EFFECTS OF PROLINE-RICH PEPTIDES ON THE FUNCTIONAL ACTIVITY OF HUMAN LEUKOCYTES IN VITRO

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The effects of peptides with a high content of proline (Proline-Rich Peptides, PRPs) of human saliva (fragments of Basic salivary proline-rich protein 1 – P-H (37–51), P-F (43–61), IB6 (98–116), p1932), as well as of several animal’s neutrophilic PRPs (bactenecins ChBac3.4, ChBac5, mini-ChBac7.5Na), towards cells involved in the functioning of the immune system have been explored. In the presence of each of the investigated PRPs the intensity of the respiratory burst of human blood phagocytes in vitro, challenged by Escherichia coli, was reduced (the effect was more pronounced in the case of salivary PRPs). Administration of ChBac3.4, mini-ChBac7.5Nα, р1932 to human peripheral blood mononuclear cells, stimulated with lipopolysaccharide, caused a decrease of LPS-mediated release of cytokines IL1β, TNFα, without affecting a level of IL10 (for other PRPs the effects were not significant). Thus, the results confirm the anti-inflammatory action of PRPs and point to the suggestion that cationic prolinerich proteins and peptides of saliva may be involved in regulation of inflammation in the oral cavity.

作者简介

A. Artamonov

FSBSI Institute of Experimental Medicine

编辑信件的主要联系方式.
Email: auartamonov@bk.ru

PhD, Research Scientist of the Department of General Pathology and Pathophysiology, 

Saint-Petersburg

俄罗斯联邦

M. Sukhareva

FSBSI Institute of Experimental Medicine;
Peter the Great Polytechnic University

Email: fake@neicon.ru

Technician of the Department of General Pathology and Pathophysiology;

Student,

Saint-Petersburg

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P. Kopeykin

FSBSI Institute of Experimental Medicine

Email: fake@neicon.ru

Junior Research Scientist of the Department of General Pathology and Pathophysiology,

Saint-Petersburg

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A. Sukhachev

FSBSI Institute of Experimental Medicine

Email: fake@neicon.ru

PhD, Research Scientist of the Department of Immunology,

Saint-Petersburg

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T. Filatenkova

FSBSI Institute of Experimental Medicine

Email: fake@neicon.ru

Research Scientist of the Department of General Pathology and Pathophysiology,

Saint-Petersburg

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D. Orlov

FSBSI Institute of Experimental Medicine

Email: fake@neicon.ru

PhD, Head of the Laboratory of Immunopathophysiology of the Department of General Pathology and Pathophysiology,

Saint-Petersburg

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O. Shamova

FSBSI Institute of Experimental Medicine

Email: fake@neicon.ru

PhD, Dr. of Sci, Head of the Department of General Pathology and Pathophysiology, 

Saint-Petersburg

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参考

  1. Вавилова Т. П., Янушев О. О., Островская И. Г. Слюна. Аналитические возможности и перспективы. М.: Издательство БИНОМ. 2014. 312 с.
  2. Merrifield R. B., Barany G. Solid-phase peptide synthesis. In: The peptides: analysis, synthesis, biology. 1980, New York: Academic Press, No 2, P. 3–284.
  3. Shi J., Ross C.R., Leto T.L., Blecha F. PR-39, a proline-rich antibacterial peptide that inhibits phagocyte NADPH oxidase activity by binding to Srchomology 3 domains of p47 phox. Proc Natl Acad Sci USA. 1996; 93(12), 6014–8.

版权所有 © Artamonov A.Y., Sukhareva M.S., Kopeykin P.M., Sukhachev A.N., Filatenkova T.A., Orlov D.A., Shamova O.V., 2019

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