Сomparative immune resistance of Enterococci and Staphylococci isolated in chronic bacterial prostatitis
- 作者: Pashinina O.A.1, Pashkova T.M.1, Kartashova O.L.1, Popova L.P.1, Kuzmin M.D.1, Gritsenko V.A.1
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隶属关系:
- Institute of Cellular and Intracellular Symbiosis – a Separate Structural Unit of the Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences
- 期: 卷 28, 编号 4 (2025)
- 页面: 1047-1054
- 栏目: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/1028-7221/article/view/333267
- DOI: https://doi.org/10.46235/1028-7221-17260-CIR
- ID: 333267
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The aim of the study was to conduct a comparative analysis of immunoresistance factors in enterococci and staphylococci strains isolated in chronic bacterial prostatitis (CBP). In vitro studies were conducted on 24 clinical strains of Gram-positive cocci of different species: Enterococcus faecalis (n = 6), Staphylococcus aureus (n = 6), coagulase-negative staphylococci (CoNS): S. haemolyticus (n = 6), S. epidermidis (n = 6), isolated from prostatic secretion in men with CBP. Antilysozyme activity (ALA) of bacteria and their ability to form biofilms (BFA) were determined photometrically; sIgA protease activity of bacteria and their anticytokine activity (ACA) in relation to pro- and anti-inflammatory cytokines (IL-6, IL-8, IL-17A, TNFα and IL-4) were determined by the enzyme immunoassay. The obtained data were processed by the methods of variation statistics and the Spearman correlation criterion. Upon the comparative analysis of immunoresistance factors in enterococci and staphylococci isolated in CBP, no significant differences in the expression levels of ALA, BPO, and ACA in relation to IL-8 and TNFα were revealed between the isolates of bacteria of the studied species, but their interspecies variability in sIgA protease expression and ACA against IL-4, IL-6, and IL-17A were noted. The results of correlation analysis have shown a close positive relationship between the expression of ACA IL-8 and ACA IL-6 in CoNS and S. aureus isolates, as well as between sIgA protease activity and ACA IL-6 in CoNS and E. faecalis strains. Inverse correlations were found between ACA to IL-8 and ACA to IL-17A, ACA IL-8 and BFA. Moreover, BFA and ACA to TNFα were detected in E. faecalis. These results allowed us to characterize a number of immunoresistance factors associated with inactivation/suppression of antimicrobial defense mechanisms in clinical strains of E. faecalis, S. aureus and CoNS of different species isolated from patients with CBP, which may contribute to the survival and long-term persistence of pathogens in the prostate gland. Further studies in this area can provide new practically useful data, including information related to the prediction of persistence for distinct bacterial strains.
作者简介
Olga Pashinina
Institute of Cellular and Intracellular Symbiosis – a Separate Structural Unit of the Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: olga25mikro@mail.ru
ORCID iD: 0000-0001-9944-3095
SPIN 代码: 9638-7336
Scopus 作者 ID: 57196410629
Researcher ID: J-3331-2018
PhD (Biology), Senior Researcher, Laboratory of Microbial Persistence and Symbiosis
俄罗斯联邦, OrenburgT. Pashkova
Institute of Cellular and Intracellular Symbiosis – a Separate Structural Unit of the Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences
Email: pashkova070782@mail.ru
ORCID iD: 0000-0001-8075-8249
SPIN 代码: 5146-6190
Scopus 作者 ID: 57189696210
Researcher ID: J-2057-2018
PhD, MD (Biology), Leading Researcher, Laboratory of Microbial Persistence and Symbiosis
俄罗斯联邦, OrenburgOlga Kartashova
Institute of Cellular and Intracellular Symbiosis – a Separate Structural Unit of the Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences
Email: labpersist@mail.ru
ORCID iD: 0000-0002-1487-7546
SPIN 代码: 9315-1070
Scopus 作者 ID: 7003322797
Researcher ID: P-2645-2015
PhD, MD (Biology), Leading Researcher, Laboratory of Microbial Persistence and Symbiosis
俄罗斯联邦, OrenburgL. Popova
Institute of Cellular and Intracellular Symbiosis – a Separate Structural Unit of the Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences
Email: labpersist@mail.ru
ORCID iD: 0000-0002-3766-6020
SPIN 代码: 8577-1007
Scopus 作者 ID: 56946322300
Researcher ID: J-3339-2018
PhD (Medicine), Senior Researcher, Laboratory of Microbial Persistence and Symbiosis
俄罗斯联邦, OrenburgM. Kuzmin
Institute of Cellular and Intracellular Symbiosis – a Separate Structural Unit of the Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences
Email: mdk-49@mail.ru
ORCID iD: 0000-0002-6349-6053
SPIN 代码: 1895-4318
PhD, MD (Medicine), Leading Researcher, Laboratory for Studying the Mechanisms of Formation of Human Microbiocenoses
俄罗斯联邦, OrenburgV. Gritsenko
Institute of Cellular and Intracellular Symbiosis – a Separate Structural Unit of the Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences
Email: vag59@mail.ru
ORCID iD: 0000-0002-2086-5170
SPIN 代码: 7107-1300
Scopus 作者 ID: 7101892654
Researcher ID: O-9429-2015
PhD, MD (Medicine), Leading Researcher, Laboratory for Studying the Mechanisms of Formation of Human Microbiocenoses
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