The involvement of proinflammatory cytokines in the pathogenesis of audiogenic epilepsy

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Epilepsy is a heterogeneous disease, which determines the relevance of investigating the mechanisms of pathogenesis of its various types, including reflex epilepsy. Pharmacotherapy is common for the treatment of patients with epilepsy, however, despite the significant recent achievements, 20-30% of patients remain resistant to the ongoing treatment. The urgency of creating new antiepileptic drugs, in particular, immune ones is due not only to a significant proportion of pharmacoresistant cases, but also to the struggle for the quality of life of patients. The neuroinflammation system in animals with different genetically determined audiogenic epilepsy proneness was investigated. These genetic groups were Krushinsky–Molodkina rats (tonic seizures of maximum intensity in response to the action of sound) and “0” strain (control group, non-convulsive phenotype). The main proinflammatory cytokines levels in the dorsal striatum and brain stem in rats of these genetic groups were measured by multiplex immunofluorescence assay. Background levels of IL-1â, IL-6 and TNFá in the dorsal striatum of KM rats were significantly lower than in the control “0” strain rats, whereas in the brain stem in the “background” levels of these metabolites did not differ. Four hours after the sound exposure, the TNFá level in the dorsal striatum of KM rats was significantly lower than in “0” rats. In KM rats, after the sound exposure and subsequent tonic seizures, the levels of IL-1â and IL-6 in the dorsal striatum were significantly higher than in the background. The IL-2 content was not detected in the background in KM rats, whereas after audiogenic seizures its level was 14.01 pg/ml. In the brain stem of KM rats, the levels of IL-1â and TNFá after audiogenic seizures were significantly lower than in the background. In rats of the “0” strain, cytokine levels in the dorsal striatum after the sound exposure did not differ from those in the background, while IL-1ß levels in their brain stem were significantly lower than the background state. Particular modulating role of the studied proinflammatory cytokines in the pathogenesis of audiogenic epilepsy is assumed, as well as certain possibility of anti-inflammatory and immune drugs application in anticonvulsant and antiepileptic therapy.

作者简介

N. Surina

Lomonosov Moscow State University

编辑信件的主要联系方式.
Email: Opera_ghost@inbox.ru

PhD (Biology), Senior Research Associate, Biology Department

俄罗斯联邦, Moscow

I. Fedotova

Lomonosov Moscow State University

Email: Opera_ghost@inbox.ru

PhD (Biology), Senior Research Associate, Biology Department

俄罗斯联邦, Moscow

I. Poletaeva

Lomonosov Moscow State University

Email: Opera_ghost@inbox.ru

PhD, MD (Biology), Leading Research Associate, Biology Department

俄罗斯联邦, Moscow

参考

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2. Figure 1. IL-1 concentrations in the brain tissues of the KM and “0” strain rats: A, dorsal striatum; B, brain stem Note. Cytokine concentrations in pg/ml are presented as Me (Q0.25-Q0.75). Background state (white columns) and 4 hours after sound exposure (gray columns). *, **, significant difference, p < 0.05 and 0.01, respectively.

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3. Figure 2. Dorsal striatum IL-2 1 I (A) and IL-6 (B) concentrations in rats of KM and “0” strain Note. As for Figure 1.

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4. Figure 3. Dorsal striatum (A) and brain stem (B) TNF concentrations in rats of KM and “0” strain Note. As for Figure 1.

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