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Polymorphism of sequences and the secondary structure of b/c intron of mitochondrial gene nad1 in Monotropa hypopitys and related ericaceae species


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Abstract

Nucleotide sequences of b/c intron of the mitochondrial gene nad1 were determined for the first time and analyzed in 24 plants of Monotropa hypopitys and in three related Ericaceae species. Two mitotypes of b/c intron of the nad1 gene, differing in sequence lengths and the presence of a number of single nucleotide substitutions and indels, were revealed in M. hypopitys. For the first time, a possible pre-mRNA secondary structure of M. hypopitys b/c intron was determined and IBS/EBS binding sites and the borders of six functional domains were identified.

About the authors

M. A. Filyushin

Bioengineering Institute, Research Center of Biotechnology

Author for correspondence.
Email: michel7753@mail.ru
Russian Federation, Leninskii pr. 33, bldg. 2, Moscow, 119071

N. M. Reshetnikova

Tsitsin Main Botanical Garden

Email: michel7753@mail.ru
Russian Federation, ul. Botanicheskaya 4, Moscow, 127276

E. Z. Kochieva

Bioengineering Institute, Research Center of Biotechnology; Department of Biotechnology

Email: michel7753@mail.ru
Russian Federation, Leninskii pr. 33, bldg. 2, Moscow, 119071; Moscow, 119991

K. G. Skryabin

Bioengineering Institute, Research Center of Biotechnology; Department of Biotechnology

Email: michel7753@mail.ru
Russian Federation, Leninskii pr. 33, bldg. 2, Moscow, 119071; Moscow, 119991

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