THE EFFECT OF LOCAL APPLICATION OF ERYTHROPOIETIN ON DEATH INDICATORS OF LYMPHOCYTES IN THE BLOOD IN THE DYNAMICS OF EXPERIMENTAL THERMAL INJURY

To investigate the effect of local application of erythropoietin (EPO) in the composition of transdermal film (TDF) on the death's indicators of lymphocytes in the blood in dynamics of experimental thermal trauma (TI). The experiment was made out on 90 white nonlinear rats that were divided into groups: 1 - intact rats; 2 - animals with the TI and an aseptic bandage (n=40), 3 - animals with the TI application of the TDF with EPO (n=40). Modeling TI IIIA degree and area of 3.5 % was carried out by immersing the interscapular region of the skin of the animal in clean water with a temperature of 98-99°C 12 s. In the preliminary studies was developed TDP on the basis of sodium carboxymethyl cellulose with EPO at the dose of 500 IU/g. The dressing was carried out daily. The stady was conducted for 3rd, 5th, 8th and 14th days. Death's indicators of lymphocytes was assessed by staining cells with fluorochrome conjugated with annexin V and 7-aminoantipyrine D in flow cytometer "Navios". It is established that at TI at 3, 5 and 8 day increase in the number of lymphocytes with the phenotype of the Annexin-5-FITC⁺/7-AAD^- and Annexin-5-FITC⁺/7-AAD⁺, 5 and 8 day reduced the number of lymphocytes with the phenotype of the Annexin-5-FITC^-/7-AAD^-. In the application of TDF with the EPO at the TI for 5 and 14 days reduced the number of lymphocytes with the phenotype of the Annexin-5-FITC⁺/7-AAD^-, 8 and 14 days, reduced the number of Annexin-5-FITC⁺/7-AAD⁺ cells at 5 and 14 days increased lymphocytes Annexin-5-FITC^-/7-AAD^-. Thus, in TT the application of TDP with EPO leads to a decrease in blood lymphocytes with signs of apoptosis and necrosis.

термическая травма, эритропоэтин, лимфоциты, трансдермальная пленка, thermal injury, transdermal film, erythropoietin, lymphocytes