Laboratory criteria for improving the diagnosis of autoimmune hepatitis and Wilson’s disease in children
- 作者: Zhuzhula A.A.1, Kurbatova O.V.1, Petrichuk S.V.1, Fisenko A.P.1, Snovskaya M.A.1, Movsisyan G.B.1, Potapov A.S.1,2, Semikina E.L.1,2
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隶属关系:
- National Medical Research Center for Children’s Health
- I. Sechenov First Moscow State Medical University (Sechenov University)
- 期: 卷 28, 编号 4 (2025)
- 页面: 971-978
- 栏目: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/1028-7221/article/view/333258
- DOI: https://doi.org/10.46235/1028-7221-17275-LCF
- ID: 333258
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Specification of laboratory criteria for improving differential diagnostics of children with autoimmune hepatitis (AIH) and Wilson’s disease (WD) is a relevant task in pediatrics. Increased titers of antinuclear factor (ANF) in blood serum is among laboratory criteria for diagnostics of AIH. Some WD cases have been described which can manifest as autoimmune hepatitis (AIH) with detection of non-specific autoantibodies, thus complicating the differential diagnosis. The aim of our study was to identify the specific features for determination of antinuclear factor on the Hep-2 cell line and specific autoantibodies using a triple cell substrate in children with autoimmune hepatitis and WD. We have examined 62 pediatric patients with WD and 28 children with AIH. ANF was determined on the HEp-2 cell line (ANA-HEp-2, AESKUSLIDES®, Germany). Specific auto-antibodies were detected by means of a triple substrate obtained from rodents, which included samples of kidney, liver and stomach tissues (LKS Mouse, Germany) using indirect fluorescence reactions applying an automated analyzer HELIOS® (Germany). The analysis revealed ANF positivity in 15 out of 62 (24%) children with WD. Positive ANF results were obtained in 26 out of 28 children AIH (93%), being significantly more common in AIH compared with WD. The study of specific autoantibodies using a triple substrate revealed positive results in 34 children with WD (55%), versus 26 children with AIH (93%), Hence, the specific auto-antibodies on the triple substrate are more commonly found in children with AIH (p < 0.001) than in children with WD. In children with suspected AIH, when a homogeneous type of luminescence is detected on the HEp-2 cell line, and with a combination of different luminescence types on a triple substrate, the presence of Wilson’s disease in patients cannot be excluded.
作者简介
Anastasia Zhuzhula
National Medical Research Center for Children’s Health
编辑信件的主要联系方式.
Email: anas-zh@inbox.ru
ORCID iD: 0000-0002-6292-7229
Junior Researcher, Laboratory of Experimental Immunology and Virology
俄罗斯联邦, 2 Lomonosovsky Ave, Bldg 1, Moscow, 119991Olga Kurbatova
National Medical Research Center for Children’s Health
Email: putintseva@mail.ru
ORCID iD: 0000-0002-9213-5281
PhD (Medicine), Senior Researcher, Laboratory of Experimental Immunology and Virology
俄罗斯联邦, 2 Lomonosovsky Ave, Bldg 1, Moscow, 119991Svetlana Petrichuk
National Medical Research Center for Children’s Health
Email: cito@list.ru
ORCID iD: 0000-0003-0896-6996
PhD, MD (Biology), Professor, Chief Researcher, Laboratory of Experimental Immunology and Virology
俄罗斯联邦, 2 Lomonosovsky Ave, Bldg 1, Moscow, 119991Andrei Fisenko
National Medical Research Center for Children’s Health
Email: Putintseva@mail.ru
PhD, MD (Medicine), Professor, Director
俄罗斯联邦, 2 Lomonosovsky Ave, Bldg 1, Moscow, 119991Marina Snovskaya
National Medical Research Center for Children’s Health
Email: snows@inbox.ru
ORCID iD: 0000-0002-5263-6743
PhD (Medicine), Leading Researcher, Laboratory of Experimental Immunology and Virology
俄罗斯联邦, 2 Lomonosovsky Ave, Bldg 1, Moscow, 119991Goar Movsisyan
National Medical Research Center for Children’s Health
Email: movsisyan@nczd.ru
ORCID iD: 0000-0003-2881-4703
PhD (Medicine), Senior Researcher, Laboratory of Rare Hereditary Diseases; Gastroenterologist, Gastroenterology Department with the Hepatological Group
俄罗斯联邦, 2 Lomonosovsky Ave, Bldg 1, Moscow, 119991Alexander Potapov
National Medical Research Center for Children’s Health; I. Sechenov First Moscow State Medical University (Sechenov University)
Email: potapov@nczd.ru
ORCID iD: 0000-0003-4905-2373
PhD, MD (Medicine), Professor, Chief Researcher, Laboratory of Scientific Foundations of Pediatric Gastroenterology and Hepatology, Head of Gastroenterology Department with Hepatology Group, Professor, Department of Pediatrics and Pediatric Rheumatology
俄罗斯联邦, 2 Lomonosovsky Ave, Bldg 1, Moscow, 119991; MoscowElena Semikina
National Medical Research Center for Children’s Health; I. Sechenov First Moscow State Medical University (Sechenov University)
Email: semikinaelena@yandex.ru
ORCID iD: 0000-0001-8923-4652
PhD, MD (Medicine), Head, Laboratory Department, Professor, Department of Pediatrics and Pediatric Rheumatology
俄罗斯联邦, 2 Lomonosovsky Ave, Bldg 1, Moscow, 119991; Moscow参考
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