglass material by X-ray diffraction and solid-state MAS-NMR

Resumo

Bioactive glass 46S6 has been elaborated by melting method at high temperature. “In vitro” experiments of 46S6 glass were carried out by soaking in a simulated body fluid for different times. The kinetics of chemical reactivity and the bioactivity of this biomaterial was investigated by X-ray diffraction and elucidated by using an original structural analysis method based on solid-state MAS-NMR. After in vitro assays, X-ray diffraction confirmed the high bioactivity of bioglass 46S6. The ²⁹Si MAS-NMR spectra showed the emergence of two new species: Q_Si³ (OH) Q_Si⁴ and which are characteristic of the dissolution of vitreous network of 46S6 glass while the ³¹P MAS-NMR spectra highlighted the formation of new component attributed to hydroxycarbonate apatite. The bioglass 46S6 have presented the rapid formation of a biological active hydroxycarbonate apatite layer after soaking in simulated body fluid fluid.