THE STERIC AVAILABITY OF THYROID PEROXIDASE EPITOPES IN ENZYME IMMUNOASSAY FOR THE DETERMINATION OF AUTOANTIBODIES

Evaluation of circulating autoantibodies (auto-At) to thyroid peroxidase (TPO) is widely used in the clinic to identify / confirm autoimmune diseases of the thyroid gland. Despite significant progress in creating tests for auto-At detection, the issues of their validation, standardization of methods attract close attention of both developers and users. Comparative studies of the level of antibodies in the blood serum of patients with diff use toxic goiter, autoimmune thyroiditis using diagnosticumof diff erent manufacturers showed that the discrepancies are associated with the TPO epitope structure immobilized on the solid phase. It is proposed to use monoclonal antibodies to control the antigenic structure of TPO as the process of isolation of the native protein, and the production of tests for the analysis of At to TPO.