MOLECULAR GENETIC TESTING IN DIAGNOSTICS OF LUNG CANCER AND OVARIAN CARCINOMA WITH THE USAGE OF CYTOLOGICAL SPECIMENS


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This article presents results of the introduction in practical oncology of molecular genetic investigations performed with the use of tumor DNA cells taken from the cytological specimens. There was investigated the molecular genetic characteristics of cytological specimens from 126 patients. In 80 cases with the proved diagnosis of pulmonary adenocarcinoma (n = 80) EGFR gene mutations were noted in 11.7% cases. KRAS, BRAF and BRCA1/2 gene mutations were determined in 46 women suffering from serous ovarian carcinoma. KRAS gene mutations in cells of ovarian low-grade serous carcinoma were determined in 62.5% of patients, BRAF- in 12.5% cases. BRCA1 gene mutations have been determined in 14.3% cases from the ovarian high-grade serous carcinoma group. In conditions of the presence of the sufficient amount of tumor cells the cytological material is the fully-featured material for molecular genetic investigations. The investigation both of EGFR gene mutations in pulmonary adenocarcinoma cases and KRAS, BRAF, BRCA1/2 gene mutations with serous ovarian carcinoma are mandatory in the appointment of targeted therapy.

作者简介

Olga Grigoruk

Altai Branch of N.N. Blokhin Russian Cancer Research Center; Altai Regional Oncology Dispensary; The Altai State Medical University

Email: cytolakod@rambler.ru
MD, PhD, DSc, Head of the Clinical Laboratory Diagnostics Department (for Carrying out Cytological Research Methods) of the Altai Regional Oncological Dispensary; Barnaul, 656049, Russian Federation 656049, Barnaul, Russian Federation; Barnaul, 656049, Russian Federation; Barnaul, 656038, Russian Federation

E. Pupkova

Altai Regional Oncology Dispensary

Barnaul, 656049, Russian Federation

L. Bazulina

Altai Regional Oncology Dispensary

Barnaul, 656049, Russian Federation

A. Lazarev

Altai Branch of N.N. Blokhin Russian Cancer Research Center; Altai Regional Oncology Dispensary; The Altai State Medical University

656049, Barnaul, Russian Federation; Barnaul, 656049, Russian Federation; Barnaul, 656038, Russian Federation

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