DETECTION OF CYTOKINE-LIKE SUBSTANCES WITH THE HELP OF A TEST SYSTEM FOR THE DETERMINATION OF CYTOKINES IN A MOUSE IN STAPHYLOCOCCUS AUREUS, OPPOSITE FOR SPA GENE

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In the work, cytokine-like activity was studied in 6 strains of Staphylococcus aureus, with and without genetic determinants of staphylococcal protein A (spa gene). The spa gene in S. aureus was determined by the polymerase chain reaction (PCR) method. The production of cytokine-like substances in the supernatants of S. aureus daily broth cultures was determined using an 8-plex immunofl uorescence test system for the determination of mouse cytokines from BIO-RAD (USA) (GM-CSF, INF-γ, IL-1b, IL-2, IL-4, IL-5, IL-10, TNF-α) on the device MAGPIX-100 (USA). It was established that, regardless of the presence or absence of genetic markers of staphylococcal protein A, cytokine-like substances were detected in the supernatants of the studied strains of S. aureus. Thus, the cytokine-like activity of S. aureus, detected using a multiplex mouse test cytokine test system, does not depend on the presence of the spa gene in bacteria, which is responsible for the production of staphylococcal protein A.

作者简介

A. Zurochka

Institute of Immunology and Physiology UrB RAS;
South-Ural State University (National Research University)

编辑信件的主要联系方式.
Email: av_zurochka@mail.ru

leader scientific researcherof the laboratory of immunology of inflammation, Ekaterinburg;

MD, professor of the Department of food and biotechnology, senior researcher of the laboratory of molecular immunology, Chelyabinsk

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V. Zurochka

Institute of Immunology and Physiology UrB RAS;
South-Ural State University (National Research University)

Email: fake@neicon.ru

senior scientific researcherof the laboratory of immunology of inflammation, Ekaterinburg;

MD, professor of the Department of food and biotechnology, senior researcher of the laboratory of molecular immunology, Chelyabinsk

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L. Fomina

Institute of Immunology and Physiology UrB RAS

Email: fake@neicon.ru

post-graduate student,

Ekaterinburg

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A. Fayzullina

Institute of Immunology and Physiology UrB RAS

Email: fake@neicon.ru

post-graduate student,

Ekaterinburg

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M. Dobrynina

Institute of Immunology and Physiology UrB RAS

Email: fake@neicon.ru

Junior scientific researcherof the laboratory of immunology of inflammation,

Ekaterinburg

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V. Gritsenko

Orenburg Federal Research Сenter UrB RAS

Email: fake@neicon.ru

MD, Professor, Chief Researcher,

Orenburg

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参考

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  6. Sabat A., Krzyszton-Russjan J., Strzalka W., Filipek R., Kosowska K., Hryniewicz W., Travis J., Potempa J. Newmethod for typing Staphylococcus aureus strains: multiple-locus variable-number tandem repeat analysis of polymorphism and genetic relationships of clinical isolates. J. Clin. Microbiol. 2003. 41(4): 1801–1804.
  7. Фомина Л. О., Файзуллина А. И., Зурочка В. А., Добрынина М. А., Симбирцев А. С., Гриценко В. А. Сравнительная оценка цитокиноподобной активности Staphylococcus aureus мультиплексным и иммуноферментным анализом. Российский иммунологический журнал. 2018, 12 (21), 3, 460– 465.

版权所有 © Zurochka A.V., Zurochka V.A., Fomina L.O., Fayzullina A.I., Dobrynina M.A., Gritsenko V.A., 2019

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