Email this article Expression, Purification, Crystallization and X-Ray Crystallographic Analysis of MoDabb1 from Magnaporthe oryzae
- Authors: Zhao Y.1,2, Chi M.1, Zhang X.2, Wang S.2, Liu J.2, Liang W.1, Huang J.1
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Affiliations:
- College of Plant Health and Medicine, Key Lab of Integrated Crop Disease and Pest Management of Shandong Province, Qingdao Agricultural University
- College of Plant Protection, MOA Key Laboratory of Plant Pathology, China Agricultural University
- Issue: Vol 64, No 7 (2019)
- Pages: 1112-1116
- Section: Structure of Macromolecular Compounds
- URL: https://journals.rcsi.science/1063-7745/article/view/194501
- DOI: https://doi.org/10.1134/S1063774519070307
- ID: 194501
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Abstract
Protein acetylation is one of the most common post-translational modifications. Many acetylated proteins in Magnaporthe oryzae play key roles in vegetative growth and pathogenicity. MoDabb1 from M. oryzae was also identified to be an acetylated protein, containing a Dabb domain with unknown function. To elucidate the function of this protein and the effect of acetylation on this protein, a native and selenomethionine-substituted MoDabb1 were expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using sitting-drop vapour-diffusion method. Crystals of native and selenomethionine-substituted protein were diffracted to a resolution of 1.74 and 1.98 Å and both belonged to the sp. gr. P42212. Matthews coefficient analysis indicated two molecules in an asymmetric unit with a Vm value of 2.41 and a corresponding solvent content of 49.03%.
About the authors
Y. Zhao
College of Plant Health and Medicine, Key Lab of Integrated Crop Disease and Pest Management of Shandong Province, Qingdao Agricultural University; College of Plant Protection, MOA Key Laboratory of Plant Pathology, China Agricultural University
Email: jghuang@qua.edu.cn
China, Qingdao, Shandong, 266109;
Haidian District, Beijing, 100193
M. Chi
College of Plant Health and Medicine, Key Lab of Integrated Crop Disease and Pest Management of Shandong Province, Qingdao Agricultural University
Email: jghuang@qua.edu.cn
China, Qingdao, Shandong, 266109
X. Zhang
College of Plant Protection, MOA Key Laboratory of Plant Pathology, China Agricultural University
Email: jghuang@qua.edu.cn
China,
Haidian District, Beijing, 100193
S. Wang
College of Plant Protection, MOA Key Laboratory of Plant Pathology, China Agricultural University
Email: jghuang@qua.edu.cn
China,
Haidian District, Beijing, 100193
J. Liu
College of Plant Protection, MOA Key Laboratory of Plant Pathology, China Agricultural University
Email: jghuang@qua.edu.cn
China,
Haidian District, Beijing, 100193
W. Liang
College of Plant Health and Medicine, Key Lab of Integrated Crop Disease and Pest Management of Shandong Province, Qingdao Agricultural University
Email: jghuang@qua.edu.cn
China, Qingdao, Shandong, 266109
J. Huang
College of Plant Health and Medicine, Key Lab of Integrated Crop Disease and Pest Management of Shandong Province, Qingdao Agricultural University
Author for correspondence.
Email: jghuang@qua.edu.cn
China, Qingdao, Shandong, 266109
