Multiparameter study of the immunophenotype of tumor infiltrating lymphocytes, in cancer patients


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Abstract

For the first time in Russia the immunophenotype of tumor infiltrated lymphocytes (TIL) was investigated with the use of a method of quantitative flow cytometry. Samples from 104 patients, suffering from solid tumors (such as breast cancer, ovarian cancer, melanoma and oral cavity squamous carcinoma), were analyzed in this investigation. In the difference of cytomorphological analysis, the method of flow cytometry identified TIL in 100% of cases. In the structure of CDS+Т- lymphocytes the ratio CD4/CD8 was equal to 1,1±0,1. Serial gating strategy allowed to assess minor subpopulations of regulatory T-lymphocytes CD45+CD4+CD127+low/neg and CD45+CD8+CD28-CD11b-. Regulatory mechanisms with involvement of CD8 T-cells played the main role in the generation of immune responses at the tissue level ofpatients, suffering from solid tumors, independent of nosological form of disease.

About the authors

Tat’yana N. Zabotina

N.N. Blokhin Russian Cancer Research Center

Email: tatzabotina@yandex.ru
MD, Phd, DSc, leading researcher of the Laboratory of Clinical immunology of tumors of the Research Institute of Clinical Oncology Moscow, 115478, Russian Federation

O. V Korotkova

N.N. Blokhin Russian Cancer Research Center

Moscow, 115478, Russian Federation

A. A Borunova

N.N. Blokhin Russian Cancer Research Center

Moscow, 115478, Russian Federation

D. V Tabakov

N.N. Blokhin Russian Cancer Research Center

Moscow, 115478, Russian Federation

I. I Bokin

N.N. Blokhin Russian Cancer Research Center

Moscow, 115478, Russian Federation

I. V Panichenko

N.N. Blokhin Russian Cancer Research Center

Moscow, 115478, Russian Federation

M. V Savostikova

N.N. Blokhin Russian Cancer Research Center

Moscow, 115478, Russian Federation

I. V Samoylenko

N.N. Blokhin Russian Cancer Research Center

Moscow, 115478, Russian Federation

V. T Tsiklauri

N.N. Blokhin Russian Cancer Research Center

Moscow, 115478, Russian Federation

Sh. G Khakimova

N.N. Blokhin Russian Cancer Research Center

Moscow, 115478, Russian Federation

Z. G Kadagidze

N.N. Blokhin Russian Cancer Research Center

Moscow, 115478, Russian Federation

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